Functional effects of differentially expressed microRNAs in alveolar epithelial cells exposed to MWCNT-7 or crocidolite

Ventura, Célia; Vieira, Luís; Silva, Catarina; Sousa-Uva, Antonio; Silva, Maria João

http://hdl.handle.net/10400.18/7054

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Background and Aims Multi-walled carbon nanotubes (MWCNT) are widely used engineered nanomaterials in industrial and biomedical applications, but their inhalation may induce pulmonary adverse effects. Moreover, the MWCNT-7 (Mitsui-7) has been classified as possibly carcinogenic to humans. Nevertheless, MWCNT-7 molecular modes of action are poorly understood and there are no biomarkers of exposure or effect for occupational monitoring. Recently, several pulmonary diseases, including lung cancer, have been associated with alterations in microRNA expression. These changes are potentially useful to detect or evaluate disease progression and to understand the molecular effects induced by the disease or toxicant. In this study, we describe a set of differentially expressed microRNAs (DE miRNAs) in A549 epithelial alveolar cells, following exposure to an occupationally relevant dose of MWCNT-7 or crocidolite. The functional pathways and ontology terms enriched with genes targeted by DE miRNAs were identified. Moreover, an interaction network of DE miRNAs-target cancer genes was constructed.