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Proteome variability among Helicobacter pylori isolates clustered according to genomic methylation
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Orientador(es)
Resumo(s)
Aims: To understand whether the variability found in the proteome of
Helicobacter pylori relates to the genomic methylation, virulence and associated
gastric disease.
Methods and Results: We applied the Minimum-Common-Restriction-
Modification (MCRM) algorithm to genomic methylation data of 30
Portuguese H. pylori strains, obtained by genome sensitivity to Type II
restriction enzymes’ digestion. All the generated dendrograms presented three
clusters with no association with gastric disease. Comparative analysis of twodimensional
gel electrophoresis (2DE) maps obtained for total protein extracts
of 10 of these strains, representative of the three main clusters, revealed that
among 70 matched protein spots (in a universe of 300), 16 were differently
abundant (P < 0 05) among clusters. Of these, 13 proteins appear to be related
to the cagA genotype or gastric disease. The abundance of three protein
species, DnaK, GlnA and HylB, appeared to be dictated by the methylation
status of their gene promoter.
Conclusions: Variations in the proteome profile of strains with common
geographic origin appear to be related to differences in cagA genotype or
gastric disease, rather than to clusters organized according to strain genomic
methylation.
Significance and Impact of the Study: The simultaneous study of the genomic
methylation and proteome is important to correlate epigenetic modifications
with gene expression and pathogen virulence.
Descrição
Palavras-chave
Helicobacter pylori Proteomics Genomic Methylation Phylogenetics Infecções Gastrointestinais
Contexto Educativo
Citação
J Appl Microbiol. 2013 Jun;114(6):1817-32. doi: 10.1111/jam.12187. Epub 2013 Apr 4