Browsing by Author "Torres, Vukosava Milic"
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- Occupational secondhand smoke exposure may modify the proteoma expression of human nasal epitheliumPublication . Neves, Sofia; Pacheco, Solange; Vaz, Fátima; Torres, Vukosava Milic; James, Peter; Simões, Tânia; Penque, DeborahThe tobacco is one of the biggest public health threats, smoking kills more than 7 million people/year worldwide and more than 890,000 are deaths resulting from exposure to Second Hand Smoke (SHS). In adults, SHS is associated to cardiovascular and respiratory diseases, including coronary heart disease and lung cancer, through pathological and molecular mechanisms not yet understood. We aimed to investigate the SHS effects on airway proteome in exposed workers. Nasal epithelium was collected from hospitality workers (non-smokers=40; smokers=12), long-term exposed and non-exposed to SHS at the workplace. Samples were analyzed by shotgun proteomics using an ESI-LQT Orbitrap XL mass spectrometer. The generated MS raw data was submitted to ‘PatternLab for Proteomics’ for peptide identification and relative quantification by label-free - extracted ion chromatograms (XIC). Golden rules were applied to obtain reliable data: in the identification of a protein at least one unique peptide must had to be present in more than 80% of the individuals, and consequently each inferred protein had to be detected in 80% to 100% of the cohort. Two proteins were found to be differentially expressed in the no-smokers exposed to SHS compared with the control: BPI fold-containing family A member 1 (BPIFA1) and Heat shock Protein Beta-1 (HSPB1). The first protein plays a role in the airway inflammatory response after exposure to irritants substances and the second is associated as a regulator of actin filament dynamics. Our findings support the indication that in non-smokers the prolonged exposure to SHS can lead to airway proteome modulation. When validated, the uncovered proteins can be promising candidates to “susceptibility/risk” and/or “predictive” biomarkers for SHS exposure.
- Occupational smoke exposure may modify the expression of respiratory tract proteomaPublication . Neves, Sofia; Pacheco, Solange; Vaz, Fatima; Torres, Vukosava Milic; James, Peter; Simões, Tânia; Penque, DeborahQuestion: Tobacco is one of the biggest public health threats. Smoking kills more than 7 million people/year worldwide and more than 890,000 of deaths resulted from exposure to Second Hand Smoke (SHS). SHS is associated to cardiovascular and respiratory diseases, including coronary heart disease and lung cancer, through pathological and molecular mechanisms not yet understood. In Portugal, the partial tobacco smoking ban legislation in public venues allows smoking in restaurants with designed rooms larger than 100m2. We aimed to investigate the SHS effects on the respiratory tract proteome from exposed workers. Methods: Nasal epithelia was collected from hospitality workers (non-smokers=40; smokers=12), long-term exposed and non-exposed to SHS at the workplace. All subjects were healthy with normal spirometry values (FVE1/FVC <70%) for pulmonary diseases. Non-smokers exposed presented higher levels of urinary cotinine after working, confirming SHS exposure. Samples were analyzed by shotgun proteomics using ESI-LQT Orbitrap XL MS and the generated MS raw data by the PatternLab for Proteomics. Proteins were investigated by DAVIDv6.8 and Reactome Pathway databases. Results: Two proteins were found to be differentially expressed in the nonsmokers exposed to SHS compared with control: Heat shock Protein Beta-1 (HSPB1) and BPI fold-containing family A member 1 (BPIFA1). HSPB1 plays a role in the stress resistance and actin organization and BPIFA1 is involved in the airway inflammatory response after exposure to irritants by attracting macrophages and neutrophils, and it is also associated with tumor progression. Conclusions: In health non-smokers, prolonged exposure to SHS can lead to respiratory tract proteome modulation associated with airway inflammatory/stress response to toxic substances. When validated, the uncovered proteins can be promising candidate biomarkers of exposure/effect for clinical assessment of workers occupationally exposed to SHS.
- Proteomics in the assessment of the therapeutic response of antineoplastic drugs: strategies and practical applicationsPublication . Torres, Vukosava Milic; Popovic, Lazar; Vaz, Fatima; Penque, DeborahUncovering mechanisms of unknown pathological mechanisms and body response to applied medication are the drive forces toward personalized medicine. In this post-genomic era, all eyes are tuned to proteomic field, searching for the answers and explanations by investigating the final physiological functional units – proteins and their proteoforms. Development of cutting-edge mass spectrometric technologies and powerful bioinformatics tools, allowed life-science community mining of disease-specific proteins as biomarkers, which are often hidden by high complexity of the samples and/or small abundance. Nowadays, there are several proteomics-based approaches to study the proteome. This chapter focuses on gold standard proteomics strategies and related issues towards candidate biomarker discovery, which may have diagnostic/prognostic as well as mechanistic utility.
- Proteomics of Red Blood Cells from Patients with Obstructive Sleep ApneaPublication . Feliciano, Amelia; Bozanic, Vesna; Torres, Vukosava Milic; Matthiesen, Rune; Carvalho, Ana S.; Almeida, Andreia; Alexandre, Bruno; Vaz, Fátima; Malhotra, Atul; Pinto, Paula; Bárbara, Cristina; Penque, DeborahObstructive sleep apnea (OSA) is a common public health concern causing metabolic and cardiovascular consequences. Although OSA is a systemic disease, the molecular mechanisms and specific genes/proteins associated with such processes remain poorly defined.To identify dysregulated proteins that could be useful as candidate biomarkers of diagnosis/prognosis of OSA., is the aim of this study