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Proteome variability among Helicobacter pylori isolates clustered according to genomic methylation

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dc.contributor.authorVitoriano, Inês
dc.contributor.authorVítor, Jorge
dc.contributor.authorOleastro, Mónica
dc.contributor.authorRoxo-Rosa, Mónica
dc.contributor.authorVale, Filipa
dc.date.accessioned2013-07-29T15:39:59Z
dc.date.available2013-07-29T15:39:59Z
dc.date.issued2013-06
dc.description.abstractAims: To understand whether the variability found in the proteome of Helicobacter pylori relates to the genomic methylation, virulence and associated gastric disease. Methods and Results: We applied the Minimum-Common-Restriction- Modification (MCRM) algorithm to genomic methylation data of 30 Portuguese H. pylori strains, obtained by genome sensitivity to Type II restriction enzymes’ digestion. All the generated dendrograms presented three clusters with no association with gastric disease. Comparative analysis of twodimensional gel electrophoresis (2DE) maps obtained for total protein extracts of 10 of these strains, representative of the three main clusters, revealed that among 70 matched protein spots (in a universe of 300), 16 were differently abundant (P < 0 05) among clusters. Of these, 13 proteins appear to be related to the cagA genotype or gastric disease. The abundance of three protein species, DnaK, GlnA and HylB, appeared to be dictated by the methylation status of their gene promoter. Conclusions: Variations in the proteome profile of strains with common geographic origin appear to be related to differences in cagA genotype or gastric disease, rather than to clusters organized according to strain genomic methylation. Significance and Impact of the Study: The simultaneous study of the genomic methylation and proteome is important to correlate epigenetic modifications with gene expression and pathogen virulence.por
dc.description.sponsorshipFCTpor
dc.identifier.citationJ Appl Microbiol. 2013 Jun;114(6):1817-32. doi: 10.1111/jam.12187. Epub 2013 Apr 4por
dc.identifier.issn1364-5072
dc.identifier.issndoi: 10.1128/JCM.00302-13
dc.identifier.urihttp://hdl.handle.net/10400.18/1688
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWiley-Blackwellpor
dc.relationPPCDT/SAL-IMI/57297/2004por
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/jam.12187/abstract;jsessionid=2236FA47F8D01A4D273CAE5BD294A935.d01t02por
dc.subjectHelicobacter pyloripor
dc.subjectProteomicspor
dc.subjectGenomic Methylationpor
dc.subjectPhylogeneticspor
dc.subjectInfecções Gastrointestinaispor
dc.titleProteome variability among Helicobacter pylori isolates clustered according to genomic methylationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1832por
oaire.citation.startPage1817por
oaire.citation.titleJournal of Applied Microbiologypor
oaire.citation.volume114(6)por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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