Publicação
Antioxidant enzymes activities in Mytilus galloprovincialis exposed to 17α -ethinylestradiol (ee2): potential implications for human health
| dc.contributor.author | Copeto, Sandra | |
| dc.contributor.author | Ferreira, Inês João | |
| dc.contributor.author | Motta, Carla | |
| dc.contributor.author | Silva, Marco | |
| dc.contributor.author | Diniz, Mário | |
| dc.date.accessioned | 2026-03-04T15:47:37Z | |
| dc.date.available | 2026-03-04T15:47:37Z | |
| dc.date.issued | 2025-05-08 | |
| dc.description.abstract | Introduction: The impact of endocrine-disrupting compounds (EDCs) on marine biota has been extensively studied, with particular focus on the effects of exposure to 17α-ethinylestradiol (EE2), the active ingredient in female contraceptives, which is commonly found in urban and industrial effluents and consequently discharged into the aquatic environment, raising concerns about its ecological effects. Aims: This study aimed to investigate the adverse effects of EE2 using the mussel (Mytilus galloprovincialis) as a biological model. Methodology: Mussels with an average length of 2.9 ± 0.3 cm, were exposed to environmentally relevant concentrations of EE2 (0, 10, 100, and 1000 ng/L) for 28 days. Various biomarkers, including antioxidant enzymes, lipid peroxidation, and total ubiquitin (UBI), were analysed to assess the effects of EE2 exposure. Results: Glutathione S-transferase (GST) and catalase (CAT) activities showed statistically significant differences, with GST differing between controls and the lowest (10 ng/L) and highest (1000 ng/L) concentrations. CAT activity varied significantly between controls and the 10 and 100 ng/L groups and between the 10 and 1000 ng/L and 100 and 1000 ng/L groups. In contrast, malondialdehyde (MDA) and superoxide dismutase (SOD) levels remained unchanged across treatments. UBI levels increased in mussels exposed to the highest concentration, but no significant differences were observed between controls and EE2 concentrations tested. Conclusion: This study highlights the potential ecotoxicological impacts of EE2 in marine environments, particularly its effects on oxidative stress and protein damage in M. galloprovincialis. The findings are also relevant to human health, as EE2 contamination in aquatic ecosystems can lead to bioaccumulation in seafood, increasing the risk of endocrine disruption and oxidative stress-related disorders. The results suggest an increase in antioxidant enzyme activity, while markers of oxidative damage remained unchanged. Prolonged exposure to EE2 through contaminated water or food may contribute to hormonal imbalances, reproductive dysfunction, and heightened susceptibility to chronic diseases. | eng |
| dc.description.sponsorship | The authors would like to thank funding from FCT under the strategic project UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/11143 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.relation | Institute for Health and Bioeconomy | |
| dc.relation | Applied Molecular Biosciences Unit | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Mytilus Galloprovincialis | |
| dc.subject | 17α-Ethinylestradiol (EE2) | |
| dc.subject | Environmental Contaminants | |
| dc.subject | Mussels | |
| dc.subject | Segurança Alimentar | |
| dc.title | Antioxidant enzymes activities in Mytilus galloprovincialis exposed to 17α -ethinylestradiol (ee2): potential implications for human health | eng |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.awardNumber | LA/P/0140/2020 | |
| oaire.awardNumber | UIDB/04378/2020 | |
| oaire.awardTitle | Institute for Health and Bioeconomy | |
| oaire.awardTitle | Applied Molecular Biosciences Unit | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT | |
| oaire.citation.conferenceDate | 2025-05-08 | |
| oaire.citation.conferencePlace | Braga, Portugal | |
| oaire.citation.title | XXIV Congresso de Nutrição e Alimentação – O Tempo da Nutrição, 8, 9 maio 2025 | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| relation.isProjectOfPublication | ea242207-577b-433b-a1f6-8fc1a6385add | |
| relation.isProjectOfPublication | b6e46a61-d241-4879-b841-169d21cd75b9 | |
| relation.isProjectOfPublication.latestForDiscovery | ea242207-577b-433b-a1f6-8fc1a6385add |