Browsing by Issue Date, starting with "2019-06-28"
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- CarotenoidesPublication . Ravasco, Francisco; Dias, Maria da GraçaCarotenoides: são substâncias químicas que incluem pigmentos amplamente difundidos na natureza. Caracterizam-se por: serem moléculas oxidáveis; exibirem cores que vão do amarelo ao vermelho; atuarem como pigmento nas plantas e nos animais; serem lipossolúveis; estar presentes nos alimentos de origem vegetal e na gema de ovo; serem precursores da síntese de retinol nos seres humanos.
- Functional networks of DIS3L2 in cancer and NMDPublication . Garcia-Moreno, Juan; Costa, Paulo J. da; Gama-Carvalho, Margarida; Matos, Paulo; Romão, LuísaIn the flow of information from DNA to mRNA to proteins, mRNAs undergo a number of processing steps, since they are synthesized in the nucleus, until they are translated in the cytoplasm. Eukaryotic cells tightly control the fidelity of this process, via quality control pathways, among them, the nonsense-mediated mRNA decay (NMD). NMD recognizes and degrades mRNAs harboring premature translation-termination codons (PTCs) and regulates normal and fully functional mRNA levels. A new branch of the NMD pathway is starting to be revealed, which is characterized by the involvement of the DIS3L2 3’ to 5’ exoribonuclease. This protein has special relevance, given its exosome-independent action and its uridylation-mediated decay. Interestingly, mutations on this ribonuclease induce deregulation of cell-cycle genes leading to a faster cell growth and decreased chromosome stability. In this project, we aim to analyze how DIS3L2 and uridylation regulate the human transcriptome, in order to shed light on how this ribonuclease is related to NMD and how its deregulation contributes to tumorigenesis. For this purpose, high-throughput mRNA sequencing has been performed in the SW480 colorectal cancer cell line depleted of DIS3L2 or DIS3L2 plus terminal uridylyl transferases (TUTases), TUT4 and TUT7. Gene ontology analysis over the set of genes up-regulated under those two conditions, show enrichment in molecular functions and biological processes related with cancer, and cell events directly implicated RNA processing and RNA degradation. Preliminary results on genetic features in the pool of deregulated transcripts also show significant differences between conditions, an important aspect that will guide us to determine grades of sensitivities in the decay of DIS3L2-subtrates. Currently, we are setting various approaches in order to unveil the role of DIS3L2 in oncogenesis and go deeper in its substrate preference.
- Estudos de Dieta Total – Resultados, Desafios, Oportunidades | Resultados do Projeto TDS piloto realizado em Portugal – parte IIPublication . Coelho, Inês; Ventura, Marta; Gueifão, Sandra; Delgado, Inês; Castanheira, IsabelVerificou-se que os resultados do estudo piloto TDS em Portugal, para os contaminantes inorgânicos prioritários são semelhantes a outros estudos TDS publicados noutros países da Europa; Os valores de Cd e Pb não excedem os valores máximos para as amostras referidas na legislação; Os valores mais altos de As foram encontrados em pescado, no entanto a espécie arsenobetaína (não tóxica) é a predominante. A metodologia analítica utilizada (ICP-MS) é adequada ao estudo TDS, em particular pelos baixos limites de deteção e quantificação. Nem sempre os alimentos com teores mais elevados nutrientes/contaminantes inorgânicos, são os mais que contribuem para a ingestão/exposição.
- Estudos de Dieta Total (TDS)Publication . Oliveira, LuísaSumário: Enquadramento; O que é um TDS?; A que objetivos pode responder?; Vantagens e desvantagens de um TDS; Porquê um TDS em Portugal?; Introdução ao projeto TDS piloto.
- Resultados do Projeto TDS piloto realizado em Portugal - Exposição a contaminantesPublication . Vasco, Elsa; Oliveira, Luísa; Dias, Maria da GraçaApresentação dos resultados do Projeto-piloto Total Diet Studies (TDS) realizado em Portugal relativo à exposição a contaminantes.
- Vitaminas lipossolúveisPublication . Ravasco, Francisco; Dias, Maria da GraçaNo âmbito do projeto europeu TDSExposure financiado pelo 7º ProgramaQuadro de Investigação e Desenvolvimento Tecnológico da Comunidade Europeia, o Instituto Nacional de Saúde Doutor Ricardo Jorge (INSA) participou num TDS piloto que visou a harmonização de metodologias a nível europeu. Foram constituídas e analisadas 227 amostras. Cada amostra foi preparada a partir de 12 subamostras, correspondendo à recolha de 2724 subamostras na área metropolitana de Lisboa (municípios a norte do rio Tejo) entre os anos de 2014 e 2015, tendo em consideração os hábitos, a sazonalidade e as quotas de mercado. As subamostras, para constituição da amostra a analisar, foram obtidas a partir da parte edível dos alimentos preparados na forma como são ingeridos.
- Elemental composition and bioaccessibility of farmed oysters (Crassostrea gigas) fed different ratios of dietary seaweed and microalgae during broodstock conditioningPublication . Cardoso, Carlos; Gomes, Romina; Rato, Ana; Joaquim, Sandra; Machado, Jorge; Gonçalves, José Fernando; Vaz‐Pires, Paulo; Magnoni, Leonardo; Matias, Domicilia; Coelho, Inês; Delgado, Inês; Castanheira, Isabel; Matos, Joana; Ozório, Rodrigo; Bandarra, Narcisa; Afonso, CláudiaThe Pacific oyster (Crassostrea gigas) culture has been expanding, thereby leading to a greater importance of hatcheries. Broodstock conditioning is very important in the hatchery process, in which diet composition may have a strong influence on the offspring production and quality. Therefore, the current study evaluated elemental composition and bioaccessibility of oysters fed different ratios of dietary seaweed (SW) and microalgae. The dietary conditioning consisted of direct replacement of microalgae by SW at four substitution levels (0%, 25%, 50%, and 100% diet). It was observed that oysters fed 100% SW had the highest levels of Be, Cu, Zn, Sr, and Cd. The most important trend was a concentration decline of most elements with progressively lower levels of SW substitution for microalgae in the feeds. No Cd or Pb hazard (contents below 1.0 mg/kg for Cd and 1.5 mg/kg for Pb) was found in oyster meat. Regarding elemental bioaccessibility, values were similar, near 100% in the cases of Cu, Br, and I. Only for Mn and Pb, bioaccessibility percentages deviated more from 100%. Indeed, the value for Pb was 50% ± 7% (initial group), and for Mn, all values were equal or lower than 29% ± 2% (final group of oysters fed microalgae). It was observed that Mn, Cd, and Pb bioaccessibility increased with a growing share of microalgal biomass in the feed. Therefore, this study showed that SW incorporation into the feed influences elemental composition and bioaccessibility of the oysters.
- Resultados Minerais TDS Na, K, Ca, P, Fe , MgPublication . Nascimento, A.; Santiago, S.; Coelho, I.; Ventura, M.; Gueifão, S.; Coelho, M.Foram analisadas 229 amostras, por ICP-OES para a determinação dos teores de minerais: Na, K, Ca, P, Fe, Mg.
- Borrelia lusitaniae infection mimicking headache, neurologic deficits, and cerebrospinal fluid lymphocytosisPublication . Vieira, J.P.; Brito, M.J.; Lopes de Carvalho, I.Headache with neurologic deficits and cerebrospinal fluid lymphocytosis (HaNDL) is a rare headache syndrome included in the Classification of Headache of the International Headache Society as a “headache attributed to non-infectious inflammatory intracranial disease.” We report one 15-year-old patient with clinical history and cerebrospinal fluid findings compatible with the diagnosis of HaNDL in whom Borrelia lusitaniae was identified in cerebrospinal fluid by polymerase chain reaction.
- Resultados do Projeto TDS piloto realizado em Portugal – Ingestão de NutrientesPublication . Dias, Maria da Graça; Vasco, Elsa; Brazão, RobertoApresentação dos resultados do Projeto-piloto Total Diet Studies (TDS) realizado em Portugal relativo à ingestão de Nutrientes.