Browsing by Author "Bordalo, A."
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- Could Estradiol be used as a biomarker of infection in Schistosoma haematobium infected patients?Publication . Botelho, M.C; Cardoso, R.; Bordalo, A.; Alves, H.; Richter, J.Urogenital schistosomiasis is a chronic infection caused by the human blood fluke Schistosoma haematobium. Schistosomiasis haematobia is a known risk factor for cancer leading to squamous cell carcinoma of the urinary bladder (SCC). This is a neglected tropical disease endemic in many countries of Africa and the Middle East. Schistosome eggs produce catechol-estrogens. These estrogenic molecules are metabolized to active quinones that cause alterations in DNA (leading in other contexts to breast or thyroid cancer). Our group has shown that schistosome egg associated catechol estrogens induce tumor-like phenotypes in urothelial cells, originated from parasite estrogen-host cell chromosomal DNA adducts and mutations. Also we have demonstrated that these molecules are detected as Estradiol in sera of infected patients.
- CYP2D6 and IL-6 C-174G variants in schistosomiasis haematobiaPublication . Cardoso, R.; Alves, H.; Bordalo, A.; Richter, J.; Botelho, M.C.Aim: Study polymorphic variants in CYP2D6 and the C-174G promoter polymorphism of the IL-6 gene on S. haematobium infected patients from and endemic area of Guinea Bissau. Background: - Schistosome egg associated catechol estrogens induce tumor-like phenotypes in urothelial cells and might cause schistosomiasis associated infertility (Botelho et al, Trends in Parasitol, 2015); - The cytochrome P450 (CYP) genes are involved in estrogen biosynthesis and metabolism and generation of DNA damaging procarcinogens (Blackburn et al, Cancer Causes and Control, 2015); - Very high or very low levels of IL-6 are associated with estrogen metabolism imbalance.
- CYP2D6 and IL-6 c-174G variants in schistosomiasis haematobiaPublication . Cordeiro, R.; Alves, H.; Bordalo, A.; Richter, J; Botelho, M.C.AIM: To study the polymorphic variants in CYP2D6 and the C-174G promoter polymorphism of the IL-6 gene on S. haematobium infected patients from an endemic area of Guinea Bissau. BACKGROUND: - Our group has shown that schistosome egg associated catechol estrogens induce tumor-like phenotypes in urothelial cells. These estrogen metabolites might also be the cause of schistosomiasis associated infertility; - The cytochrome P450 (CYP) genes are oxygenases involved in estrogen biosynthesis and metabolism, generation of DNA damaging procarcinogens, and response to anti-estrogen therapies; - IL6 Interleukin-6 (IL-6) is a pleiotropic cytokine expressed in many tissues. These cytokine is largely expressed in female urogenital tract as well as reproduction organs. Very high or very low levels of IL-6 are associated with estrogen metabolism imbalance.
- Estrogen Metabolism-Associated CYP2D6 and IL6-174G/C Polymorphisms in Schistosoma haematobium InfectionPublication . Cardoso, R.; Lacerda, P.C.; Costa, P.P.; Machado, A.; Carvalho, A.; Bordalo, A.; Fernandes, R.; Soares, R.; Richter, J.; Alves, H.; Botelho, M.C.Schistosoma haematobium is a human blood fluke causing a chronic infection called urogenital schistosomiasis. Squamous cell carcinoma of the urinary bladder (SCC) constitutes chronic sequelae of this infection, and S. haematobium infection is accounted as a risk factor for this type of cancer. This infection is considered a neglected tropical disease and is endemic in numerous countries in Africa and the Middle East. Schistosome eggs produce catechol-estrogens. These estrogenic molecules are metabolized to active quinones that induce modifications in DNA. The cytochrome P450 (CYP) enzymes are a superfamily of mono-oxygenases involved in estrogen biosynthesis and metabolism, the generation of DNA damaging procarcinogens, and the response to anti-estrogen therapies. IL6 Interleukin-6 (IL-6) is a pleiotropic cytokine expressed in various tissues. This cytokine is largely expressed in the female urogenital tract as well as reproductive organs. Very high or very low levels of IL-6 are associated with estrogen metabolism imbalance. In the present study, we investigated the polymorphic variants in the CYP2D6 gene and the C-174G promoter polymorphism of the IL-6 gene on S. haematobium-infected children patients from Guine Bissau. CYP2D6 inactivated alleles (28.5%) and IL6G-174C (13.3%) variants were frequent in S. haematobium-infected patients when compared to previously studied healthy populations (4.5% and 0.05%, respectively). Here we discuss our recent findings on these polymorphisms and whether they can be predictive markers of schistosome infection and/or represent potential biomarkers for urogenital schistosomiasis associated bladder cancer and infertility.