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  • Methylmercury: A Human Biomonitoring Study of Portuguese Women of Childbearing Age
    Publication . Santiago, Susana; Namorado, Sónia; Matias Dias, Carlos; Martins, Carla; Carvalho, Cristina; Assunção, Ricardo
    BACKGROUND AND AIM: Methylmercury (MeHg) is a toxic compound that undergoes bioaccumulation in the aquatic food chain. Predatory fish species are an important human exposure source. Portugal has the highest consumption of fishery products in the European Union (EU), above the EU and world average. The main target of MeHg is the central nervous system and the prenatal period represents a period of greatest vulnerability regarding neurodevelopmental effects on the foetus. Human biomonitoring (HBM) allows direct exposure assessment of human exposure to chemical compounds. Total mercury (THg) concentration in blood is usually considered an appropriate biomarker for estimating short-term internal exposure to MeHg in individuals with regular fish consumption. The aim of the present study was to evaluate the exposure of Portuguese women of childbearing age to MeHg through HBM. METHODS: Three hundred Portuguese women of childbearing age (25 to 44 years) were randomly selected among the participants in a cross-sectional epidemiological study carried out in Portugal (INSEF) in 2015. THg content was determined in whole blood samples by thermal decomposition and amalgamation atomic absorption spectrophotometry (TDA/AAS). RESULTS:Two samples had THg levels below LOQ (0.5 µg/L), and in the remaining (n=298), THg levels ranged from 0.6 to 35.0 µg/L. About 52% of the samples had values below 5 µg/L, a HBM value below which no adverse health effects are expected. However, 48% of samples revealed levels above 5 µg/L and therefore presented risk of adverse health effects. Blood mercury concentration values were higher in older women, in higher education level and residents in the Autonomous Region of Madeira, with statistically significant differences compared to the other groups (p<0.05). CONCLUSIONS:This study reinforces the need to develop and implement in Portugal strategies regarding risk communication focused on the selection of fish species with lower MeHg concentration in order to reduce human exposure to this compound.
    2021-08-25Conference object Restricted access Show more
  • Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillance
    Publication . Ahmed, Warish; Simpson, Stuart; Bertsch, Paul; Bibby, Kyle; Bivins, Aaron; Blackall, Linda; Bofill-Mas, Silvia; Bosch, Albert; Brandao, Joao; Choi, Phil; Ciesielski, Mark; Donner, Erica; D'Souza, Nishita; Farnleitner, Andreas; Gerrity, Daniel; Gonzalez, Raul; Griffith, John; Gyawali, Pradip; Haas, Charles; Hamilton, Kerry; Hapuarachchi, Chanditha; Harwood, Valerie; Haque, Rehnuma; Jackson, Greg; Khan, Stuart; Khan, Wesaal; Kitajima, Masaaki; Korajkic, Asja; La Rosa, Giuseppina; Layton, Blythe; Lipp, Erin; McLellan, Sandra; McMinn, Brian; Medema, Gertjan; Metcalfe, Suzanne; Meijer, Wim; Mueller, Jochen; Murphy, Heather; Naughton, Colleen; Noble, Rachel; Payyappat, Sudhi; Petterson, Susan; Pitkanen, Tarja; Rajal, Veronica; Reyneke, Brandon; Roman, Fernando; Rose, Joan; Rusinol, Marta; Sadowsky, Michael; Sala-Comorera, Laura; Setoh, Yin Xiang; Sherchan, Samendra; Sirikanchana, Kwanrawee; Smith, Wendy; Steele, Joshua; Sabburg, Rosalie; Symonds, Erin; Thai, Phong; Thomas, Kevin; Tynan, Josh; Toze, Simon; Thompson, Janelle; Whiteley, Andy; Wong, Judith; Sano, Daisuke; Wuertz, Stefan; Xagoraraki, Irene; Zhang, Qian; Zimmer-Faust, Amity; Shanks, Orin
    Wastewater surveillance for pathogens using the reverse transcription-polymerase chain reaction (RT-PCR) is an effective, resource-efficient tool for gathering additional community-level public health information, including the incidence and/or prevalence and trends of coronavirus disease-19 (COVID-19). Surveillance of SARS-CoV-2 in wastewater may provide an early-warning signal of COVID-19 infections in a community. The capacity of the world’s environmental microbiology and virology laboratories for SARS-CoV-2 RNA characterization in wastewater is rapidly increasing. However, there are no standardized protocols nor harmonized quality assurance and quality control (QA/QC) procedures for SARS-CoV-2 wastewater surveillance. This paper is a technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors. Recommendations include, stringent QA/QC measures, representative sampling approaches, effective virus concentration and efficient RNA extraction, amplification inhibition assessment, inclusion of sample processing controls, and considerations for RT-PCR assay selection and data interpretation. Clear data interpretation guidelines (e.g., determination of positive and negative samples) are critical, particularly during a low incidence of SARS-CoV-2 in wastewater. Corrective and confirmatory actions must be in place for inconclusive and/or potentially significant results (e.g., initial onset or reemergence of COVID-19 in a community). It will also be prudent to perform inter-laboratory comparisons to ensure results are reliable and interpretable for ongoing and retrospective analyses. The strategies that are recommended in this review aim to improve SARS-CoV-2 characterization for wastewater surveillance applications. A silver lining of the COVID-19 pandemic is that the efficacy of wastewater surveillance was demonstrated during this global crisis. In the future, wastewater will play an important role in the surveillance of a range of other communicable diseases.
    2021-08-25Journal article Open access Show more
  • The value of Human Biomonitoring to assess chemical exposure and support policies: perceptions of the European population
    Publication . Namorado, Sónia; Katsonour, Andromachi; Reynders, Hans; Mampaey, Maja; Tarroja, Elena; Barouki, Robert; Louro, Henriqueta; Isidro, Glória; Silva, Maria João; Bourqui, Martine; Von Goetz, Natalie; Sepai, Ovnair; Lobo Vicente, Joana
    BACKGROUND AND AIM:The development of Human Biomonitoring (HBM) surveys is highly dependent on the cooperation and engagement of the individuals being sampled. As such, in the context of the European Human Biomonitoring Initiative (HBM4EU) an online citizen survey was conducted in Europe to gain insights into the perceptions of the population about HBM and exposure to chemicals. METHODS:The survey was undertaken between September 2020 and February 2021 and was available in the languages of all 30 HBM4EU-participating countries (EU, associated countries, Israel) through the HBM4EU website. Dissemination was done by the HBM4EU country representatives (National Hub Contact Points). RESULTS:Responses were received from 5391 participants. Around half were 35-54 years old and the majority were female, with higher education and employed. Concerning the perception of the respondents on HBM, the majority considers it should be done (81.7%), even if the chemicals are considered well managed. Most participants consider it a reliable method (84.0%) that should be performed more often (87.3%) and be more coordinated either at a European (86.2%) or at a national level (83.7%). The large majority of the respondents thinks that HBM results are important to evaluate the chemical exposure of the population (96.2%), to study the health impact of chemical exposure (96.3%) and to support the development of health policies (94.9%). The respondents also consider HBM results highly relevant to raise awareness and promote understanding of the impact of chemical exposure amongst the general population (94.3%), health professionals and policy makers. CONCLUSIONS:The use of HBM as a tool to assess human exposure to chemicals, allowing its management and health protection, was well perceived by respondents across Europe. Furthermore, the need for more studies conducted in a coordinated way was indicated. Future work should comprise population groups less represented here and should also attempt to understand how perceptions change over time.
    2021-08-25Conference object Open access Show more