Percorrer por autor "Lopes, Teresa"
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- Análise de dados microbiológicos de géneros alimentícios prontos a comer servidos no ano de 2013 em unidades de restauração coletivaPublication . Correia, Cristina Belo; Bonito, Conceição Costa; Barreira, Maria João; Cunha, Isabel Campos; Coelho, Anabela; Flores, Cristina; Furtado, Rosália; Lopes, Teresa; Maia, Carla; Marcos, Sílvia; Moura, Isabel; Pena, Cláudia; Santos, Susana; Sousa, Isabel; Toscano, Maria Manuel; Saraiva, Margarida; Calhau, Maria AntóniaObjetivo: Apresentar uma análise dos dados obtidos na interpretação dos ensaios microbiológicas aos géneros alimentícios prontos a comer, efetuados no âmbito dos protocolos de vigilância estabelecidos com o INSA, em 2013, de acordo com os “Valores Guia, INSA”. Não sendo critérios obrigatórios, os “Valores Guia, INSA” constituem linhas de orientação relevantes na evidência do cumprimento dos procedimentos de boas práticas em unidades de restauração coletiva.
- Caracterização genotípica e fenotípica de estirpes de Escherichia coli patogénicas, Salmonella spp. e Campylobacter spp. isoladas de aves em liberdade em Portugal continentalPublication . Batista, Rita; Saraiva, Margarida; Lopes, Teresa; Silveira, Leonor; Coelho, Anabela; Furtado, Rosália; Castro, Rita; Correia, Cristina Belo; Rodrigues, David; Henriques, Pedro; Lóio, Sara; Soeiro, Vanessa; Martins da Costa, Paulo; Oleastro, Mónica; Pista, ÂngelaAs aves são potenciais portadoras de microrganismos patogénicos que afetam os seres humanos, e podem ser disseminadoras de perigos no ambiente de produção primária de géneros alimentícios de origem vege- tal e animal. Este trabalho teve como objetivo avaliar a ocorrência, em fezes de aves em liberdade, em Portugal, de três bactérias zoonóticas causadoras de infeções no Homem. Para tal, foi avaliada a presença de Escherichia coli (E. coli), Salmonella spp. e Campylobacter spp. em 108 amostras individuais de fezes de aves e em uma amostra em pool de 50 amostras de fezes de gaivotas. Foi efetuada a caracterização fenotípica dos isolados (serotipagem e perfis de resistência a antibióticos) e dete- tados genes específicos associados à patogenicidade e à resistência a antimicrobianos, por PCR e/ou sequenciação total do genoma (WGS). Isolados de E. coli patogénicos, Salmonella spp. e Campylobacter spp. foram detetados em 8,9%, 2,8% e 9,9% das amostras, respetivamente. A resistência a antimicrobianos foi testada em 54 isolados de E. coli, tendo sido detetada em 14 (25,9%). Onze destes isolados revelaram a presença de fatores de virulência, E.coli patogénicos. Dez dos isolados de E. coli revelaram ser resistentes a múltiplos antimicrobianos (MDR) e sete eram produtores de β-lactamases de espectro alargado (ESBL). Re- lativamente aos isolados de Salmonella spp. (n=3) e Campylobacter spp. (n=9), apenas uma estirpe de Campylobacter jejuni foi identificada como MDR. A maioria dos serotipos e/ou Sequence Types (ST) identificados já tinham sido referenciados como associados a doença humana. Estes resultados mostram que as aves que fazem parte da fauna portuguesa podem ser portadoras de bactérias patogénicas capazes de causar do- ença humana, algumas delas resistentes a antimicrobianos críticos.
- Cox-2 inhibition with nutraceuticals: a new therapeutic approach against Helicobacter pylori infectionPublication . Santos, António Mário; Oleastro, Mónica; Lopes, Teresa; Pereira, Teresa; Seixas, Elsa; Chaves, Paula; Machado, Jorge; Guerreiro, António SousaAccumulated evidence in humans and animals shows that H. pylori up-regulate the expression of cyclooxygenase (COX)-2 both at mRNA and protein levels which might be one of the mechanisms leading to several gastric diseases. Aim: To study the expression of COX-2 on mice gastric mucosa during long-term treatment with two nutraceuticals: curcumin and synbiotic 2000 on H. pylori experimental chronic infection. Materials and Methods: We infected 45 C57BL/6 mice with SS1 – H. pylori strain. After infection confirmation by 13C-urea breath test mice where then treated with either PBS, curcumin (10 mg/mouse) or Synbiotic 2000 (50 mg/ mouse), three times per week. Five mice from each treatment group were euthanized at week 6, 18 and 27. Gastric samples were removed for COX-2 immunohistochemistry analysis. Results: All the 45 mice were Hp positive by 13C-urea breath test and immunohistochemistry. In the PBS group the production of COX-2 was significantly up-regulated at week 6 (area of positive immunostaining 393–544 · 103 pixels), 18 (area of positive immunostaining 242–614 · 103 pixels) and 27 week (area of positive immunostaining 129–175 · 103 pixels). The treatment with either curcumin or synbiotic significantly decreased the expression of COX-2 at all time points. Conclusions: These results suggest the therapeutic usefulness of both nutraceuticals on COX-2 inhibition during chronic experimental mice H. pylori infection. The supplementation of diet in humans with curcumin or Synbiotic 2000 may be a novel therapeutic approach against gastric inflammation induced by Hp infection.
- Escherichia coli patogénica, Salmonella spp. e Campylobacter spp. em dois Centros de Conservação da Vida Selvagem em Portugal: caracterização genotípica e fenotípicaPublication . Pista, Ângela; Silveira, Leonor; Ribeiro, Sofia; Fontes, Mariana; Castro, Rita; Coelho, Anabela; Furtado, Rosália; Lopes, Teresa; Maia, Carla; Mixão, Verónica; Borges, Vítor; Sá, Ana; Soeiro, Vanessa; Correia, Cristina Belo; Gomes, João Paulo; Saraiva, Margarida; Oleastro, Mónica; Batista, RitaA coexistência entre humanos e animais selvagens pode aumentar o risco de transmissão direta de agentes patogénicos zoonóticos emergentes ou reemergentes para humanos. Este trabalho teve como objetivo avaliar a ocorrência de três importantes agentes patogénicos de origem alimentar em animais selvagens de dois centros de conservação da vida selvagem, em Portugal. Para tal, foram testadas 132 amostras fecais para a presen- ça de Escherichia coli (E. coli produtora de toxina Shiga (STEC) e não pro- dutora de toxina Shiga (não-STEC)), Salmonella spp. e Campylobacter spp.. Foi realizada a caracterização genotípica (pesquisa de genes de vi- rulência, pesquisa de genes de resistência a antimicrobianos (AMR), se- quenciação total do genoma (WGS)) e fenotípica (serotipagem e perfis de AMR) de todos os isolados de interesse. No geral, 62 amostras testaram positivo para pelo menos uma das espé- cies analisadas: 27,3% para STEC, 11,4% para não-STEC, 3,0% para Sal- monella spp. e 6,8% para Campylobacter spp. Foi detetada resistência a antimicrobianos em quatro isolados de E. coli e no único isolado de Cam- pylobacter coli. A análise de WGS revelou que 57,7% (30/52) das E. coli patogénicas integram agrupamentos genéticos de isolados fortemente relacionados (muitas vezes envolvendo diferentes espécies de animais), indicando a existência de circulação e transmissão de diferentes estirpes patogénicas de E. coli nas áreas estudadas. Estes resultados apoiam a ideia de que a saúde dos seres humanos, dos animais e dos ecossistemas são interdependentes, reforçando a importân- cia de uma abordagem One Health (Uma Só Saúde) para melhor monitori- zar e controlar as ameaças em saúde pública
- Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland PortugalPublication . Batista, Rita; Saraiva, Margarida; Lopes, Teresa; Silveira, Leonor; Coelho, Anabela; Furtado, Rosália; Castro, Rita; Correia, Cristina Belo; Rodrigues, David; Henriques, Pedro; Lóio, Sara; Soeiro, Vanessa; da Costa, Paulo Martins; Oleastro, Mónica; Pista, AngelaBirds are potential carriers of pathogens affecting humans and agriculture. Aiming to evaluate the occurrence of the top three most important foodborne pathogens in free-living birds in Portugal, we investigated 108 individual fecal samples from free-living birds and one pooled sample of gull feces (n = 50) for the presence of Escherichia coli (pathogenic and non-pathogenic), Salmonella spp. and Campylobacter spp. Virulence- and antimicrobial resistance- (AMR) associated genes were detected by PCR and Whole-Genome Sequencing (WGS), and phenotypic (serotyping and AMR profiles) characterization was performed. Overall, 8.9% of samples tested positive for pathogenic E. coli, 2.8% for Salmonella spp., and 9.9% for Campylobacter spp. AMR was performed on all pathogenic isolates and in a fraction of non-pathogenic E. coli, being detected in 25.9% of them. Ten of the tested E. coli isolates were multidrug-resistant (MDR), and seven of them were Extended-spectrum β-lactamase (ESBL) producers. Among Salmonella (n = 3) and Campylobacter (n = 9), only one strain of C. jejuni was identified as MDR. Most of the identified serotypes/sequence types had already been found to be associated with human disease. These results show that free-living birds in Portugal may act as carriers of foodborne pathogens linked to human disease, some of them resistant to critically important antimicrobials.
- Helicobacter pylori infection: the role of intestinalPublication . Machado, Jorge; Gato, Inês; Lopes, Teresa; Pereira, Teresa; Santos, Mário; Oleastro, Mónica; Chaves, Paula; Guerreiro, AntónioIntestinal microbiota may influence inflammation in the host. The aim of the present study was to explore the role of modulation of intestinal microbiota in the outcome in the Helicobacter pylori (Hp) gastric inflammation. Twenty five C57BL/6 male mice were separated in three groups: Control group (CG) n = 5 Infected group (IG) n = 10 and Synbiotic 2000TM (SG) n = 10. CG received PBS by gavage; IG and SG were inoculated intragastrically with H. pylori SS1 cell suspension (109 CFU/mL). Then, mice were treated either with PBS (CG and IG) or Synbiotic 2000TM (SG). Five mice from each group were sacrificed at week 6 and the other at week 18. At each time samples were collected from: gastric tissue to immunohistochemistry and histological evaluation (HE) and faeces to evaluate intestinal microbiota composition by FISH, targeting 14 bacterial groups. IG and SG groups were H. pylori positive by immunohistochemistry. Microbiota analysis: In IG there were significant changes in the microbiota composition, comparing to CG. At week 6 there were changes in 12 of 14 (85.7%) bacterial groups, while at week 18 there was a change in 6/14 (42.9%). In SG, there were changes in 7/14 (50.0%) at week 6, and in 4/14 (28.6%) at week 18, comparing to CG. Histology: IG at weeks 6 and 18 has 40% (2/5) of intramucosal inflammation and SG at the same end points has 0% (0/5). These results suggest that modulation of the intestinal microbiota by Synbiotic 2000TM may influence the outcome of Hp gastric inflammation.
- Investigação laboratorial de toxinfeções alimentares, 2013Publication . Viegas, Silvia; Cunha, Isabel; Correia, Cristina; Coelho, Anabela; Maia, Carla; Pena, Cláudia; Bonito, Conceição; Sousa, Isabel; Toscano, M. Manuel; Furtado, Rosália; Santos, Susana; Lopes, Teresa; Saraiva, Margarida
- Nutraceuticals: a new therapeutic approach againstPublication . Santos, António Mário; Oleastro, Mónica; Vale de Gato, Inês; Lopes, Teresa; Seixas, Elsa; Machado, Jorge; Guerreiro, António SousaBackground and Aim: H. pylori induces severe gastric chronic inflammation and is the cause of gastritis, peptic ulcer and a major risk factor for gastric cancer. The aim of the study was to investigate the anti-inflammatory effect of two nutraceuticals in Hp-infected mucosa. Materials and Methods: Eighteen C57BL/6 mice were inoculated with Hp SS1 by gavage three times with 3 · 109 viable cells. Mice were then treated with either PBS, curcumin (10 mg/mouse) or Symbiotic 2000 (50 mg/mouse), three times per week. Half of the infected and three non-infected mice were euthanized at week 6, the remaining at week 18. Gastric samples were removed for immunohistochemistry and PCR array (inflammatory response and immunity pathway) analysis (Sabiosciences, Qiagen). Results: All the 18 mice were Hp positive by immunohistochemistry. The production of the chemokines CCL2, CCL5, CCL20, CCL25, CXCL1 and CXCL11 was significantly up-regulated at both week 6 (range of fold-change 4.3–718) and week 18 (range of fold-change 16–1192). Similarly, the expression of the proinflammatory cytokines IL-1b, IL6, IL9, IL10, IL23, TNFa and INFc was significantly augmented (range of fold-change 1338–8251). The treatment with either curcumin or symbiotic drastically decreased the expression of all these mediators, restoring their levels to those similar to the non-infected mice. Conclusions: The present study confirmed that Hp infection induces a strong inflammatory response. Curcumin and Symbiotic treatments exerted a significant anti-inflammatory effect in Hp-infected mucosa. The supplementation of diet with these nutraceuticals may be a novel clinical approach against gastric inflammation induced by Hp infection.
- Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic CharacterizationPublication . Pista, Angela; Silveira, Leonor; Ribeiro, Sofia; Fontes, Mariana; Castro, Rita; Coelho, Anabela; Furtado, Rosália; Lopes, Teresa; Maia, Carla; Mixão, Verónica; Borges, Vítor; Sá, Ana; Soeiro, Vanessa; Correia, Cristina Belo; Gomes, João Paulo; Saraiva, Margarida; Oleastro, Mónica; Batista, RitaHuman–wildlife coexistence may increase the potential risk of direct transmission of emergent or re-emergent zoonotic pathogens to humans. Intending to assess the occurrence of three important foodborne pathogens in wild animals of two wildlife conservation centers in Portugal, we investigated 132 fecal samples for the presence of Escherichia coli (Shiga toxin-producing E. coli (STEC) and non-STEC), Salmonella spp. and Campylobacter spp. A genotypic search for genes having virulence and antimicrobial resistance (AMR) was performed by means of PCR and Whole-Genome Sequencing (WGS) and phenotypic (serotyping and AMR profiles) characterization. Overall, 62 samples tested positive for at least one of these species: 27.3% for STEC, 11.4% for non-STEC, 3.0% for Salmonella spp. and 6.8% for Campylobacter spp. AMR was detected in four E. coli isolates and the only Campylobacter coli isolated in this study. WGS analysis revealed that 57.7% (30/52) of pathogenic E. coli integrated genetic clusters of highly closely related isolates (often involving different animal species), supporting the circulation and transmission of different pathogenic E. coli strains in the studied areas. These results support the idea that the health of humans, animals and ecosystems are interconnected, reinforcing the importance of a One Health approach to better monitor and control public health threats.
- Preliminary characterization of Vibrio cholerae strains isolated from seafood samples marketed in PortugalPublication . Lopes, Teresa; Lopes, David; Moura, Isabel Bastos; Sousa, Isabel; Rodrigues, João; Fernandes, Camila; Barreira, Maria João; Maia, Carla; Correia, Cristina Belo; Pintado, Cristina; Saraiva, Margarida; Batista, Rita; ElsevierVibrio cholerae, a natural inhabitant of aquatic ecosystems, has been related with gastrointestinal infections, particularly those associated with seafood consumption. This preliminary study aimed to evaluate the presence and characteristics of Vibrio cholerae, in seafood marketed in Portugal, given its potential role as a foodborne pathogen. Thus, the occurrence of Vibrio cholerae in 129 seafood raw samples (105 of shrimp and 24 of oysters), marketed in Portugal, was assessed. Isolates’ characterization regarding the presence of antimicrobial resistance (AMR) genes and of pathogenicity-specific genetic traits was attained by whole-genome sequencing (WGS). Core-genome Multi Locus Sequence Typing (cgMLST) analyses to evaluate the genetic relatedness among the isolates, and with other V. cholerae strains isolated in the world, as well as phenotypic AMR (performed by disc diffusion), were also attained. Overall, 43/129 (33.3 %) of the samples tested positive for V. cholerae (41/105 (39.1 %) of the shrimp and 2/24 (8.3 %) of the oysters’ samples). WGS analyses classified the studied strains as non-O1/non-O139 Vibrio cholerae (NOVC), lacking the main cholerae virulence factors encoded by the CTX phage. However, they carry diverse virulence factors similar to those found in O1 and O139 strains and/or in NOVC clinical strains. Furthermore, eight strains were classified as multidrug-resistant (MDR). The cg-MLST analyses revealed six genetic clusters among the 43 isolates (three identified sequence types - ST829, ST833, ST1085). Although it was not possible to find a close genetic relatedness between the studied V. cholerae strains and other deposited in PuBMLST database, a high genetic proximity among some strains isolated in different countries and from different sources (environmental and human) was observed, reinforcing the importance of a One Health approach. Assessing occurrence, pathogenic potential and genetic relatedness of Vibrio cholerae strains in the Portuguese food supply chain, this study contributes to understand their public health significance and supports a One Health approach to prevent foodborne outbreaks, contributing to food safety.