Browsing by Issue Date, starting with "2019-05-08"
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- Proteome profiling in obstructive sleep apnea severity and treatment response towards early diagnosis and prognosis predictionPublication . Coelho-Valentim, Cristina; Deodália, Dias; Penque, DeborahObstructive Sleep Apnea (OSA) syndrome is a common public health concern characterized by recurrent episodes of apneas and hypopneas during sleep. These obstructive events result in recurrent intermittent hypoxia and sleep fragmentation that can lead to metabolic and cardiovascular diseases. We recently demonstrated that OSA syndrome can cause alterations in the red blood cells (RBC) proteome that may be associated with OSA outcomes. Here we intend to investigate whether the positive airway pressure (PAP) treatment can revert/modulate these proteome alterations. RBCs from Snorers and patients with severe OSA before/after 6 months of PAP treatment (n=10/condition) were depleted of hemoglobin, analyzed by 2D-DIGE using Progenesis SameSpots v4.5. The differentially abundant proteins were identified by MALDI-MS and protein annotations acquired by DAVIDv6.8. Western blotting (WB) validation was performed for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and (overoxidized) GAPDHSO3 on a new Cohort (n=59). Statistical analysis including correlation studies with peroxiredoxin 2 (PRDX2) redox-oligomeric forms and several clinical parameters was carried out using SPSS software. Ten protein spots exhibited significant differences (Anova p<0.05) among groups and were associated with cell death, protein oligomerization and response to stress. Three proteoforms of GAPDH were identified decreased in OSA RBC (Anova p<0.05). Six months of PAP treatment increased these GAPDH proteoforms to the control levels. By WB, we confirmed these data by showing that the decreased GAPDH monomeric/tetrameric forms in OSA were increased by PAP treatment. PAP also increased GAPDHSO3 tetramers. In OSA, GAPDH monomers and GAPDHSO3 tetramers correlated positively with the respiratory disturbance index or triglycerides and adrenalin, respectively. After PAP, GAPDHSO3 tetramers correlated positively with PAP-induced PRDX2SO2/3 decameric forms, described having chaperone activity in cell protection. OSA induces alterations in the redox/oligomeric state of GAPDH and PRDX2 that can be reverted/modulated by PAP treatment. The clinical significant of these findings needs further validation and investigation. Selected Reaction Monitoring (SRM) and bioinformatics – based tools, will be used to validate the obtained data. The same proteomics workflow strategy will be applied to investigate the plasma proteome in OSA and OSA response to therapy
- Preservatives and Sweeteners: safety, benefits and occurrence in foodstuffs and table-top sweetenersPublication . Vasco, Elsa; Costa, João; Serra, CelesteNo abstract
- Laboratório Microbiologia DAN Actividades essenciais nas áreas da Microbiologia e Segurança alimentarPublication . Correia, Cristina BeloSumário: 1. Apresentação do INSA, I.P. e das actividades do Laboratório de Microbiologia do Departamento de Alimentação e Nutrição. 2. Protocolos de vigilância microbiológica em unidades de alimentação colectiva. 3. Acreditação de ensaios. 4. Amostras de alimentos prontos para consumo e superfícies do ambiente de produção: colheita, ensaios microbiológicos e interpretação de resultados. 5. Critérios microbiológicos: 5.1. Tipos de critérios 5.2. Ensaios a realizar (indicadores de alteração e de higiene e patogénicos) 5.3. Planos de amostragem 5.4. Limites a aplicar. 5.5. Métodos de análise. 5.6. Formas de expressão de resultados. 5.7. Onde aplicar os critérios. 5.8. Medidas a tomar. 6. Ensaios analíticos para verificação da conformidade com o Regulamento (EU) N.º 2073/2005. 7. Sistema RASFF. 8. Investigação de surtos (epidemiológica, laboratorial, ambiental). 8.1. Inquérito INSA para estudo laboratorial de toxinfecções alimentares. 8.2. Comunicação dos dados que chegam ao conhecimento do INSA à European Food Safety Authority (EFSA) e dados reportados em 2017.
- Occupational secondhand smoke exposure may modify the proteoma expression of human nasal epitheliumPublication . Neves, Sofia; Dias, Deodália; Penque, DeborahThe tobacco is one of the biggest public health threats, smoking kills more than 7 million people/year worldwide and more than 890,000 are deaths resulting from exposure to Second Hand Smoke (SHS). In adults, SHS is associated to cardiovascular and respiratory diseases, including coronary heart disease and lung cancer, through pathological and molecular mechanisms not yet understood. We aimed to investigate the SHS effects on airway proteome in exposed workers. Nasal epithelium was collected from hospitality workers (non-smokers=40; smokers=12), long-term exposed and non-exposed to SHS at the workplace. Samples were analyzed by shotgun proteomics using an ESI-LQT Orbitrap XL mass spectrometer. The generated MS raw data was submitted to ‘PatternLab for Proteomics’ for peptide identification and relative quantification by label-free - extracted ion chromatograms (XIC). Golden rules were applied to obtain reliable data: in the identification of a protein at least one unique peptide must had to be present in more than 80% of the individuals, and consequently each inferred protein had to be detected in 80% to 100% of the cohort. Two proteins were found to be differentially expressed in the no-smokers exposed to SHS compared with the control: BPI fold-containing family A member 1 (BPIFA1) and Heat shock Protein Beta-1 (HSPB1). The first protein plays a role in the airway inflammatory response after exposure to irritants substances [1] and the second is associated as a regulator of actin filament dynamics [2]. Our findings support the indication that in non-smokers the prolonged exposure to SHS can lead to airway proteome modulation. When validated, the uncovered proteins can be promising candidates to “susceptibility/risk” and/or “predictive” biomarkers for SHS exposure. Aluno: Sofia Maria Sentieiro Neves Orientador: Professora Doutora Deodália Dias, Faculdade de Ciências da Universidade de Lisboa Doutora Deborah Penque, Instituto Nacional de Saúde Dr.º Ricardo Jorge - Lisboa