Percorrer por autor "Brondani, Greice"
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- Airborne spores’ dissemination of a swine associated Clostridioides difficile clonePublication . Alves, Frederico; Cano, Manuela; Brondani, Greice; Nunes, Alexandra; Oleastro, MónicaThis study supports the airborne dissemination of Clostridioides difficile spores. Of the sieve impaction samples collected at a swine production unit, 66.7% were positive and all belonged to the predominantly established clone. Spores' density varied according to the characteristics of the animal population, suggesting the possibility of airborne transmission.
- An Overview of Monkeypox Virus Detection in Different Clinical Samples and Analysis of Temporal Viral Load DynamicsPublication . Cordeiro, Rita; Pelerito, Ana; de Carvalho, Isabel Lopes; Lopo, Sílvia; Neves, Raquel; Rocha, Raquel; Palminha, Paula; Verdasca, Nuno; Palhinhas, Cláudia; Borrego, Maria José; Manita, Carla; Ferreira, Idalina; Bettencourt, Célia; Vieira, Patrícia; Silva, Sónia; Água-Doce, Ivone; Roque, Carla; Cordeiro, Dora; Brondani, Greice; Santos, João Almeida; Martins, Susana; Rodrigues, Irene; Ribeiro, Carlos; Núncio, Maria Sofia; Gomes, João Paulo; Batista, Fernando da ConceiçãoMpox is a zoonotic disease caused by the Monkeypox virus (MPXV), and since May 2022, tens of thousands of cases have been reported in non-endemic countries. We aimed to evaluate the suitability of different sample types for mpox diagnostic and assess the temporal dynamics of viral load. We evaluated 1914 samples from 953 laboratory-confirmed cases. The positivity rate was higher for lesion (91.3%) and rectal swabs (86.1%) when compared with oropharyngeal swabs (69.5%) and urines (41.2%), indicating higher viral loads for the former. Supporting this, lesion and rectal swabs showed lower median PCR C values (C = 23 and C = 24), compared to oropharyngeal swabs and urines (C = 31). Stable MPXV loads were observed in swabs from lesions up to 30 days after symptoms onset, contrasting with a considerable decrease in viral load in rectal and oropharyngeal swabs. Overall, these results point to lesion swabs as the most suitable samples for detecting MPXV in the 2022-2023 multicountry outbreak and show comparable accuracy to rectal swabs up to 8 days after symptoms onset. These findings, together with the observation that about 5% of patients were diagnosed through oropharyngeal swabs while having negative lesions, suggest that multisite testing should be performed to increase diagnostic sensitivity.
- Viruses in Beach SandPublication . Santos, Ricardo; Brandão, João; Brondani, Greice; Monteiro, SílviaBeach sand is a broad habitat inhabited by many organisms. The organisms inhabiting supratidal and intertidal sands and the ones found above the margin of an aquifer are termed psammon. The most commonly known is possibly the macropsammon comprised of molluscs, crustaceans, and annelids. The meiopsammon, less familiar, are near-microscopic organisms such as nematodes, copepods, and flatworms often predators, collectors and glazers. Even less studied are the sand microbes also termed micropsammon. Remarkably, little is known about the micropsammon especially in comparison to soil, water, and bottom/submerged sediment. Sand microbes include autochthonous species and allochthonous organisms, including faecal indicator bacteria (FIB) and other waterborne pathogens such as viruses and protozoa, which are deposited by way of waves, runoff, air, and animals. The destiny of these microorganisms spans from death, to temporary persistence and/or possible replication, to naturalization of thriving populations and integration in the indigenous community. Undoubtedly, fungi and bacteria are able to proliferate in sand. The detection of culturable fungi and direct microscopic bacterial counts from sand has been previously reported, with numbers up to 1.5 - 7.6 x106 CFU/g and greater than 107/g sand, respectively. Transport of the micropsammon occurs vertically, from ground water to sand surface and horizontally across the beach. Additionally, transport through interstitial flow, particle-associated microbes, and terrestrial runoff and wave movement are also possible. A multiplicity of pathogens has been studied from beach sand with recent epidemiological reports showing some correlation between sand exposure and associated health risks. Only a few studies have been conducted on the occurrence of enteric viruses in beach sand. These studies focused mainly on the detection of cultivable enteric viruses, i.e. enteroviruses and reoviruses. No studies have focused on the presence of noroviruses and hepatitis A viruses, the main aethiological agents of water and foodborne gastroenteritis and hepatitis. The same happens for the presence of hepatitis E viruses. The present study aimed to detect different pathogenic viruses: noroviruses GI (NoVGI), noroviruses GII (NoVGII), hepatitis A (HAV) and hepatitis E (HEV), Adenovirus (HadV) and JC polyomavirus in beach sand. HAV were detected in 6.25% (3 out of 48) and HadV were detected in 8.33% (4 out of 48) samples using qPCR. These results corroborate the reporting of sand as an independent source of GI illness. The present study emphasizes the need to include sand in regulation and analytical standards for microbiological quality of bathing beaches. The assessment of water quality by itself is not adequate for protecting public health of recreational users, as previously demonstrated and now confirmed by our results. Moreover, the persistence and presence of viruses associated with human disease does not correlated with the current FIB.