DDI - Posters/abstracts em congressos nacionais
URI permanente para esta coleção:
Navegar
Entradas recentes
- SARS-CoV-2 serological 6-months follow-up study of a hospital-based cohort of healthcare workers following 2023 COVID-19 vaccination programPublication . Santos, João Almeida; Gaio, Vânia; Amaral, Palmira; Henriques, Camila; Guiomar, Raquel; Machado, AusendaObjective: Healthcare workers (HCWs) have a higher risk of SARS-CoV-2 infection due to their direct contact with patients. On the other hand, they can also act as a source of in-hospital transmission. We assessed SARS-CoV-2 serological status of HCWs at a Portuguese central hospital before vaccination, three and six months after the 2023 COVID-19 booster vaccination program. Methods: We conducted a serological follow-up study among a cohort of HCWs from a Portuguese central hospital, with three rounds of testing: pre-COVID-19 vaccination (September/October 2023), three months (January/February 2024), and six months (April/May 2024) post-vaccination. During follow-up, only participants who underwent pre-vaccination serology and were vaccinated were included. SARS-CoV-2 spike receptor-binding domain (anti-RBD/S) protein-specific IgG antibodies were measured (upper limit of detection 40000 AU/mL). Descriptive statistics and Pearson Chi-Square test analysis were performed. Results: All participants (n=177, median age: 47 years, 77,4% females) had the complete primary COVID-19 vaccination with 78,0% having received 2 additional booster doses prior to 2023 vaccination program. At pre-vaccination, all HCWs had anti-RBD/S IgG antibodies with 11,9% (n=21) presenting a concentration >40000 AU/mL. Three months later, 35,0% HCWs (n=62) underwent retesting, with 40,3% presenting anti-RBD/S IgG antibody concentrations >40000 AU/mL. After six months, 26,6% HCWs (n=47) repeated testing, with 21,3% (n=10) having antibody concentrations >40000 AU/mL. The differences in anti-RBD/S IgG antibody concentrations between the three rounds of testing were significant. Conclusions: Three months post-COVID-19 2023 autumn vaccination, an increase in the concentration of anti-RBD/S antibodies was identified among HCW, but after six months this concentration decreased. These results are in line with the expected decay of antibodies over time after 3 months of vaccination and reinforce the importance of revaccination in HCWs.
- Serological evidence of SARS-CoV-2 infection in a hospital based cohort study of healthcare workers following the 2023 COVID-19 vaccination programPublication . Almeida Santos, João; Gaio, Vânia; Amaral, Palmira; Henriques, Camila; Guiomar, Raquel; Machado, AusendaObjective: Healthcare workers (HCWs) are a high-risk population to acquire SARS-CoV-2 infection and becoming a focus of transmission. It is therefore important to monitor these professionals, especially due to asymptomatic infections. The objective the study was to assess SARS-CoV-2 infection rates in healthcare professionals at a central Portuguese hospital using serological tests, three and six months after the 2023 COVID-19 booster vaccination program. Methods: A prospective cohort study was established through serological follow-up in a cohort of healthcare professionals from a central Portuguese hospital, with three rounds of tests: pre-COVID-19 vaccination (September/October 2023), 3 months (January/February 2024) and 6 months (April/May 2024) post-vaccination. IgG antibodies specific to the SARS-CoV-2 nucleocapsid protein (anti-N) were measured. Data was analyzed trough descriptive statistics (frequency, mean, percentages) and infection rates at each testing moment (0, 3 and 6 months). Results: At baseline, all participants (n=177, median age: 47years, 77,4% females) had the complete primary COVID-19 vaccination, with 78% having received 2 additional booster doses prior to 2023 vaccination program. Pre vaccination, 48,6% (86/177) of HCWs had detectable anti-N IgG antibodies, of which 24,4% (21/86) self-reported having had a SARS-Cov-2 infection in 2022/2023. After 3 months, 17,7% (n=11/62) had detectable anti-N IgG antibodies although being negative in the pre-vaccination testing. After 6 months, 4,3% (n=2/47) had detectable anti-N IgG antibodies but were negative in the previous two rounds. Conclusions: During the study period, several cases of SAR-CoV-2 infection (n=13) were identified serologically among the HCW monitored, without concomitant signs and symptoms that would allow the identification of a potential infection. These results support that monitoring the infection among HCW (regardless of history of symptoms) can provide valuable information for assessing the level of exposure among hospital personnel and identifying high-risk departments. This information could allow early intervention by, for example, reminding and reinforcing the importance of personal protection standards for HCWs.
- Salmonella spp. isolates in Portugal, 2024Publication . Silveira, Leonor; Caeiro, Raquel; Henriques, Ana Margarida; Tomás, Alexandra; Pista, ÂngelaBackground: Salmonella spp. remains one of the main causes of gastrointestinal infections in the European Union, only preceded by Campylobacter spp. The most common serovars of Salmonella spp. causing illness are Salmonella enterica enterica serovar Enteritidis, Typhimurium and the monophasic variant of Typhimurium (4,[5]:i:-). The aim of this study was to evaluate the Salmonella spp. isolates circulating in Portugal in 2024, accounting the number of cases, evaluating the available epidemiological data, and analysing the phenotypic and genotypic data gathered. Methods: The National Reference Laboratory received isolates from public and private hospitals and laboratories across Portugal for serotyping using the Kauffman-White-Le Minor scheme. Isolates were also subjected to antimicrobial susceptibility testing (AST) according to EUCAST recommendations, by the disc diffusion method for 18 antibiotics. Genomes of Salmonella isolates of serovars of interest were sequenced, either on a Miseq or Nextseq Illumina platform, and raw reads were submitted to Enterobase and to several CGE tools. Results: In 2024, a total of 873 isolates were received corresponding to an increase of approximately 40%, comparing to the previous year. Most cases belonged to children (n=409; 46.8%) and in residents of Lisbon and Tagus Valley (25.3%). The most common serotypes were S. monophasic Typhimurium (32.2%; n=281), S. Enteritidis (27.4%; n=281), and S. Typhiumurium (17.3%; n=151). In total, 510 isolates (58.4%) were resistant to at least one antibiotic tested. Multi-drug resistance was observed in 20.3% (177/873) of isolates, with the most common MDR serovars being S. Typhimurium (n=98; 55.4%) and its monophasic variant (n=55; 31.1%). Four isolates were ESBL producers, presenting blaDHA-1, blaCTX-M-65, blaCTX-M -9, and blaOXA-1, and one was also a carbapenemase producer, presenting both blaOXA-1 and blaOXA -48. WGS revealed several clusters, namely among the top three serovars. Conclusions: The considerable increase in cases was intrinsically connected to several clusters of the most frequent serovars, most specially of MDR isolates. To our knowledge, this was the first time a carbapenemase producer Salmonella, isolated from a human clinical sample, was detected in Portugal. Salmonella continued laboratory surveillance and antimicrobial resistance monitoring are essential for disease prevention and control.
- Antimicrobial resistance of Salmonella Typhi and Paratyphi isolates in Portugal, January 1994 - June 2025Publication . Pista, Ângela; Henriques, Ana Margarida; Caeiro, Raquel; Tomás, Alexandra; Silveira, LeonorIntrodution: Typhoid and paratyphoid fever are severe systemic infections caused by Salmonella (S.) enterica subspecies enterica serovars Typhi and Paratyphi A, B and C. Transmission occurs primarily through ingestion of food or water contamined with faeces from infected individuals or via direct person-to person contact. In Europe, enteric fever is rare and mainly associated with travel to endemic countries. The increasing prevalence of multidrug resistance (MDR), particularly in S. Typhi, poses significant therapeutic challenges. Aim: Characterization of antibiotic resistance profiles of S. Typhi and Paratyphi A, B, and C isolates received in Jan 94-Jun 25
- Contaminação de superfícies por fungos em habitação: Um risco para a saúdePublication . Júlio, Cláudia; Rosa, Nuno; Almeida, Cristina; Cano, ManuelaIntrodução: Existe suficiente evidência epidemiológica de que os ocupantes de edifícios com problemas de humidade excessiva e contaminação de superfícies por fungos, têm um risco acrescido de desenvolver sintomas respiratórios, infeções respiratórias e de exacerbar crises de asma. No interior de edifícios, a relativa abundância de diferentes espécies de fungos tende a seguir o padrão exterior, embora as concentrações interiores sejam geralmente mais baixas. Segundo o Organização Mundial de Saúde a existência de crescimento visível de bolores em superfícies é uma forte evidência da exposição humana a estes agentes (1). Objetivo: O objetivo deste estudo é evidenciar que o aparecimento de fungos em superfícies está relacionado com o aumento da concentração de esporos suspensos no ar e/ou de colónias fúngicas nas superfícies e que as espécies isoladas são, na sua maioria, semelhantes. Metodologia: Foram colhidas amostras de ar no exterior (referência) e no interior de uma habitação T2 situada no piso térreo onde, na sequência de uma rutura de canalização de águas residuais, se observou o desenvolvimento visível de bolor nas paredes, em novembro de 2022. A amostragem em duplicado de fungos viáveis no ar foi realizada utilizando o amostrador MAS-100 e placas de Agar Extrato de Malte suplementadas com cloranfenicol. Nas superfícies foram realizadas amostragens de fungos utilizando dois métodos, placas de contacto e zaragatoas posteriormente inoculadas em Agar de Extrato de Malte suplementadas com cloranfenicol. Resultados: As concentrações de fungos obtidas no ar interior da habitação, quarto e escritório, foram superiores a 10512 UFC/m3 ultrapassando as concentrações do exterior de 1356 UFC/m3. Das colónias isoladas no interior 46% têm potencial patogénico. Foram encontradas 17 estirpes diferentes (7 espécies e 10 géneros) no interior, incluindo no ar e nas superfícies e 13 (3 espécies e 10 géneros) no exterior. Conclusões: Os resultados revelaram que as concentrações de fungos obtidas no interior da habitação são no mínimo 8 vezes superiores às do Exterior, tomadas como referência. A presença de espécies como Aspergillus glaucus, Exophiala dermatitidis, Conibiobolus coronatus apenas presentes nas amostras do interior, apresentam potencial patogénico, podendo causar osteomielite e artrite séptica, infeções cutâneas ou pulmonares, doença rinofacial crónica (2, 3). Somos levados a concluir que os esporos fúngicos encontrados no ar interior têm origem nas superfícies contaminadas em virtude do excesso de água. O ar interior é um importante determinante de saúde para os ocupantes pelo que é urgente identificar, mitigar e prevenir situações de humidade persistente que potenciam o crescimento microbiano em superfícies.
- Vigilância do vírus sincicial respiratório em crianças menores de 2 anos em Portugal, 2022/23Publication . Guiomar, Raquel; Verdasca, Nuno; Melo, Aryse; Gomes, Licínia; Henriques, Camila; Dias, Daniela; Lança, Miguel; Torres, Ana Rita; Pernadas, Bernardo; Gaio, Vânia; Rodrigues, Ana PaulaO vírus sincicial respiratório (Respiratory Syncytial Virus - RSV) é um dos principais agentes etiológicos de infeções respiratórias do trato respiratório inferior, com elevado impacto na morbilidade e mortalidade em crianças menores de 5 anos. É por isso considerado um problema de saúde pública pela Organização Mundial de Saúde, com prioridade na vigilância e prevenção. Este trabalho pretende descrever e caraterizar os casos de infeção por RSV identificados no sistema de vigilância VigiRSV durante a época 2022/23.
- Remote monitoring of disease vector mosquitoes with a new optical sensor system for automatic classificationPublication . Silva, Manuel; Alves, Maria João; Amaro, Fátima; Zé-Zé, Líbia; Osório, HugoIntroduction: - Mosquitoes represent a major threat to public health given their ability to transmit several pathogens. Some species of Aedes can transmit viruses such as dengue, Zika, or chikungunya. - Prevention of vector-borne diseases largely depends on effective and sustainable vector surveillance. Objectives: - Help to develop a novel bioacustic sensor that is able to identify the mosquitoes' species in real time - Deploy the sensor in the field in Madeira and Algarve.
- REVIVE - Rede de Vigilância de Vetores: a chegada do invasor asiático, Aedes albopictusPublication . Alves, Maria João; Amaro, Fátima; Amaro F.; Osório, Hugo; Zé-Zé, Líbia; Silva, Manuel; Soares, PatriciaO programa REVIVE (Rede de Vigilância de Vectores resulta de protocolo entre a Direção-Geral da Saúde, as Administrações Regionais de Saúde do Algarve, Alentejo, Centro, Lisboa e Vale do Tejo e do Norte, o Instituto dos Assuntos Sociais e da Saúde da Madeira, a Direção Regional de Saúde dos Açores e o Instituto Nacional de Saúde Doutor Ricardo Jorge Este programa de vigilância entomológica visa - monitorizar a atividade de artrópodes hematófagos, nomeadamente mosquitos Culicidae carraças Ixodidae e flebótomos Psychodidae - caracterizar as espécies e sua ocorrência sazonal, - e identificar agentes patogénicos importantes em saúde pública.
- Association between the adrenoreceptor β2 gene and pediatric asthma severity – a study of the PACMAN cohortPublication . Caleiro, Maria Leonor; Soares, Patricia; Antunes, Marilia"Pharmacogenetics of Asthma medication in Children: Medication with Anti-Inflammatory effects" (PACMAN) is an observational retrospective pharmacy-based study carried out in 2009, in the Netherlands, aiming to investigate the effects of genetic variation on treatment response to asthma medication in children and to identify (profiles of) SNPs that characterize response phenotypes. Data on respiratory symptoms and medication use, including medication type and amount, was collected from pharmaceutical records of asthmatic children and data on the children’s sex, age, genetic traits, and ethnicity. We aimed to assess the association between asthma severity and the Arg16Glu polymorphism of the β2 adrenoreceptor gene (ADRB2). This gene is expressed in bronchial muscle cells, which is involved in the physiological response of the airway response and has been associated with clinical drug response and asthma exacerbations. We used the PACMAN data and considered the dispensing of oral corticosteroid prescriptions as a proxy of the disease severity since corticoids are commonly used in uncontrolled asthmatic states (exacerbations). We considered two different genetic models – additive and genotypic, which can be translated for analysis purposes into a numeric format, corresponding to the number of copies of the minor allele, and categorical (each genotype representing a category), respectively. We used models of the class of the Generalized Linear Mixed Models for count data with excess of zeros, namely zero-inflated and hurdle models, considering a Negative Binomial distribution to account for overdispersion. Both models included the polymorphism in the zero and count components and were adjusted for children’s baseline characteristics. In both approaches to deal with the excess of zeros, a significant effect of the polymorphism was found only in the binary component of the models. In the count component, only sex and age showed a significant effect. This points towards the existence of an effect of the polymorphism in the presence of asthma exacerbations, with not shown effect in the frequency of OCS prescription.
- Results of the Portuguese EQA program for molecular detection of SARS-CoV-2, 2020-2022Publication . Correia, Helena; Silva, Susana; Ventura, Catarina; Melo, Aryse; Guiomar, Raquel; Faria, Ana PaulaThe initial strategy for containing the spread of SARS CoV 2 was the prompt identification of COVID 19 cases. National Reference laboratory implemented the biomolecular assays to detect SARS-CoV-2. In June 2020 the Portuguese National External Quality Assessment Program (PNAEQ) in collaboration with National Reference Laboratory for Influenza and Other Respiratory Viruses (NRL) organized the first National External Quality Assessment Program (EQA) for detection of SARS CoV 2 virus by molecular methods. The majority of the rounds included extra analytical questions The aim of EQA programs is to evaluate the participant’s performance and also estimate the overall detection rate of multiple national laboratories. We aimed to evaluate the results of implementation of a national external quality assessment program for the molecular detection of SARS-CoV-2 virus during 2020-2022.