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Publication
Caraterização molecular e suscetibilidade aos antimicrobianos de isolados clínicos de Listeria monocytogenes na região de Lisboa e Vale do Tejo em 2015
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Advisor(s)
Abstract(s)
Listeria monocytogenes é o agente causal da listeriose, uma doença
grave considerada de vigilância prioritária a nível europeu, com taxas de
hospitalização (98,9%) e de letalidade (15,0%) elevadas. Este estudo teve
como objetivo a caraterização das estirpes de L. monocytogenes recebidas
no Instituto Nacional de Saúde Doutor Ricardo Jorge em 2015. A serotipagem
foi realizada por PCR multiplex, a tipagem por PFGE e também
foi estudada a suscetibilidade aos antimicrobianos. O serotipo mais
comum foi IVb (85,7%). A maioria das estirpes é sensível aos antimicrobianos
em apreciação, com exceção de uma estirpe que apresentou resistência
ao meropenemo e outra que apresentou resistência à eritromicina
e ao trimetoprim/sulfametoxazol. Existe uma grande heterogeneidade de
perfis de PFGE entre os isolados estudados, tendo sido detetados alguns
clusters. A utilização de sequenciação de genoma completo para caraterização
de estirpes, nomeadamente de L. monocytogenes está a ganhar
terreno a nível mundial, vindo substituir as técnicas goldstandard. A sua
aplicação no Laboratório Nacional de Referência de Infeções Gastrintestinais
permitiu em 2015 a confirmação laboratorial de um surto.
Listeria monocytogenes is the listeriosis agent, a serious disease under priority surveillance in Europe, with high mortality (15.0%) and hospitalization rates (98.9%). This study aims to characterize the Listeria monocytogenes strains received in the National Reference Laboratory of Gastrointestinal Infections of the National Institute of Health Dr. Ricardo Jorge in 2015. Serotyping was accomplished by multiplex PCR, typing by PFGE and the antimicrobial susceptibility was evaluated. The most common serotype was IVb (85.7%). Most strains are sensitive to the antimicrobials under consideration, with the exception of a strain that was resistant to meropenem and other that was resistant to erythromycin and trimethoprim/sulfamethoxazole. There is great heterogeneity between the PFGE profiles of the isolates under study, and some clusters have been detected. The use of whole sequencing for the characterization of strains namely L. monocytogenes surveillance is gaining ground worldwide, replacing the goldstandard techniques. Its application in the National Reference Laboratory of Gastrointestinal Infections allowed the effective resolution of an outbreak in 2015.
Listeria monocytogenes is the listeriosis agent, a serious disease under priority surveillance in Europe, with high mortality (15.0%) and hospitalization rates (98.9%). This study aims to characterize the Listeria monocytogenes strains received in the National Reference Laboratory of Gastrointestinal Infections of the National Institute of Health Dr. Ricardo Jorge in 2015. Serotyping was accomplished by multiplex PCR, typing by PFGE and the antimicrobial susceptibility was evaluated. The most common serotype was IVb (85.7%). Most strains are sensitive to the antimicrobials under consideration, with the exception of a strain that was resistant to meropenem and other that was resistant to erythromycin and trimethoprim/sulfamethoxazole. There is great heterogeneity between the PFGE profiles of the isolates under study, and some clusters have been detected. The use of whole sequencing for the characterization of strains namely L. monocytogenes surveillance is gaining ground worldwide, replacing the goldstandard techniques. Its application in the National Reference Laboratory of Gastrointestinal Infections allowed the effective resolution of an outbreak in 2015.
Description
Keywords
Listeriose Listeria monocytogenes Caraterização Molecular Suscetibilidade aos Antimicrobianos Vigilância Laboratorial Infecções Gastrointestinais Resistência aos Antimicrobianos Saúde Pública Lisboa e Vale do Tejo Portugal
Pedagogical Context
Citation
Boletim Epidemiológico Observações. 2016 setembro-dezembro;5(17):19-22
Publisher
Instituto Nacional de Saúde Doutor Ricardo Jorge, IP