Effect of cytochalasin-B on TIO2 nanoparticles uptake by different cell lines

Brandão, Fátima; Costa, Carla; Fraga, Sónia; Valdiglesias, Vanessa; Fernández-Bertólez, Natalia; Laffon, Blanca; Pásaro, Eduardo; Teixeira, João Paulo

http://hdl.handle.net/10400.18/6089

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Authors

Brandão, Fátima

Costa, Carla

Fraga, Sónia

Valdiglesias, Vanessa

Fernández-Bertólez, Natalia

Laffon, Blanca

Pásaro, Eduardo

Teixeira, João Paulo

Abstract(s)

The in vitro cytokinesis-block micronucleus cytome assay (CBMN) is widely used for genotoxicity evaluation of nanomaterials (NMs). However, cytochalasin-B (Cyt-B) used in this test may affect the uptake of NMs due to possible interference with endocytosis. Thus, the aim of this study was to investigate the influence of Cyt-B on the uptake of TiO2 nanoparticles (TiO2 NPs) by four different human cell lines: lung cells (A549), glial cells (A172), neurons (SH-SY5Y), and liver cells (HepG2).