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Occupational secondhand smoke exposure may modify the proteoma expression of human nasal epithelium

2019-05-08Lecture Restricted access
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dc.contributor.authorNeves, Sofia
dc.contributor.authorDias, Deodália
dc.contributor.authorPenque, Deborah
dc.date.accessioned2020-06-03T08:32:30Z
dc.date.available2020-06-03T08:32:30Z
dc.date.issued2019-05-08
dc.description.abstractThe tobacco is one of the biggest public health threats, smoking kills more than 7 million people/year worldwide and more than 890,000 are deaths resulting from exposure to Second Hand Smoke (SHS). In adults, SHS is associated to cardiovascular and respiratory diseases, including coronary heart disease and lung cancer, through pathological and molecular mechanisms not yet understood. We aimed to investigate the SHS effects on airway proteome in exposed workers. Nasal epithelium was collected from hospitality workers (non-smokers=40; smokers=12), long-term exposed and non-exposed to SHS at the workplace. Samples were analyzed by shotgun proteomics using an ESI-LQT Orbitrap XL mass spectrometer. The generated MS raw data was submitted to ‘PatternLab for Proteomics’ for peptide identification and relative quantification by label-free - extracted ion chromatograms (XIC). Golden rules were applied to obtain reliable data: in the identification of a protein at least one unique peptide must had to be present in more than 80% of the individuals, and consequently each inferred protein had to be detected in 80% to 100% of the cohort. Two proteins were found to be differentially expressed in the no-smokers exposed to SHS compared with the control: BPI fold-containing family A member 1 (BPIFA1) and Heat shock Protein Beta-1 (HSPB1). The first protein plays a role in the airway inflammatory response after exposure to irritants substances [1] and the second is associated as a regulator of actin filament dynamics [2]. Our findings support the indication that in non-smokers the prolonged exposure to SHS can lead to airway proteome modulation. When validated, the uncovered proteins can be promising candidates to “susceptibility/risk” and/or “predictive” biomarkers for SHS exposure. Aluno: Sofia Maria Sentieiro Neves Orientador: Professora Doutora Deodália Dias, Faculdade de Ciências da Universidade de Lisboa Doutora Deborah Penque, Instituto Nacional de Saúde Dr.º Ricardo Jorge - Lisboapt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/6895
dc.language.isoengpt_PT
dc.subjectTobaccopt_PT
dc.subjectOccupational Smoke Exposurept_PT
dc.subjectGenómica Funcionalpt_PT
dc.subjectGenómica Funcional e Estruturalpt_PT
dc.titleOccupational secondhand smoke exposure may modify the proteoma expression of human nasal epitheliumpt_PT
dc.typelecture
dspace.entity.typePublication
oaire.citation.conferencePlaceLisboa, Portugalpt_PT
oaire.citation.titleSeminário Doutoral I (2º ano), no âmbito do Programa Doutoral de Biologia (Biologia Molecular), Faculdade de Ciências da Universidade de Lisboa, 8 maio 2019pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typelecturept_PT

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