Browsing by Author "Martins, Fátima"
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- Alterações no consumo alimentar e outros comportamentos relacionados com a alimentação em crianças durante o confinamento em contexto da pandemia da COVID-19, em Portugal: programa MUN-SI Cascais 2019/2020Publication . Baleia, Joana; Pirata, Catarina; Mendes, Sofia; Figueira, Inês; Martins, Fátima; Rito, Ana IsabelO confinamento obrigatório e o encerramento das escolas durante a pandemia da COVID-19 afetaram as rotinas e estilos de vida das crianças, com impacto na sua saúde. Este trabalho pretende identificar as mudanças no consumo e comportamentos alimentares durante o primeiro confinamento em contexto da pandemia da COVID-19 em 2020, entre crianças do 1.º Ciclo do Ensino Básico (EB). Este estudo transversal decorreu dois meses após o início do confinamento, em 11 escolas participantes no programa MUN-SI Cascais, abrangendo crianças entre os 8-10 anos. Os dados foram recolhidos através de um questionário de família online, no qual 113 encarregados de educação (EE) participaram voluntariamente. Os EE reportaram alterações na ingestão alimentar das crianças durante o confinamento como o aumento da ingestão de cereais de pequeno-almoço, pão de forma embalado e batatas fritas (25,5%, 19,4% e 18,8%, respetivamente). Metade dos EE (50,0%) reportaram ter comprado mais produtos locais, 22,4% compraram mais produtos em grandes quantidades e 19,7% usaram mais apps/lojas online para adquirir alimentos durante o confinamento. Destacam-se ainda outros comportamentos que aumentaram durante o confinamento: prestar mais atenção aos prazos de validade (52,1%), fazer mais listas de compras (51,4%), comer em horários mais regulares (43,2%), prestar mais atenção ao desperdício de alimentos (35,7%) e maior envolvimento das crianças na preparação das refeições (27,8%). Estes dados reforçam a necessidade de manter e incentivar estratégias de desenvolvimento de políticas efetivas em saúde, de base escolar e familiar, durante e após o período pós-pandemia.
- Comparison of immunohematological profile between endurance- and power-oriented elite athletesPublication . Morgado, José P.; Matias, Catarina N.; Monteiro, Cristina P.; Alves, Francisco; Reis, Joana F.; Santos, Diana A.; Silva, Analiza M.; Martins, Fátima; Seixas, Maria T.; Rocha-Pereira, Petronila; Sardinha, Luís B.; Laires, Maria J.There is general perception that elite athletes are highly susceptible to changes in immunohematological profile. The objective of this study was to compare immunohematological parameters of elite athletes of different aerobic and muscular strength sports and analyze changes over 2 months. Sixteen judoists and 14 swimmers were evaluated 2 months before (M1) and immediately prior to competition (M2). Hemogram and lymphocytes subpopulations were assessed with automatic counter and flow cytometry, respectively. Judoists had higher neutrophils and lower monocytes and eosinophils percentages than swimmers at M1 and M2. At M2 judoists had lower red blood cells (RBC), hemoglobin, and hematocrit than swimmers. At M2 judoists' hematocrit and CD16 decreased while swimmers' hemoglobin and hematocrit increased. In conclusion, neither sports characteristics nor intense training seem to displace the athletes' immunohematological profile out of the clinical range, despite the possibility of occurrence of microlesions that may stimulate production of leukocytes and reduction of RBC in judoists.
- Facts related to the collection of biological samples in the National Health Examination Survey - Portuguese Component of the European Health Examination SurveyPublication . Silva, Marta Barreto da; Francisco, Vânia; Rasteiro, Paula; Sousa, Eduardo; Vicente, A.M.; Bourbon, Mafalda; Martins, Fátima; Seixas, Maria Teresa; Fernandes, Aida; Beleza, Álvaro; Mendonça, Francisco; Gil, Ana Paula; Dias, Carlos MatiasThe objective of the National Health Examination Survey (NHES), which corresponds to the Portuguese component of the European Health Examination Survey (EHES), is to collect health data, related risk factors and biological samples of the Portuguese population, using the EHES recommended methodology. These surveys involve an interview, clinical and physical measurements and blood collection. In this context, we herein describe the pilot study performed in S. Brás de Alportel in the Algarve region. For this pilot study, we have recruited 221 individuals (95 males and 126 females), between 25 and 91 years old, who were enrolled in the Health Centre of S. Brás de Alportel (Algarve). For each participant, we have collected 16.5 ml of total blood, in five different Vacutainer® tubes, which was later processed into serum, plasma and DNA. We have performed several biochemical analyses(total cholesterol, LDL,HDL, glucose, tryglicerides, creatinine, ALT, AST, -GT, CRP and iron) and a complete blood count. From the 221 participants in this pilot study, we were able to collect blood to 219 (99.5%). To 185 of these (84.5%) we were able to collect the total amount of blood. The biochemical analyses were performed in all the samples. The total blood count was performed in 103 samples (47%) due to transport constraints. We have also collected DNA from 210 participants (95.9%). We have created a biobank comprising 1847 serum aliquots and 959 plasma aliquots, which have been stored at - 80°C and 210 DNA aliquots which have been stored at 4°C. In conclusion, during this study, we have optimized the logistics and procedures to perform the large scale study for the NHES and EHES. In addition, we have created a biobank comprising detailed questionnaire data, physical and clinical data and biological samples from a representative sample of S. Brás de Alportel in Algarve, Portugal. This biobank will allow us to perform future studies, including the determination of the prevalence of gene variants of public health interest, the characterization of gene-environment interactions in the development of chronic diseases and the genetic structure of the Portuguese population. The success rate, the quality of the data and of the biological samples was high and comparable to similar studies.
- Portuguese National Serological Survey to Coronavirus Disease 19 - ISNCOVID-19Publication . Kislaya, Irina; Gómez, Verónica; Garcia, Ana Cristina; Machado, Ausenda; Torres, Ana Rita; Roquette, Rita; Sousa-Uva, Mafalda; Matias Dias, Carlos; Nunes, Baltazar; Costa, Inês; Gonçalves, Paulo; Matos, Rita; Machado, Jorge; Manita, Carla; Martins, Fátima; Santos, João; de Sousa, Rita; Soeiro, Sofia; Rocha, Raquel; Roque, Carla; Verdasca, Nuno; Guiomar, Raquel; Rodrigues, Ana Paula; ISNCOVID-19 study teamThe objective of this seroepidemiological survey was to estimate seroprevalence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) specific antibodies (IgM and/or IgG) in Portugal in May-July 2020. The seroprevalence of SARS-Cov-2 infection (positive for IgM and/or IgG) was 2.9% (CI95: 2.0 - 4.2%). Our results support extend limited extent of infection by SARS-CoV-2 in Portugal due to early lockdown measures implementation.
- Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in PortugalPublication . Borges, Vítor; Duque, Mariana Perez; Martins, João Vieira; Vasconcelos, Paula; Ferreira, Rita; Sobral, Daniel; Pelerito, Ana; de Carvalho, Isabel Lopes; Núncio, Maria Sofia; Borrego, Maria José; Roemer, Cornelius; Neher, Richard A.; O’Driscoll, Megan; Rocha, Raquel; Lopo, Sílvia; Neves, Raquel; Palminha, Paula; Coelho, Luís; Nunes, Alexandra; Isidro, Joana; Pinto, Miguel; Santos, João Dourado; Mixão, Verónica; Santos, Daniela; Duarte, Silvia; Vieira, Luís; Martins, Fátima; Machado, Jorge; Veríssimo, Vítor Cabral; Grau, Berta; Peralta-Santos, André; Neves, José; Caldeira, Margarida; Pestana, Mafalda; Fernandes, Cândida; Caria, João; Pinto, Raquel; Póvoas, Diana; Maltez, Fernando; Sá, Ana Isabel; Salvador, Mafalda Brito; Teófilo, Eugénio; Rocha, Miguel; Moneti, Virginia; Duque, Luis Miguel; e Silva, Francisco Ferreira; Baptista, Teresa; Vasconcelos, Joana; Casanova, Sara; Mansinho, Kamal; Alves, João Vaz; Alves, João; Silva, António; Alpalhão, Miguel; Brazão, Cláudia; Sousa, Diogo; Filipe, Paulo; Pacheco, Patrícia; Peruzzu, Francesca; de Jesus, Rita Patrocínio; Ferreira, Luís; Mendez, Josefina; Jordão, Sofia; Duarte, Frederico; Gonçalves, Maria João; Pena, Eduarda; Silva, Claúdio Nunes; Guimarães, André Rodrigues; Tavares, Margarida; Freitas, Graça; Cordeiro, Rita; Gomes, João PauloPathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the highest impact on case numbers and fitted them into a global context, finding evidence that several international sublineages probably emerged or spread early in Portugal. We estimated a 62% infection reporting rate and that 1.3% of the population of men who have sex with men in Portugal were infected. We infer the critical role played by sexual networks and superspreader gatherings, such as sauna attendance, in the dissemination of mpox virus. Overall, our findings highlight genomic epidemiology as a tool for the real-time monitoring and control of mpox epidemics, and can guide future vaccine policy in a highly susceptible population.