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Abstract(s)
Human Papillomavirus (HPV) is the etiological agent for cervical cancer and genital warts. Worldwide, cervical cancer is the fourth most common cancer in women and the high risk HPV (HR-HPV), namely HPV 16 and 18 are responsible for the most of the cases. The objective was to analyze the HR-HPV frequency in a group of women referred for HR-HPV testing.
Clinical samples from 3117 women were perform by Cobas® HPV test (Roche Molecular Systems, CA, USA), this assay detected HPV 16 and HPV 18 and ‘Other HR-HPV’ (-31,-33,-35,-39,-45,-51,-52,-56,-58,-59,-66 and 68). Positive samples for ‘Other HR-HPV’ were sequenced for genotyping using MY09/11 primer´s.
HR-HPV frequency was 20.8% (649/3117). Among the positive samples, ‘Other HR-HPV’ was the most common (72.8%; 473/649). HPV 16 and 18 were detected only in 22.8% (148/649) and 7.4% (48/649) of the cases, respectively. 7.4% (48/649) of the positive women were infected with more than one HPV (34 with ‘Other HR-HPV’ + HPV 16; 8 with ‘Other HR-HPV’ + HPV 18; 5 with ‘Other HR-HPV’ + HPV 16 + HPV 18 and 1 with HPV 16 + HPV 18). Sequencing of ‘Other HR-HPV’ is ongoing and preliminary results shown the majority frequency for HPV 31 (11.7%) followed by HPV 56 (9.1%) and 8.9% for the HPV 66.
The HR-HPV frequency is high (20.8%), 30.4 % of these women were infected with HPV 16 or HPV 18 which is a high frequency. This study reveals the importance of the implementation of screening programs, and the use of HPV detection.
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Keywords
Human Papillomavirus High Risk Human Papillomavirus Portuguese Women Infecções Sexualmente Transmissíveis