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Estudo para a determinação dos valores de referência das enzimas aspartato aminnotransferase (AST), alanina aminotransferase (ALT) e gama- glutamil transferase (GGT), no soro
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Advisor(s)
Abstract(s)
É recomendável que cada laboratório se baseie em protocolos e procedimentos operacionais comuns com a finalidade de obter valores de referência “comuns” e “harmonizados” a nível nacional. O objetivo deste trabalho foi determinar os valores de referência das enzimas AST, ALT e
GGT. A metodologia teve como base o documento CSLI / IFCC, C28-A3.
A amostra inicial foi de 1688 indivíduos de Portugal continental, dos quais
1122 foram excluídos após a análise dos questionários (com patologias
e/ou fumadores) de modo a obtermos uma população considerada “saudável”. Após a quantificação das enzimas por um método enzimático, verificou-se que não houve relevância nos resultados para diferentes grupos
etários, embora tenha sido observada diferença estatística significativa
quanto ao género ( p<0,001). Desta forma, foram calculados os valores
de referência para o género feminino e masculino respetivamente da
ALT = 7-38U/L e 10-70U/L, da AST = 11-30U/L e 13-53U/L e da GGT =
5-36U/L e 7-80U/L, através do programa IBM SPSS Statistics versão 25.
Os valores de referência, no caso do género feminino, foram muito semelhantes aos descritos nos folhetos informativos, enquanto no género masculino verificaram-se valores superiores.
It is recommended that each laboratory base itself on common protocols and operating procedures in order to achieve “standard” reference values at national level. The aim of this work is to determine the reference values of the enzymes AST, ALT and GGT. The methodology used was based on the document CSLI / IFCC, C28-A3. From the initial sample of 1688 individuals from mainland Portugal, 1122 were excluded due to the analysis of the questionnaires, (whith pathologies and/or smokers) to obtein a population considered healthy We studied 566 serum samples and quantification of enzymes by an enzymatic method in Cobas INTEGRA 400 Plus, showed no significance diferences in the results for several age groups, although a statistically significant difference was observed regarding gender (p<0,001). Therefore, the reference values for females and males respectively were calculated for ALT = 7-38U/L and 10-70U/L, for AST = 11-30U/L and 13-53U/L and for GGT = 5- 36U/L and 7-80U/L, through the IBM SPSS Statistics version 25 program. The reference values, for females, were very similar to those described by other publications, while for males, higher values were found.
It is recommended that each laboratory base itself on common protocols and operating procedures in order to achieve “standard” reference values at national level. The aim of this work is to determine the reference values of the enzymes AST, ALT and GGT. The methodology used was based on the document CSLI / IFCC, C28-A3. From the initial sample of 1688 individuals from mainland Portugal, 1122 were excluded due to the analysis of the questionnaires, (whith pathologies and/or smokers) to obtein a population considered healthy We studied 566 serum samples and quantification of enzymes by an enzymatic method in Cobas INTEGRA 400 Plus, showed no significance diferences in the results for several age groups, although a statistically significant difference was observed regarding gender (p<0,001). Therefore, the reference values for females and males respectively were calculated for ALT = 7-38U/L and 10-70U/L, for AST = 11-30U/L and 13-53U/L and for GGT = 5- 36U/L and 7-80U/L, through the IBM SPSS Statistics version 25 program. The reference values, for females, were very similar to those described by other publications, while for males, higher values were found.
Description
Keywords
Aspartato Aminnotransferase Alanina Aminotransferase Gama- glutamil Transferase Enzimas Valores de Referência Análises laboratoriais Doenças Hepatobiliares Portugal
Pedagogical Context
Citation
Boletim Epidemiológico Observações. 2022 janeiro-abril;11(31):44-48
Publisher
Instituto Nacional de Saúde Doutor Ricardo Jorge, IP