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Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillance

2021-08-25Journal article Open access
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dc.contributor.authorAhmed, Warish
dc.contributor.authorSimpson, Stuart
dc.contributor.authorBertsch, Paul
dc.contributor.authorBibby, Kyle
dc.contributor.authorBivins, Aaron
dc.contributor.authorBlackall, Linda
dc.contributor.authorBofill-Mas, Silvia
dc.contributor.authorBosch, Albert
dc.contributor.authorBrandao, Joao
dc.contributor.authorChoi, Phil
dc.contributor.authorCiesielski, Mark
dc.contributor.authorDonner, Erica
dc.contributor.authorD'Souza, Nishita
dc.contributor.authorFarnleitner, Andreas
dc.contributor.authorGerrity, Daniel
dc.contributor.authorGonzalez, Raul
dc.contributor.authorGriffith, John
dc.contributor.authorGyawali, Pradip
dc.contributor.authorHaas, Charles
dc.contributor.authorHamilton, Kerry
dc.contributor.authorHapuarachchi, Chanditha
dc.contributor.authorHarwood, Valerie
dc.contributor.authorHaque, Rehnuma
dc.contributor.authorJackson, Greg
dc.contributor.authorKhan, Stuart
dc.contributor.authorKhan, Wesaal
dc.contributor.authorKitajima, Masaaki
dc.contributor.authorKorajkic, Asja
dc.contributor.authorLa Rosa, Giuseppina
dc.contributor.authorLayton, Blythe
dc.contributor.authorLipp, Erin
dc.contributor.authorMcLellan, Sandra
dc.contributor.authorMcMinn, Brian
dc.contributor.authorMedema, Gertjan
dc.contributor.authorMetcalfe, Suzanne
dc.contributor.authorMeijer, Wim
dc.contributor.authorMueller, Jochen
dc.contributor.authorMurphy, Heather
dc.contributor.authorNaughton, Colleen
dc.contributor.authorNoble, Rachel
dc.contributor.authorPayyappat, Sudhi
dc.contributor.authorPetterson, Susan
dc.contributor.authorPitkanen, Tarja
dc.contributor.authorRajal, Veronica
dc.contributor.authorReyneke, Brandon
dc.contributor.authorRoman, Fernando
dc.contributor.authorRose, Joan
dc.contributor.authorRusinol, Marta
dc.contributor.authorSadowsky, Michael
dc.contributor.authorSala-Comorera, Laura
dc.contributor.authorSetoh, Yin Xiang
dc.contributor.authorSherchan, Samendra
dc.contributor.authorSirikanchana, Kwanrawee
dc.contributor.authorSmith, Wendy
dc.contributor.authorSteele, Joshua
dc.contributor.authorSabburg, Rosalie
dc.contributor.authorSymonds, Erin
dc.contributor.authorThai, Phong
dc.contributor.authorThomas, Kevin
dc.contributor.authorTynan, Josh
dc.contributor.authorToze, Simon
dc.contributor.authorThompson, Janelle
dc.contributor.authorWhiteley, Andy
dc.contributor.authorWong, Judith
dc.contributor.authorSano, Daisuke
dc.contributor.authorWuertz, Stefan
dc.contributor.authorXagoraraki, Irene
dc.contributor.authorZhang, Qian
dc.contributor.authorZimmer-Faust, Amity
dc.contributor.authorShanks, Orin
dc.date.accessioned2022-01-27T17:40:16Z
dc.date.available2022-01-27T17:40:16Z
dc.date.issued2021-08-25
dc.description.abstractWastewater surveillance for pathogens using the reverse transcription-polymerase chain reaction (RT-PCR) is an effective, resource-efficient tool for gathering additional community-level public health information, including the incidence and/or prevalence and trends of coronavirus disease-19 (COVID-19). Surveillance of SARS-CoV-2 in wastewater may provide an early-warning signal of COVID-19 infections in a community. The capacity of the world’s environmental microbiology and virology laboratories for SARS-CoV-2 RNA characterization in wastewater is rapidly increasing. However, there are no standardized protocols nor harmonized quality assurance and quality control (QA/QC) procedures for SARS-CoV-2 wastewater surveillance. This paper is a technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors. Recommendations include, stringent QA/QC measures, representative sampling approaches, effective virus concentration and efficient RNA extraction, amplification inhibition assessment, inclusion of sample processing controls, and considerations for RT-PCR assay selection and data interpretation. Clear data interpretation guidelines (e.g., determination of positive and negative samples) are critical, particularly during a low incidence of SARS-CoV-2 in wastewater. Corrective and confirmatory actions must be in place for inconclusive and/or potentially significant results (e.g., initial onset or reemergence of COVID-19 in a community). It will also be prudent to perform inter-laboratory comparisons to ensure results are reliable and interpretable for ongoing and retrospective analyses. The strategies that are recommended in this review aim to improve SARS-CoV-2 characterization for wastewater surveillance applications. A silver lining of the COVID-19 pandemic is that the efficacy of wastewater surveillance was demonstrated during this global crisis. In the future, wastewater will play an important role in the surveillance of a range of other communicable diseases.pt_PT
dc.description.abstractHighlights: Harmonized QA/QC procedures for SARS-CoV-2 wastewater surveillance are lacking; Wastewater analysis protocols are not optimized for trace analysis of viruses; False-positive and -negative errors have consequences for public health responses; Inter-laboratory studies utilizing standardized reference materials and protocols are needed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSci Total Environ. 2022 Jan 20;805:149877. doi: 10.1016/j.scitotenv.2021.149877. Epub 2021 Aug 25. Reviewpt_PT
dc.identifier.doi10.1016/j.scitotenv.2021.149877pt_PT
dc.identifier.issn1879-1026
dc.identifier.urihttp://hdl.handle.net/10400.18/7884
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0048969721049524?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCOVID-19pt_PT
dc.subjectSARS-CoV-2pt_PT
dc.subjectWastewaterpt_PT
dc.subjectSurveillancept_PT
dc.subjectFalse-positivept_PT
dc.subjectFalse-negativept_PT
dc.subjectRT-PCRpt_PT
dc.titleMinimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillancept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage149877pt_PT
oaire.citation.titleScience of the Total Environmentpt_PT
oaire.citation.volume805pt_PT
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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