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DDI - Palestras

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http://hdl.handle.net/10400.18/420

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Browsing DDI - Palestras by Title

Now showing 1 - 10 of 26
  • Antibiotic resistance: where are we and where are we going?
    Publication . Manageiro, Vera
    Antibiotic resistance is an emerging problem, becoming a serious threat to global public health. Indeed, we are seeing across Europe, namely in Portugal, an increasing number of patients with multidrug-resistant (MDR), extensively-drug resistant (XDR) and pandrug-resistant (PDR) infections, meaning there is no effective treatment available. New resistance mechanisms are disseminating worldwide, and the situation is aggravated by a low investment in this area, and specifically a substantial decline in the research and development of antibacterial agents, among others. Hence, very few new antibacterial classes are brought to market when older classes lose their efficacy. Antibiotic resistance constitutes a global health priority with severe consequences to humans, animals and to the environment, and should be tackled on all fronts in view to the actual concept of “One World - One Medicine - One Health”.
    2016-11-13Lecture Restricted access Show more
  • 2016-05Lecture Restricted access Show more
  • Application of whole-genome sequencing to detect antibiotic resistance in Gram-negative bacteria
    Publication . Manageiro, Vera
    Antibiotic resistance is an emerging problem, becoming a serious threat to global public health. The causes of its spread are complex, as are the strategies to combat this threat. Following recent improvements in sequencing technologies, whole-genome sequencing (WGS) provides a comprehensive alternative in the evaluation and detection of antibiotic resistance mechanisms. In the scope of the analysis of nonsusceptibility of Gram-negative isolates recovered from human, veterinary and environment samples, we identified the presence of a high diversity of resistance mechanisms. To understand the genetic background of those resistance mechanisms, which included oxyimino-β-lactam, fluoroquinolone and colistin resistance-encoding genes, we performed whole genome and plasmid sequencing using a 454 (Roche) and/or MiSeq (Illumina) sequencing strategy. A set of bioinformatic web tools were used to estimate the presence of pathogenicity determinants, antibiotic resistance (AR) genes, and clinically relevant mobile genetic elements. Indeed, the efficient gene capture and spread of resistance determinants by mobile genetic elements are factors to be taken into account, due to their contribution for the co-selection of multidrug resistant strains in the different settings and environment. Furthermore, WGS might be used with great benefit in combination with phenotypic methods for surveillance purposes.
    2017-03-31Lecture Restricted access Show more
  • Aspergillus and aspergilosis: its relevance in the actual context
    Publication . Sabino, Raquel
    Revisão sobre o género Aspergilus, suas características morfológicas, genómicas e taxonómicas, bem como as patologias associadas a infeção por este microrganismo.
    2017-06-09Lecture Restricted access Show more
  • Aspergillus e aspergilose: sua relevância no contexto atual
    Publication . Sabino, Raquel
    Esta apresentação visa abordar aspetos clínicos das infeções por Aspergillus, diagnóstico laboratorial da aspergilose e questões emergentes relacionadas com a temática.
    2020-05-15Lecture Restricted access Show more
  • Diagnóstico laboratorial das infeções fúngicas invasivas
    Publication . Verissimo, Cristina
    As infeções fúngicas invasivas (IFI) estão associadas a elevada mortalidade. O diagnóstico numa fase inicial é determinante para o sucesso da terapia. As leveduras do género Candida são os agentes mais frequentes de infeção fúngica invasiva; No grupo dos fungos filamentosos o género Aspergillus e os fungos asseptados da ordem Mucorales são referidos como os principais agentes de IFI, no entanto outras espécies têm vindo a emergir, nomedamente Rhodotorula spp. Trichopoporon spp., Fusarium spp. Scedosporium spp. Devido à falta de métodos capazes de diagnosticar com exatidão as infeções fungicas invasivas o diagnóstico destas infeções deverá ser baseado em várias abordagens complementares que incluem: critérios clínicos, imagiologia compatível, histopatologia, cultura e métodos não culturais (pesquisa de antigénio, DNA fúngico). Os métodos moleculares têm vindo a ser melhorados de modo a assegurar reprodutibilidade intra e inter laboratorial, com vista à sua validação para diagnóstico laboratorial. Perspetivam-se novas metodologias que permitam tornar mais rápido o diagnóstico das infeções fúngicas invasivas e guidelines incluam os métodos não culturais, com vista a melhor adequação da terapia antifúngica. Nesta apresenção serão abordadas as diferentes metodologias disponiveis para o diagnóstico laboratorial das infeções fúngicas, principais vantagens e limitações.
    2019Lecture Restricted access Show more
  • Diagnóstico laboratorial em micologia
    Publication . Verissimo, Cristina
    Objetivos: 1.Principais tipos de infecção; 2.Colheita e transporte de amostras; 3.Métodos diretos (exame direto e cultural); 4.Métodos indiretos para diagnóstico de infeções fúngicas; 5.Valorização de resultados; 6.Identificação de leveduras e fungos filamentosos.
    2016Lecture Restricted access Show more
  • Etiological agents more frequently associated to Aspergillus infection: their macro and microscopic characterization
    Publication . Sabino, Raquel; Veríssimo, Cristina
    Sobre as principais caracteristicas necessárias à identificação laboratorial de Aspergillus (componente prática).
    2017-06-09Lecture Restricted access Show more
  • Fungi as agentes of Mycosis: epidemiology/ecology
    Publication . Sabino, Raquel
    2013-01-15Lecture Restricted access Show more
  • 2013-03-14Lecture Restricted access Show more