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Monitoring and molecular profiling of contemporary insecticide resistance status of malaria vectors in Guinea-Bissau

2020-06Journal article Restricted access
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dc.contributor.authorSilva, R.
dc.contributor.authorMavridis, K.
dc.contributor.authorVontas, J.
dc.contributor.authorRodrigues, A.
dc.contributor.authorOsório, H.
dc.date.accessioned2021-03-04T15:36:34Z
dc.date.available2021-03-04T15:36:34Z
dc.date.issued2020-06
dc.descriptionDocumento em depósito não pode ser disponibilizado publicamente.pt_PT
dc.description.abstractDespite reduction in the prevalence of malaria, Guinea-Bissau (GB) is still widely affected by the disease that is primarily vectored by Anopheles gambiae s.l. mosquitoes. Monitoring mosquito susceptibility and investigating the insecticide resistance status is an integral part of malaria control actions. Here, mosquito populations from five regions of GB: Bafatá, Bissau, Buba, Cacheu and Gabu were monitored for species ID and insecticide resistance, using diagnostic and intensity WHO bioassays, as well as molecular assays. Phenotypic and molecular identification of species showed the presence of An. gambiae s.s. (S form), An. coluzzii (M form) and An. arabiensis, as well as rare An. arabiensis/ An. gambiae hybrids. Resistance to permethrin and deltamethrin was found in all Anopheles populations assayed, with the intensity of resistance for permethrin being moderate to high, as confirmed by bioassays performed at concentration intensities of 5X and 10X. Consistent to these findings, molecular analysis showed a higher frequency of knock-down resistance (kdr) mutations (L1014F, L1014S, reaching > 90% in some areas) compared to previous studies in the same region, as well as detected for the first time the presence of the super kdr mutation (N1575Y) in GB. The “iAche” (G119S) resistance mutation was also found in GB in low frequencies (up to 12.41%). Additionally, the synergistic PBO-permethrin bioassays suggested partial involvement of non target (metabolic and/or reduced penetration) resistance mechanism. Expression analysis of known pyrethroid metabolisers indicated the slight overexpression and possible association of the cytochrome P450s CYP6Z1, CYP4G16 with the pyrethroid resistance phenotype. The findings should guide future evidence-based resistance management strategies in GB.pt_PT
dc.description.abstractHighlights: High insecticide resistance level in anopheles from Guinea-Bissau; High frequency of kdr and first time detection of the super kdr mutation (N1575Y); First detection of the iAche (G119S) mutation; Detection of An. gambiae/arabiensis hybrids; First study to analyze detoxification gene expression in Guinea-Bissau.pt_PT
dc.description.sponsorshiphe insecticide susceptibility tests study was funded by The Global Funds to Fight AIDS, Tuberculosis and Malaria through UNDP GuineaBissau. Molecular genotyping was sponsored by the European Union’s Horizon 2020 research and innovation program INFRAVEC2 under grant agreement No 731060.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationActa Trop. 2020 Jun;206:105440. doi: 10.1016/j.actatropica.2020.105440. Epub 2020 Mar 8.pt_PT
dc.identifier.doi10.1016/j.actatropica.2020.105440pt_PT
dc.identifier.issn0001-706X
dc.identifier.urihttp://hdl.handle.net/10400.18/7315
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationResearch Infrastructures for the control of vector-borne diseases
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/acta-tropicapt_PT
dc.subjectMosquitoespt_PT
dc.subjectAnopheles gambiaept_PT
dc.subjectMalariapt_PT
dc.subjectInsecticide resistancept_PT
dc.subjectKdrpt_PT
dc.subjectIachept_PT
dc.subjectAce-1pt_PT
dc.subjectDetoxification enzymespt_PT
dc.subjectP450spt_PT
dc.subjectGuinea-Bissaupt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleMonitoring and molecular profiling of contemporary insecticide resistance status of malaria vectors in Guinea-Bissaupt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleResearch Infrastructures for the control of vector-borne diseases
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/731060/EU
oaire.citation.startPage105440pt_PT
oaire.citation.titleActa Tropicapt_PT
oaire.citation.volume206pt_PT
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationf3035277-fb14-4956-ab59-b6a9d871d600
relation.isProjectOfPublication.latestForDiscoveryf3035277-fb14-4956-ab59-b6a9d871d600

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