Publication
Cox-2 inhibition with nutraceuticals: a new therapeutic approach against Helicobacter pylori infection
| dc.contributor.author | Santos, António Mário | |
| dc.contributor.author | Oleastro, Mónica | |
| dc.contributor.author | Lopes, Teresa | |
| dc.contributor.author | Pereira, Teresa | |
| dc.contributor.author | Seixas, Elsa | |
| dc.contributor.author | Chaves, Paula | |
| dc.contributor.author | Machado, Jorge | |
| dc.contributor.author | Guerreiro, António Sousa | |
| dc.date.accessioned | 2013-02-12T19:55:42Z | |
| dc.date.available | 2013-02-12T19:55:42Z | |
| dc.date.issued | 2012-09 | |
| dc.description | Abstract no.: P3.7 publicado em: Helicobacter 2012;17(Suppl.1):98 | por |
| dc.description.abstract | Accumulated evidence in humans and animals shows that H. pylori up-regulate the expression of cyclooxygenase (COX)-2 both at mRNA and protein levels which might be one of the mechanisms leading to several gastric diseases. Aim: To study the expression of COX-2 on mice gastric mucosa during long-term treatment with two nutraceuticals: curcumin and synbiotic 2000 on H. pylori experimental chronic infection. Materials and Methods: We infected 45 C57BL/6 mice with SS1 – H. pylori strain. After infection confirmation by 13C-urea breath test mice where then treated with either PBS, curcumin (10 mg/mouse) or Synbiotic 2000 (50 mg/ mouse), three times per week. Five mice from each treatment group were euthanized at week 6, 18 and 27. Gastric samples were removed for COX-2 immunohistochemistry analysis. Results: All the 45 mice were Hp positive by 13C-urea breath test and immunohistochemistry. In the PBS group the production of COX-2 was significantly up-regulated at week 6 (area of positive immunostaining 393–544 · 103 pixels), 18 (area of positive immunostaining 242–614 · 103 pixels) and 27 week (area of positive immunostaining 129–175 · 103 pixels). The treatment with either curcumin or synbiotic significantly decreased the expression of COX-2 at all time points. Conclusions: These results suggest the therapeutic usefulness of both nutraceuticals on COX-2 inhibition during chronic experimental mice H. pylori infection. The supplementation of diet in humans with curcumin or Synbiotic 2000 may be a novel therapeutic approach against gastric inflammation induced by Hp infection. | por |
| dc.identifier.uri | http://hdl.handle.net/10400.18/1327 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.relation | FCT PTDC ⁄ SAU-OSM ⁄ 66323 ⁄ 2006 | por |
| dc.subject | Helicobacter pylori Infection | por |
| dc.subject | COX)-2 | por |
| dc.subject | Nutraceuticals | por |
| dc.title | Cox-2 inhibition with nutraceuticals: a new therapeutic approach against Helicobacter pylori infection | por |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Ljubljana, Eslovênia | por |
| oaire.citation.title | 25th International Workshop on Helicobacter and Related Bacteria in Chronic Digestive Inflammation and Gastric Cancer, September 13-15 2012 | por |
| rcaap.rights | openAccess | por |
| rcaap.type | conferenceObject | por |