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Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolates

2021-11-03Artigo científico Acesso restrito
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dc.contributor.authorMelo, Aryse Martins
dc.contributor.authorPoester, Vanice Rodrigues
dc.contributor.authorCanabarro, Paula Lima
dc.contributor.authorSampaio, Daniel Ataíde
dc.contributor.authorStevens, David A
dc.contributor.authorVeríssimo, Cristina
dc.contributor.authorSabino, Raquel
dc.contributor.authorXavier, Melissa Orzechowski
dc.date.accessioned2021-12-23T16:15:13Z
dc.date.available2021-12-23T16:15:13Z
dc.date.issued2021-11-03
dc.description.abstractAspergillus section Fumigati is reported in up to 99% of aspergillosis cases in penguins. So far, no data regarding molecular epidemiology and azole resistance are available for A. fumigatus isolates collected from Magellanic penguins. The aim of this work was to perform molecular identification of Aspergillus section Fumigati at species level, to genotype those isolates using microsatellite markers, to evaluate the in vitro susceptibility patterns of A. fumigatus sensu stricto, and to characterize the cyp51A gene in clinical A. fumigatus strains isolated from Magellanic penguins with proven aspergillosis. All 34 isolates included in the study were identified as A. fumigatus sensu stricto. Analyzing the genetic diversity of the isolates of A. fumigatus sensu stricto, we identified two possible outbreaks in the rehabilitation center and we also observed the maintenance of clonal strains through the years. One A. fumigatus sensu stricto isolate was resistant to posaconazole, but the mutations found in the cyp51A gene of this isolate have not been described as conferring phenotypic resistance, suggesting that other mechanisms of resistance could be involved in the resistance of this isolate. With this study, we were able to understand the molecular diversity of Aspergillus fumigatus isolates collected from Magellanic penguins, to characterize them and to associate them with the described global population of Aspergillus fumigatus.pt_PT
dc.description.abstractPlain Language Summary: A. fumigatus sensu stricto is of great importance in penguins’ aspergillosis. We could identify two outbreaks in the rehabilitation center and the maintenance of clonal strains through the years. Regarding antifungal prophylaxis, it may proceed, but preferably with surveillance for azole resistance.pt_PT
dc.description.sponsorshipThis study was supported by the International Cooperation Program financed by the Brazilian Federal Agency for Support and Evaluation of Graduate Education within the Ministry of Education of Brazil (CAPES), under the PDSE Program - Finance Code 001.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMed Mycol. 2021 Nov 3;59(11):1076-1084. doi: 10.1093/mmy/myab040pt_PT
dc.identifier.doi10.1093/mmy/myab040pt_PT
dc.identifier.issn1369-3786
dc.identifier.urihttp://hdl.handle.net/10400.18/7831
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Press/ International Society for Human and Animal Mycologypt_PT
dc.relation.publisherversionhttps://academic.oup.com/mmy/article-abstract/59/11/1076/6329687?redirectedFrom=fulltextpt_PT
dc.subjectMolecular Identificationpt_PT
dc.subjectPenguinspt_PT
dc.subjectMechanisms of Resistancept_PT
dc.subjectAspergillus Resistancept_PT
dc.subjectMicrosatellite Markerspt_PT
dc.subjectGenomic Diversitypt_PT
dc.subjectGenotypingpt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleMolecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolatespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1084pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage1076pt_PT
oaire.citation.titleMedical Mycologypt_PT
oaire.citation.volume59pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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