Browsing by Author "Rodrigues, Joana"
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- Food additives intake assessment – a school population case studyPublication . Rodrigues, Joana; Calmeiro, Ana; Vasco, ElsaIntroduction/Aim: Food additives are safe, however consumption of some of them should not exceed the Acceptable Daily Intake (ADI). In this regard, European Commission requires member states to monitor the intake of these additives at national level. When there are no national food consumption data available, the evaluation can initially be performed in population subgroups of higher risk, such as young people. Additives intake assessment studies are carried out, in a first approach, by crossing the food consumption data with the maximum permitted levels of additives in food (LMU), to identify the additives which intake could constitute a health concern. OPEN Portugal is an online nutritional platform that allows the evaluation of food consumption. The aim of this study was to evaluate the use of the OPEN Portugal tool in obtaining food consumption data by a school population, to identify most consumed food additives and those that need more detailed and refined studies
- Genome-scale analysis of Mycobacterium avium complex isolates from Portugal reveals extensive genetic diversityPublication . Carneiro, Sofia; Pinto, Miguel; Rodrigues, Joana; Gomes, João Paulo; Macedo, RitaOpportunist infections caused by nontuberculous mycobacteria (NTM) have emerged as a significant public health problem. Among these, species of the Mycobacterium avium complex (MAC) are the main responsible for the increase in the number of human disease cases. In order to address the current needs in the detection and surveillance of MAC disease cases, we evaluated different species classification methodologies (BLASTn-based marker-gene approach, Kraken v2, rMLST and MLST databases) and their congruence with a core-SNP phylogenetic approach, based on whole genome sequencing (WGS) data. For this purpose, we used a collection of 142 MAC isolates from Portuguese patients diagnosed between 2014 and 2022. The marker-gene approach (based on the rpoB, hsp65 and groEL genes), showed the best results, allowing the identification of the 142 MAC isolates to the species/subspecies level (M. avium subsp. hominissuis, M. intracellulare, M. intracellulare subsp. chimaera, M. intracellulare subsp. yongonense, M. marseillence and M. colombiense). Additionally, we performed drug susceptibility testing that confirmed clarithromycin efficacy as a first-line treatment for MAC disease, as 93 % of the Portuguese isolates were susceptible. Using a core-SNP approach we also performed an in-depth phylogenetic analysis within each identified species group, and despite the high genetic diversity within the MAC species, we were able to clearly distinguish all the species/subspecies and identify genetic clusters with epidemiological potential. We highlight not only the need for the standardization of an appropriate genotyping approach for species identification and management of MAC disease, but also a more robust large-scale WGS data analysis, in a One Health perspective, in order to identify potential routes of transmission.
- A OPEN Portugal na avaliação do consumo de Aditivos Alimentares: estudo de casoPublication . Rodrigues, Joana; Calmeiro, Ana; Oliveira, Luísa; Vasco, ElsaApresentação do trabalho "A OPEN Portugal na avaliação do consumo de Aditivos Alimentares" selecionado de entre os abstracts submetidos no âmbito da reunião anual do PortFIR - Portal de Informação Alimentar (http://portfir.insa.pt/). O PortFIR é um programa de implementação de redes de excelência Portuguesas em nutrição e segurança alimentar e um portal que inclui bases de dados sustentáveis e de qualidade reconhecida sobre Composição e Contaminação de Alimentos e Consumos Alimentares.
- A OPEN Portugal na avaliação do consumo de Aditivos Alimentares: estudo de casoPublication . Rodrigues, Joana; Calmeiro, Ana; Oliveira, Luísa; Vasco, ElsaIntrodução: Entende-se por aditivo alimentar toda a substância adicionada de forma intencional a um alimento com o objetivo de melhorar as suas características originais. A Comissão Europeia exige a realização pelos estados membros da monitorização da ingestão destes aditivos a nível nacional. No entanto, quando não existem dados de consumo alimentar nacional a mesma pode, inicialmente, ser feita em grupos populacionais de maior risco, como é o caso dos jovens. A OPEN Portugal é uma plataforma nutricional on-line que permite avaliar o consumo alimentar através do preenchimento de um diário alimentar. Com esta plataforma é possível obter dados de consumo alimentar, de energia e do estado nutricional do indivíduo, de acordo com a tabela de composição de alimentos Portuguesa. Para avaliar o consumo diário de aditivos alimentares pelos indivíduos é calculada a ingestão diária estimada (IDE), a partir da relação entre os limites máximos de utilização do aditivo no alimento (LMU), o consumo do alimento pelo indivíduo (x) e o peso corporal do indivíduo (pc). A IDE para um determinado aditivo alimentar é então comparada com a dose diária admissível (DDA).