Repository logo
 
Publication

Assessment of Beneficial and Possible Toxic Effects of Two New Alfalfa-Derived Shelf Products

2016-10Journal article Restricted access
Simple item page
dc.contributor.authorSoto-Zarazúa, María
dc.contributor.authorBah, Moustapha
dc.contributor.authorGarcía-Alcocer, María
dc.contributor.authorBerumen, Laura
dc.contributor.authorCosta, Carla Sofia
dc.contributor.authorBessa, Maria João
dc.contributor.authorRodrigues, Francisca
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorOliveira, Maria Beatriz
dc.date.accessioned2017-03-09T16:54:19Z
dc.date.available2017-03-09T16:54:19Z
dc.date.issued2016-10
dc.description.abstractAerial parts of Medicago sativa L. have been used as food and its consumption has been associated with health benefits, one among the most important being menopausal symptoms control. This work was aimed to explore possible pharmacological effects of two new alfalfa-derived products that have recently emerged as daily beverage preparations. In exploring their potential estrogenic effects, they produced no relevant alteration in the uterus. However, lowering glucose levels until normal values without causing further hypoglycemic effect were observed, when rats were treated with 1.5 g/kg/day samples. In vivo acute toxicity was not found when the alfalfa products were tested up to 3 g/kg rat weight. Furthermore, in vitro studies were conducted to assess their possible toxic effects. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase tests were carried out on the Caco-2 cell model to determine cell viability and membrane integrity. A concentration-dependent effect was observed, with a significant decrease in cell viability after exposure to concentrations of alfalfa product up to 100 mg/mL (after 3 h of incubation) and 50 mg/mL (after 24 h of treatment). Although in vitro level, the decrease in cell viability at these still low doses may underlie some toxicity, making necessary additional studies before any recommendation of a sustained consumption of these products by humans.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Med Food. 2016 Oct;19(10):970-7. doi:10.1089/jmf.2016.0024pt_PT
dc.identifier.doi10.1089/jmf.2016.0024pt_PT
dc.identifier.issn1096-620X
dc.identifier.urihttp://hdl.handle.net/10400.18/4590
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMary Ann Liebert/ Korean Society of Food Science and Nutritionpt_PT
dc.relation.publisherversionhttp://online.liebertpub.com/doi/abs/10.1089/jmf.2016.0024pt_PT
dc.subjectAlfalfapt_PT
dc.subjectCaco-2 Cellspt_PT
dc.subjectCell Viabilitypt_PT
dc.subjectEstrogenicpt_PT
dc.subjectHypoglycemicpt_PT
dc.subjectToxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleAssessment of Beneficial and Possible Toxic Effects of Two New Alfalfa-Derived Shelf Productspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage977pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage970pt_PT
oaire.citation.titleJournal of Medicinal Foodpt_PT
oaire.citation.volume19pt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Assessment of Beneficial.pdf
Size:
296.67 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DSA - Artigos em revistas internacionais