Browsing by Author "Canabarro, Paula Lima"
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- Aspergillosis in free-ranging Magellanic penguinsPublication . Melo, Aryse Martins; Poester, Vanice Rodrigues; Trápaga, Mariana Rodrigues; Stevens, David A.; Canabarro, Paula Lima; Adornes, Andréa Corrado; da Silva, Andrine Paiva; Estima, Sérgio Curi; Frere, Esteban; Sabino, Raquel; Xavier, Melissa OrzechowskiWe evaluated the mortality due to aspergillosis in free-ranging Magellanic penguins during their migration and the reproductive season. A total of 98 carcasses of penguins were collected along 370 km of coastline in Southern Brazil, between June 2017 and October 2019, and from reproductive colonies in Patagonian Argentina, in January 2019. All animals were necropsied, and only proven cases were computed. Aspergillosis was diagnosed in 2.5% of the penguins evaluated during their migration route. Our study, of the Southern coast of Brazil, is the first to demonstrate that aspergillosis is an important cause of mortality in free-ranging penguins. The implications of these findings in the One Health context are discussed
- Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolatesPublication . Melo, Aryse Martins; Poester, Vanice Rodrigues; Canabarro, Paula Lima; Sampaio, Daniel Ataíde; Stevens, David A; Veríssimo, Cristina; Sabino, Raquel; Xavier, Melissa OrzechowskiAspergillus section Fumigati is reported in up to 99% of aspergillosis cases in penguins. So far, no data regarding molecular epidemiology and azole resistance are available for A. fumigatus isolates collected from Magellanic penguins. The aim of this work was to perform molecular identification of Aspergillus section Fumigati at species level, to genotype those isolates using microsatellite markers, to evaluate the in vitro susceptibility patterns of A. fumigatus sensu stricto, and to characterize the cyp51A gene in clinical A. fumigatus strains isolated from Magellanic penguins with proven aspergillosis. All 34 isolates included in the study were identified as A. fumigatus sensu stricto. Analyzing the genetic diversity of the isolates of A. fumigatus sensu stricto, we identified two possible outbreaks in the rehabilitation center and we also observed the maintenance of clonal strains through the years. One A. fumigatus sensu stricto isolate was resistant to posaconazole, but the mutations found in the cyp51A gene of this isolate have not been described as conferring phenotypic resistance, suggesting that other mechanisms of resistance could be involved in the resistance of this isolate. With this study, we were able to understand the molecular diversity of Aspergillus fumigatus isolates collected from Magellanic penguins, to characterize them and to associate them with the described global population of Aspergillus fumigatus.