Browsing by Author "Botelho, M."
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- CarcinogenesisPublication . Botelho, M.; Teixeira, João PauloCancer, or neoplasia, is a complex disease with multiple causes. Many intrinsic and extrinsic factors influence the development of cancer. Intrinsic or host factors include age, sex, genetic constitution, immune system function, metabolism, hormone levels, and nutritional status. Extrinsic factors include substances eaten, drunk, or smoked; workplace and environmental (air, water, and soil) exposures; natural and medical radiation exposure; sexual behavior; and elements of lifestyle such as social and cultural environment, personal behavior, and habits. Intrinsic and extrinsic factors can interact with one another to influence the development of cancer. In this article, we will discuss all the varied aspects of research that will ultimately lead to the prevention of cancer in man.
- Carcinogenic liver fluke Opisthorchis viverrini oxysterols detected by LC-MS/MS survey of soluble fraction parasite extractPublication . Vale, N.; Gouveia, M.J.; Botelho, M.; Sripa, B.; Suttiprapa, S.; Rinaldi, G.; Gomes, P.; Brindley, P.J.; Correia da Costa, J.M.Liquid chromatography in tandem mass spectrometry (LC-MS/MS) has emerged as an informative tool to investigate oxysterols (oxidized derivatives of cholesterol) in helminth parasite associated cancers. Here, we used LC-MS/MS to investigate in soluble extracts of the adult developmental stage of Opisthorchis viverrini from experimentally infected hamsters. Using comparisons with known bile acids and the metabolites of estrogens, the LC-MS data indicated the existence of novel oxysterol derivatives in O. viverrini. Most of these derivatives were ramified at C-17, in similar fashion to bile acids and their conjugated salts. Several were compatible with the presence of an estrogen core, and/or hydroxylation of the steroid aromatic ring A, hydroxylation of both C-2 and C-3 of the steroid ring and further oxidation into an estradiol-2,3-quinone.
- Effects of Trihalomethanes on Liver MitochondriaPublication . Faustino-Rocha, A.I.; Rodrigues, D.; Gil da Costa, R.M.; Dinis, C.; Talhada, D.; Aragão, S.; Botelho, M.; Colaço, A; Pires, M.J.; Oliveira, M.M.; Peixoto, F; Oliveira, P.A.Introduction: Trihalomethanes (THMs), namely dibromochloromethane (DBCM) and bromodichloromethane (BDCM), are disinfection byproducts of chlorinated water. This experiment aimed to evaluate the mitochondrial dysfunction induced by THMs at low levels in a mouse model.
- Mouse model of Schistosomiasis: infection with Schistosoma mansoni in CD-1 micePublication . Luis, C.; Soares, R.; Fernandes, R.; Botelho, M.Schistosomiasis is a parasitic disease that affects almost 240 million worldwide. CD1 mice were infected with cercariae of S. mansoni, after which infection developed for 8 weeks. Tissues were processed to immuno-histological techniques. It was performed H&E staining for overall analyses, Sirius Red for fibrosis and immunohistochemistry for inflammation biomarkers. The most infected organ was the liver, fibrosis decreased with egg development and Galectin-3 (Gal3) and Interleukin 6 (IL-6) were expressed inside granulomas
- Open access journals: transparent science or shady business?Publication . Botelho, M.; Richter, J.OA journals consequences for Science/ The scientific community; OA journals advantages/disadvantages for the publisher/reader/author; What can be done?
- Schistosoma mansoni infection impairs reproduction in micePublication . Botelho, M.; Lopes, G.; Gartner, F.; Alves, H.; Richter, J.AIM: To study the effect of S. mansoni infection in fertility using a mouse model. BACKGROUND: - Our group is listed in the Top 10 most publishing authors in the field of schistosomiasis as a cause of infertility; - Estrogen-like molecules produced by schistosomes haematobia and mansoni induce hormonal imbalances in infected persons; - These estrogenic metabolites down-regulate Estrogen Receptor in an in vitro model; - We have identified these estrogenic metabolites as catechol-estrogens (oxidative metabolites derived from estrogens); - These catechol-estrogens were found to be associated with infertility in women infected with S. haematobium.
- Trihalomethanes in liver pathology: Mitochondrial dysfunction and oxidative stress in the mousePublication . Faustino-Rocha, A.I.; Rodrigues, D.; da Costa, R.G.; Diniz, C.; Aragão, S.; Talhada, D.; Botelho, M.; Colaço, A.; Pires, M.J.; Peixoto, F.; Oliveira, P.A.Trihalomethanes (THMs) are disinfection byproducts found in chlorinated water, and are associated with several different kinds of cancer in human populations and experimental animal models. Metabolism of THMs proceeds through enzymes such as GSTT1 and CYP2E1 and gives rise to reactive intermediates, which form the basis for their toxic activities. The aim of this study was to assess the mitochondrial dysfunction caused by THMs at low levels, and the resulting hepatic histological and biochemical changes in the mouse. Male ICR mice were administered with two THMs: dibromochloromethane (DBCM) and bromodichloromethane (BDCM); once daily, by gavage, to a total of four administrations. Animals were sacrificed four weeks after DBCM and BDCM administrations. Blood biochemistry was performed for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TB), albumin (Alb), total protein (TP), creatinine, and urea. Animals exposed to DBCM and BDCM showed elevated ALT and TB levels (p < 0.05) as compared with controls. Histological analysis confirmed the presence of vacuolar degenerescence and a multifocal necrotizing hepatitis in 33% of animals (n = 2). Mitochondrial analysis showed that THMs reduced mitochondrial bioenergetic activity (succinate dehydrogenase (SQR), cytochrome c oxidase (COX), and ATP synthase) and increased oxidative stress (glutathione S-transferase (GST)) in hepatic tissues (p < 0.05). These results add detail to the current understanding of the mechanisms underlying THM-induced toxicity, supporting the role of mitochondrial dysfunction and oxidative stress in liver toxicity caused by DBCM and BDCM. © 2015 Wiley Periodicals, Inc. Environ Toxicol, 2015.