Browsing by Author "Clarke, Luka"
Now showing 1 - 4 of 4
Results Per Page
Sort Options
- Molecular regulation of the plasma membrane retention of disease-related chloride channels CFTR and NKCC2Publication . Loureiro, Claudia; Matos, P; Clarke, Luka; Jordan, PeterThree disease-related chloride transport proteins, CFTR (in cystic fibrosis (CF) or chronic obstructive pulmonary disease (COPD)), NKCC2 and KCC3 (in kidney function and hypertension), were found to specific targets for protein kinase Syk which phosphorylates a specific tyrosine residue in each channel. Tyrosine phosphorylation downregulates the amount of CFTR [1] present at the plasma membrane and a better understanding of this process may reveal novel therapeutic options for CF patients. Thus, we determined the cellular adaptor proteins able to recognize the phospho-tyrosine modification and mediate traffic to the plasma membrane.
- New insights into how cancer cells regulate glucose uptake by protein phosphorylationPublication . Henriques, Andreia; Matos, Paulo; Clarke, Luka; Jordan, PeterCancer cells require increased amounts of glucose to sustain their proliferation and upregulate plasma membrane expression of glucose transporter GLUT1. In insulin responsive cells, glucose uptake requires previous phosphorylation of TBC1D4, a negative regulator of endosomal GLUT traffic. Previous work published by the host lab has discovered that protein kinase WNK1 can also phosphorylate TBC1D4 and promote the translocation of GLUT1 to the cell surface. In vitro, WNK1 also phosphorylates the homologue TBC1D1 for which a role in cancer cell glucose uptake is not known.
- Regulation of Glucose uptake in mammalian cells by phosphorylation networksPublication . Henriques, Andreia; Jordan, Peter; Clarke, LukaObjectives: Characterize the role of protein kinase WNK1 in the phosphorylation network regulating cellular glucose uptake
- The adaptor protein SHC1 is recruited to tyrosine-phosphorylated plasma membrane chloride channelsPublication . Loureiro, Claudia; Matos, Paulo; Clarke, Luka; Jordan, PeterProtein kinase Syk was recently found to phosphorylate a specific tyrosine residue in three distinct disease-related chloride transport proteins: CFTR (in cystic fibrosis (CF) or chronic obstructive pulmonary disease (COPD)), NKCC2 and KCC3 (in kidney function and hypertension).