Loading...
Publication
Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolates
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 965.49 KB | Adobe PDF |
Advisor(s)
Abstract(s)
Aspergillus section Fumigati is reported in up to 99% of aspergillosis cases in penguins. So far, no data regarding
molecular epidemiology and azole resistance are available for A. fumigatus isolates collected from
Magellanic penguins. The aim of this work was to perform molecular identification of Aspergillus section
Fumigati at species level, to genotype those isolates using microsatellite markers, to evaluate the in vitro
susceptibility patterns of A. fumigatus sensu stricto, and to characterize the cyp51A gene in clinical A. fumigatus
strains isolated from Magellanic penguins with proven aspergillosis. All 34 isolates included in the
study were identified as A. fumigatus sensu stricto. Analyzing the genetic diversity of the isolates of A. fumigatus
sensu stricto, we identified two possible outbreaks in the rehabilitation center and we also observed
the maintenance of clonal strains through the years. One A. fumigatus sensu stricto isolate was resistant
to posaconazole, but the mutations found in the cyp51A gene of this isolate have not been described as
conferring phenotypic resistance, suggesting that other mechanisms of resistance could be involved in the
resistance of this isolate. With this study, we were able to understand the molecular diversity of Aspergillus
fumigatus isolates collected from Magellanic penguins, to characterize them and to associate them with the
described global population of Aspergillus fumigatus.
Plain Language Summary: A. fumigatus sensu stricto is of great importance in penguins’ aspergillosis. We could identify two outbreaks in the rehabilitation center and the maintenance of clonal strains through the years. Regarding antifungal prophylaxis, it may proceed, but preferably with surveillance for azole resistance.
Plain Language Summary: A. fumigatus sensu stricto is of great importance in penguins’ aspergillosis. We could identify two outbreaks in the rehabilitation center and the maintenance of clonal strains through the years. Regarding antifungal prophylaxis, it may proceed, but preferably with surveillance for azole resistance.
Description
Keywords
Molecular Identification Penguins Mechanisms of Resistance Aspergillus Resistance Microsatellite Markers Genomic Diversity Genotyping Infecções Sistémicas e Zoonoses
Pedagogical Context
Citation
Med Mycol. 2021 Nov 3;59(11):1076-1084. doi: 10.1093/mmy/myab040
Publisher
Oxford University Press/ International Society for Human and Animal Mycology