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- In vitro toxicity assessment of paints with antimicrobial properties - a comparative studyPublication . Machado-Querido, M.; Teixeira, J.P.; Pereira, C. CostaIn this work, the in vitro cytotoxicity of self-disinfecting wall paints containing antimicrobial substances was assessed, using skin and lung cell lines.
- Socioeconomic differences in food habits among 6- to 9-year-old children from 23 countries-WHO European Childhood Obesity Surveillance Initiative (COSI 2015/2017)Publication . Fismen, Anne‐Siri; Buoncristiano, Marta; Williams, Julianne; Helleve, Arnfinn; Abdrakhmanova, Shynar; Bakacs, Márta; Bergh, Ingunn Holden; Boymatova, Khadichamo; Duleva, Vesselka; Fijałkowska, Anna; García‐Solano, Marta; Gualtieri, Andrea; Gutiérrez‐González, Enrique; Hejgaard, Tatjana; Huidumac‐Petrescu, Constanta; Hyska, Jolanda; Kelleher, Cecily C.; Kierkegaard, Lene; Kujundžić, Enisa; Kunešová, Marie; Milanović, Sanja Musić; Nardone, Paola; Nurk, Eha; Ostojic, Sergej M.; Ozcebe, Lütfiye Hilal; Peterkova, Valentina; Petrauskiene, Ausra; Pudule, Iveta; Rakhmatulleoeva, Sanavbar; Rakovac, Ivo; Rito, Ana Isabel; Rutter, Harry; Sacchini, Elena; Stojisavljević, Dragana; Farrugia Sant'Angelo, Victoria; Shengelia, Lela; Spinelli, Angela; Spiroski, Igor; Tanrygulyyeva, Maya; Usupova, Zhamilya; Weghuber, Daniel; Breda, JoãoBackground: Socioeconomic differences in children's food habits are a key public health concern. In order to inform policy makers, cross-country surveillance studies of dietary patterns across socioeconomic groups are required. The purpose of this study was to examine associations between socioeconomic status (SES) and children's food habits. Methods: The study was based on nationally representative data from children aged 6-9 years (n = 129,164) in 23 countries in the World Health Organization (WHO) European Region. Multivariate multilevel analyses were used to explore associations between children's food habits (consumption of fruit, vegetables, and sugar-containing soft drinks) and parental education, perceived family wealth and parental employment status. Results: Overall, the present study suggests that unhealthy food habits are associated with lower SES, particularly as assessed by parental education and family perceived wealth, but not parental employment status. We found cross-national and regional variation in associations between SES and food habits and differences in the extent to which the respective indicators of SES were related to children's diet. Conclusion: Socioeconomic differences in children's food habits exist in the majority of European and Asian countries examined in this study. The results are of relevance when addressing strategies, policy actions, and interventions targeting social inequalities in children's diets.
- Development of Self-disinfecting Paints and In Vitro Assessment of their Cytotoxicity on Human CellsPublication . Querido, Micaela; Paulo, Ivo; Galhano Santos, Rui; Bordado, João; Teixeira, João Paulo; Pereira, CristianaThis PhD project aims to develop, test and validate a self-disinfecting paint, to be applied in areas with a high propensity for infection spreading, such as healthcare facilities, schools or other public spaces, to help reduce infection risks.
- Subcutaneous phaeohyphomycosis due to Phaeoacremonium parasiticum in a patient with Chronic Granulomatous DiseasePublication . Carvalho, Dinah; Sabino, Raquel; Veríssimo, Cristina; Simões, Helena; Lopes Silva, Susana; Marques, Tiago; Janeiro, João; Marques Lito, Luis; Melo Cristino, JoséObjectives: Phaeoacremonium parasiticum is a ubiquitous dematiaceous mold that rarely causes infection in humans. Its spectrum of disease ranges from subcutaneous infections to disseminated disease. The majority of those reported few cases involve immunocompromised patients. Chronic granulomatous disease (CGD) is an inherited disorder affecting nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex. CGD patients are susceptible to a broad spectrum of opportunistic infections, being fungal infections a major determinant of survival. We report a case of Subcutaneous phaeohyphomycosis due to P. parasiticum in a young adult with CGD. Material and methods: A 28-year-old male patient with autossomic recessive CGD, due to mutations in CYBA, is followed in Primary Immunodeficiency Center since childhood. He has been under prophylaxis with itraconazole, cotrimoxazole and IFNg and tapering oral steroids, started for granulomatous colitits 2 years earlier. In a regular visit to the clinical center, he complained of persistent pain on his left leg with no history of recent injury in the affected area and without external inflammatory signs. An ultrasound was performed after 2 weeks revealing a heterogeneous liquid of slightly irregular contour in the sinus of the left anterior tibial muscle with heterogeneity of the adjacent muscle. This piomiositis collection was punctured, under ultrasound control, with drainage of about 7cm3 of hemato-purulent exsudate that was promptly processed for bacteriology (aerobic and anaerobic) and mycology studies. Results: Cultures for bacteria were sterile. Culture on Sabouraud was positive after 5 days of incubation, showing slow growing and initially white colonies. Microscopic examination showed hyaline, septate mycelium with long, thin conidiophores and curved, aseptate conidia.The fungus was initially identified as an Acremonium sp. and was sent to the Mycology Reference Laboratory for molecular identification and antifungal susceptibility testing. Its identification was performed by sequencing the internal transcribed spacer (ITS) region of ribosomal DNA, being the isolate identified as Phaeoacremonium parasiticum (100% homology). After 3 weeks incubation, coloration of the colonies emerged, becoming greyish black upon subculture, with velvety texture and black reverse. Microscopically, pigmented hyphae with tapering, funnel-shaped phialides were observed, and conidia were hyaline and oblong, forming clusters at the tip of the phialides. At this point, macroscopic and microscopic morphology was consistent with Phaeoacremonium species. Susceptibility pattern showed low minimal inhibitory concentrations (MIC) to posaconazole and voriconazole, and higher MIC values to anidulafungin and amphotericin B. The patient has improved under voriconazol therapy (200 mg; bid) Conclusions: Phaeoacremonium parasiticum is an uncommon infection and its appropriate identification is often difficult because morphologically, the genus Phaeoacremonium show morphological features resembling both Acremonium and Phialophora genera. Molecular identification is determinant to confirm morphology, as many species have indistinguishable characteristics that may lead to incorrect antifungal options. Also, susceptibility testing should be done for these so rare fungi as optimal treatment has not yet been clearly defined.
- Maternal tobacco smoke exposure and determination of the optimal urinary cotinine cut-off values: a cross-sectional birth studyPublication . Silva, AI; Camelo, Alexandra; Madureira, Joana; Reis, Ana Teresa; Barbosa, Fernando Jr; Teixeira, J,P,; Costa, CarlaWithin the frame of the NEOGENE project, the aim of this study was to identify the optimal UC cut-off values to discriminate smoking and ETS exposure status and to assess the validity of self-reported smoking and ETS exposure status using urinary cotinine (UC) as a biomarker.
- Relatório de Atividades 2020Publication . Instituto Nacional de Saúde Doutor Ricardo Jorge, IP; Rama, Patrícia; Costa, SofiaO presente instrumento de gestão do Instituto Nacional de Saúde Doutor Ricardo Jorge (INSA) relativo ao ano de 2020 tem três grandes objetivos: 1) Averiguar a concretização do Quadro de Avaliação e Responsabilização (QUAR) e do Plano de Atividades (PA) de 2020, nomeadamente, avaliar a estratégia assumida através dos seus objetivos estratégicos, verificando o grau de realização dos programas e dos objetivos operacionais que foram propostos nestes documentos; 2) Disponibilizar informação institucional relativa à execução do orçamento, à alocação de recursos humanos, financeiros e técnicos; 3) Demonstrar quantitativa, e qualitativamente, a informação relativa às funções essenciais do Instituto, aos Departamentos Técnico Científicos (DTC), ao Museu da Saúde e aos Serviços de Suporte.
- A importância dos artrópodes ixodídeos para a Saúde Pública na Região de Saúde do CentroPublication . Torres, C.; Santos, A.S.; Lopes de Carvalho, I.; Gomes, F.; Serrada, E.; Gonçalo, J.; Codeiro, E.; Menezes de Almeida, L.; Tavares, A.; Santos Silva, M.; Núncio, M.S.; Pimentel, J.P.As doenças transmitidas por vetores têm reemergido → alterações climáticas, demográficas, genéticas nos agentes patogénicos, resistências a inseticidas e mobilidade de populações e bens. Os ixodídeos são um dos vetores com mais impacto na Saúde Pública. Este estudo pretende caracterizar a distribuição de ixodídeos por critérios temporo-espaciais, ambientais e parasitismo, no âmbito da Rede de Vigilância de Vetores (REVIVE), da Administração Regional de Saúde do Centro (ARSC) de 2015 a 2019.
- Wide spectrum of F9 variants in hemophilia B families from the Portuguese populationPublication . Moreira, Isabel; Diniz, Maria João; Tavares, Alice; Morais, Sara; Freitas, B.; Araújo, F.; Gago, T.; Antunes, EM; Catarino, C.; Campos, M.; Almeida, T.; Santos, S.B.; Maria, R.; Kjollerstrom, P.; Lavinha, João; David, DezsoIntroduction: Hemophilia B is an X-linked bleeding disorder caused by molecular defects in the Factor IX gene (F9), leading to either deficiency or functional abnormality of Factor IX. Actual data indicate a high heterogeneity of variants in F9. Over 1000 different variants have been reported, including pathogenic single nucleotide variants (SNPs), indels and complex variants. Materials and Methods: 86 index patients and 313 relatives were studied. F9 variant analysis was performed from total genomic DNA by PCR followed either by SSCP and DNA sequencing or direct DNA sequencing. When no variant was detected by sequencing, F9 analysis by MLPA was performed. Segregation studies were performed in each family. Results: Overall, 52 different F9 variants have been identified, including 49 SNPs or small indels, a gross duplication (exons 2-6) and two deletions of the entire gene. Ten of the variants had been firstly reported by us and three are novel: c.391+5G>T; c.432T>G, p.(Phe144Leu) and c.749C>A, p.(Ala250Glu). This approach allowed establishing the carrier state of over 300 women and 12 prenatal diagnoses were performed. Conclusions: The spectrum of F9 variants identified in the Portuguese population significantly overlaps that observed in other populations. Identification of F9 gene variants in patients allows genotype-phenotype correlations and carrier detection, as well as prenatal diagnosis. Sanger sequencing of the coding region and adjacent intronic sequences of F9 still remains a valid and effective tool for the molecular study of hemophila B, providing information for appropriate genetic counseling and new insights regarding the molecular basis of the pathology.
- Eumicetoma causado por Parathyridaria percutanea num doente imunodeprimido - caso importadoPublication . Fraga, Marta; Carvalho, Dinah; Sabino, Raquel; Veríssimo, Cristina; Simões, Helena; Sapinho, Guilherme; Alho4, Ana; Espada, Eduardo; Moreno, Raul; Forjaz de Lacerda, J.; Lito, L. Marques; Cristino, J. MeloEumicetoma é uma infeção fúngica subcutânea crónica caracterizada por edema, fistulização e libertação de grânulos. Parathyridaria percutanea é uma causa rara de feohifomicose subcutânea, não havendo casos descritos de formação de fistulas ou drenagem de grãos devido a este fungo. Apresenta-se um caso clínico de eumicetoma causado por Parathyridaria percutanea
- Contributos da dieta vegetariana para o aporte de mineraisPublication . Santiago, Susana; Bárbara, RitaA dieta vegetariana é um padrão alimentar de base vegetal, caraterizado pela abstenção de consumo de produtos de origem animal, maioritariamente carne, peixe e marisco (1). A dieta vegetariana apresenta diversas vertentes, algumas incluem o consumo de ovos e/ou leite e derivados, como a ovolactovegetariana e outras, mais restritivas, como a vegan, que excluem a ingestão de qualquer produto de origem animal (2). (...)