In vitro acute toxicity of metal-based nanoparticles in human trophoblast BeWo b30 cells

Pires, J.; Moreira, L.; Teixeira, J.P.; Fraga, S.

http://hdl.handle.net/10400.18/8317

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
1-s2.0-S0378427421005051-main.pdf		68.66 KB	Adobe PDF	Download

Send Feedback

Authors

Abstract(s)

Metal nanoparticles (M-NP) are among the most widely used nanomaterials in consumer products available in the market. Thus, human exposure to these nanosized materials is increasing, which raises serious concerns regarding their environmental and human safety. Biological barriers are important lines of defence to xenobiotics, thus expected targets for M-NP. In this regard, special consideration must be given to the placenta that acts as barrier between maternal and the developing fetus. The present study aimed at evaluating in vitro toxicity of different M-NP in a human cell model of placental barrier: trophoblastic (BeWo clone b30) epithelial cells. BeWo b30 cells were exposed for 24 h to varied concentrations (0.8–48 µg/cm2) of M-NP of different chemical composition (Au, Ag and TiO2), primary size (10, 30 and 60 nm), capping (citrate and PEG) and crystal structure (rutile and anatase).