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Cellular, Molecular and Genotoxic Effects of Digested Titanium Dioxide Nanomaterials

2022-08-31Conference object Restricted access
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dc.contributor.authorRolo, Dora
dc.contributor.authorPereira, Joana F.S
dc.contributor.authorVieira, Adriana
dc.contributor.authorRoque, Rossana
dc.contributor.authorGramacho, Ana Catarina
dc.contributor.authorVital, Nádia
dc.contributor.authorMatos, Paulo
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorBettencourt, Ana F.
dc.contributor.authorSilva, Mafalda A.
dc.contributor.authorMartins, Carla
dc.contributor.authorAssunção, Ricardo
dc.contributor.authorAlvito, Paula
dc.contributor.authorJordan, Peter
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2022-11-28T13:01:22Z
dc.date.available2022-11-28T13:01:22Z
dc.date.issued2022-08-31
dc.descriptionAbstract publicado em: Environ Mol Mutagen. 2022 Aug;63(Suppl 1):88. (Abstracts from the 13th International Conference on Environmental Mutagens and 53rd Annual Meeting of the Environmental Mutagenesis and Genomics Society). https://onlinelibrary.wiley.com/toc/10982280/2022/63/S1pt_PT
dc.description.abstractHuman exposure to titanium dioxide nanomaterials(TiO2NMs) occurs particularly by ingestion, due to food/food contact materials and consumer products. However, the possibility of adverse effects in gastrointestinal tract is unclear. Aiming to study the impact of digestion on the NMs’ properties and their cellular/molecular effects, two human intestinal cell lines were used, Caco-2 and HT29-MTX-E12. After exposure to TiO2NMs(NM-102, NM-103, NM-105), undigested or subjected to standardized static in vitro digestion method (mimicking human digestion), the cells were analyzed for toxicity, genotoxicity, reactive oxygen species, NM uptake and intestinal translocation. We showed that in vitro digestion of TiO2NMs may increase their toxicity and DNA-damaging effect, depending on the NM, more relevant for the rutile/anatase NM-105, possibly due to its smaller hydrodynamic size in the cellular medium. Effects on chromosomal integrity were seen in HT29-MTX-E12 cells, for all tested TiO2NMs, especially after digestion. Internalization into early endosomes was confirmed for NM-103 and NM-105, before and after digestion, in monolayers of both cell lines, and at the apical membrane of polarized Caco-2 cells. The internalized NMs accumulated in late endosomes/multivesicular bodies, partially transversing the basolateral membrane of polarized Caco-2 cells without changing transepithelial electrical resistance or epithelial marker abundance. These results suggest that part of the TiO2NMs can be transcytosed through colonic epithelia without disrupting intestinal barrier integrity. Overall, the biological outcomes from TiO2NMs interaction with intestinal cells were more pronounced after digestion, highlighting its relevance in the hazard assessment of ingested NMs.pt_PT
dc.description.sponsorshipFunded by FCT/MCTES through the projects: PTDC/SAU-PUB/29481/2017, UIDB/00009/2020; UIDP/00009/2020;UIDP/50017/2020+UIDB/50017/2020+LA/P/ 0094/2020; CEECIND/03143/2017 (LG), 2020.07168.BD (NV).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8340
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationUIDB/00009/2020;pt_PT
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relationNot Available
dc.relationToxicological profile of cellulose nanomaterials in human gastrointestinal cells
dc.subjectIngested Nanomaterialspt_PT
dc.subjectNanotoxicologypt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectVias de sinalização e patologias associadas’pt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleCellular, Molecular and Genotoxic Effects of Digested Titanium Dioxide Nanomaterialspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleNot Available
oaire.awardTitleToxicological profile of cellulose nanomaterials in human gastrointestinal cells
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03143%2F2017%2FCP1476%2FCT0005/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.07168.BD/PT
oaire.citation.conferencePlaceOttawa, Canadapt_PT
oaire.citation.title13th International Conference on Environmental Mutagens (ICEM) / 53rd Annual Meeting of the Environmental Mutagenesis and Genomics Society (EMGS), 28 aug - 1 sep 2022pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2017
person.familyNameSilva
person.familyNameLouro
person.givenNameMaria Joao
person.givenNameHenriqueta
person.identifier157627
person.identifier.ciencia-id7710-643D-97A3
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0002-6060-0716
person.identifier.orcid0000-0001-9744-7332
person.identifier.scopus-author-id55944437600
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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