Loading...
Publication
Caracterização genómica de Salmonella Newport: identificação de clusters genéticos, janeiro 1991-maio 2024
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 5.2 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Salmonella enterica subsp. enterica serovar Newport (S. Newport) é um serotipo
pouco frequente na União Europeia. No entanto, devido à sua associação
com alguns surtos de origem alimentar, a sua importância não deve
ser negligenciada. Em Portugal, os dados sobre a infeção por S. Newport
são escassos.
Este estudo teve como principais objetivos caracterizar fenotípica e genotipicamente
isolados clínicos de S. Newport entre 1991 e maio de 2024, e identificar
possíveis clusters genéticos.
Após serotipagem, 98 isolados foram submetidos a testes de suscetibilidade
aos antibióticos e sequenciação total do genoma.
Apesar da maioria dos isolados estudados (96,9%) terem sido suscetíveis
aos antibióticos testados, em 2024 foi identificado um isolado multirresistente,
que, entre outros, continha o gene blaDHA-1. A análise core genome
multilocus sequence typing (Enterobase cgMLST V2 + HierCC V1), considerando
um threshold de cinco diferenças alélicas, permitiu identificar oito
clusters distintos, dois dos quais de maiores dimensões: o Cluster 1, com
32 isolados de 2019, e o Cluster 2, com quatro isolados de 2022 e 18 de
2023.
Embora S. Newport seja um serotipo pouco frequente em Portugal e não
apresentar, na maioria dos casos, resistências aos antibióticos, é essencial
manter uma vigilância laboratorial contínua, que permita de forma atempada
implementar medidas de prevenção e controlo eficazes.
Salmonella enterica subsp. enterica serovar Newport (S. Newport) is a relatively uncommon serotype within the European Union. Nevertheless, due to the relevance of some related outbreaks, its significance should not be underestimated. In Portugal, data on S. Newport infection is scarce. The objectives of this study were to characterise clinical isolates of S. Newpor t received between Januar y 1991 and May 2024, and to identif y potential genetic clusters. Following serotyping, 98 isolates were subjected to antibiotic susceptibility testing and whole genome sequencing. Most of the isolates (96.9%) were susceptible to the tested antibiotics. However, a multidrug-resistant isolate was identified in 2024, which contained the blaDHA-1 gene, among others. The core genome multilocus sequence typing analysis (Enterobase cgMLST V2 + HierCC V1), with a threshold of five allelic dif ferences, enabled the identification of eight clusters, two of them considered as major clusters: Cluster 1, comprising 32 isolates from 2019, and Cluster 2 including four isolates from 2022 and 18 from 2023. Although S. Newpor t is an uncommon serotype in Por tugal and most of the isolates do not exhibit antibiotic resistance, it is essential to maintain a continuous laborator y surveillance programme in order to implement ef fective prevention and control measures.
Salmonella enterica subsp. enterica serovar Newport (S. Newport) is a relatively uncommon serotype within the European Union. Nevertheless, due to the relevance of some related outbreaks, its significance should not be underestimated. In Portugal, data on S. Newport infection is scarce. The objectives of this study were to characterise clinical isolates of S. Newpor t received between Januar y 1991 and May 2024, and to identif y potential genetic clusters. Following serotyping, 98 isolates were subjected to antibiotic susceptibility testing and whole genome sequencing. Most of the isolates (96.9%) were susceptible to the tested antibiotics. However, a multidrug-resistant isolate was identified in 2024, which contained the blaDHA-1 gene, among others. The core genome multilocus sequence typing analysis (Enterobase cgMLST V2 + HierCC V1), with a threshold of five allelic dif ferences, enabled the identification of eight clusters, two of them considered as major clusters: Cluster 1, comprising 32 isolates from 2019, and Cluster 2 including four isolates from 2022 and 18 from 2023. Although S. Newpor t is an uncommon serotype in Por tugal and most of the isolates do not exhibit antibiotic resistance, it is essential to maintain a continuous laborator y surveillance programme in order to implement ef fective prevention and control measures.
Description
Keywords
Salmonella Newport Caracterização Genómica Clusters genéticos Doenças Infecciosas Portugal
Pedagogical Context
Citation
Boletim Epidemiológico Observações. 2023 mai-ago;12(34):10-15
Publisher
Instituto Nacional de Saúde Doutor Ricardo Jorge, IP