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Publicação
Carcinogenic liver fluke Opisthorchis viverrini oxysterols detected by LC-MS/MS survey of soluble fraction parasite extract
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Orientador(es)
Resumo(s)
Liquid chromatography in tandem mass spectrometry (LC-MS/MS) has emerged as an informative tool to investigate oxysterols (oxidized derivatives of cholesterol) in helminth parasite associated cancers. Here, we used LC-MS/MS to investigate in soluble extracts of the adult developmental stage of Opisthorchis viverrini from experimentally infected hamsters. Using comparisons with known bile acids and the metabolites of estrogens, the LC-MS data indicated the existence of novel oxysterol derivatives in O. viverrini. Most of these derivatives were ramified at C-17, in similar fashion to bile acids and their conjugated salts. Several were compatible with the presence of an estrogen core, and/or hydroxylation of the steroid aromatic ring A, hydroxylation of both C-2 and C-3 of the steroid ring and further oxidation into an estradiol-2,3-quinone.
Descrição
Palavras-chave
Bile Acids Flukes Opisthorchis Viverrini Oxysterols
Contexto Educativo
Citação
Parasitol Int. 2013 Dec;62(6):535-42. doi: 10.1016/j.parint.2013.08.001. Epub 2013 Aug 20.
Editora
Elsevier/ Japanese Society of Parasitology