Cox-2 inhibition with nutraceuticals: a new therapeutic approach against Helicobacter pylori infection

Santos, António Mário; Oleastro, Mónica; Lopes, Teresa; Pereira, Teresa; Seixas, Elsa; Chaves, Paula; Machado, Jorge; Guerreiro, António Sousa

http://hdl.handle.net/10400.18/1327

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Authors

Santos, António Mário

Guerreiro, António Sousa

Abstract(s)

Accumulated evidence in humans and animals shows that H. pylori up-regulate the expression of cyclooxygenase (COX)-2 both at mRNA and protein levels which might be one of the mechanisms leading to several gastric diseases. Aim: To study the expression of COX-2 on mice gastric mucosa during long-term treatment with two nutraceuticals: curcumin and synbiotic 2000 on H. pylori experimental chronic infection. Materials and Methods: We infected 45 C57BL/6 mice with SS1 – H. pylori strain. After infection confirmation by 13C-urea breath test mice where then treated with either PBS, curcumin (10 mg/mouse) or Synbiotic 2000 (50 mg/ mouse), three times per week. Five mice from each treatment group were euthanized at week 6, 18 and 27. Gastric samples were removed for COX-2 immunohistochemistry analysis. Results: All the 45 mice were Hp positive by 13C-urea breath test and immunohistochemistry. In the PBS group the production of COX-2 was significantly up-regulated at week 6 (area of positive immunostaining 393–544 · 103 pixels), 18 (area of positive immunostaining 242–614 · 103 pixels) and 27 week (area of positive immunostaining 129–175 · 103 pixels). The treatment with either curcumin or synbiotic significantly decreased the expression of COX-2 at all time points. Conclusions: These results suggest the therapeutic usefulness of both nutraceuticals on COX-2 inhibition during chronic experimental mice H. pylori infection. The supplementation of diet in humans with curcumin or Synbiotic 2000 may be a novel therapeutic approach against gastric inflammation induced by Hp infection.