Monitoring and molecular profiling of contemporary insecticide resistance status of malaria vectors in Guinea-Bissau

Silva, R.; Mavridis, K.; Vontas, J.; Rodrigues, A.; Osório, H.

http://hdl.handle.net/10400.18/7315

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Abstract(s)

Despite reduction in the prevalence of malaria, Guinea-Bissau (GB) is still widely affected by the disease that is primarily vectored by Anopheles gambiae s.l. mosquitoes. Monitoring mosquito susceptibility and investigating the insecticide resistance status is an integral part of malaria control actions. Here, mosquito populations from five regions of GB: Bafatá, Bissau, Buba, Cacheu and Gabu were monitored for species ID and insecticide resistance, using diagnostic and intensity WHO bioassays, as well as molecular assays. Phenotypic and molecular identification of species showed the presence of An. gambiae s.s. (S form), An. coluzzii (M form) and An. arabiensis, as well as rare An. arabiensis/ An. gambiae hybrids. Resistance to permethrin and deltamethrin was found in all Anopheles populations assayed, with the intensity of resistance for permethrin being moderate to high, as confirmed by bioassays performed at concentration intensities of 5X and 10X. Consistent to these findings, molecular analysis showed a higher frequency of knock-down resistance (kdr) mutations (L1014F, L1014S, reaching > 90% in some areas) compared to previous studies in the same region, as well as detected for the first time the presence of the super kdr mutation (N1575Y) in GB. The “iAche” (G119S) resistance mutation was also found in GB in low frequencies (up to 12.41%). Additionally, the synergistic PBO-permethrin bioassays suggested partial involvement of non target (metabolic and/or reduced penetration) resistance mechanism. Expression analysis of known pyrethroid metabolisers indicated the slight overexpression and possible association of the cytochrome P450s CYP6Z1, CYP4G16 with the pyrethroid resistance phenotype. The findings should guide future evidence-based resistance management strategies in GB.

Highlights: High insecticide resistance level in anopheles from Guinea-Bissau; High frequency of kdr and first time detection of the super kdr mutation (N1575Y); First detection of the iAche (G119S) mutation; Detection of An. gambiae/arabiensis hybrids; First study to analyze detoxification gene expression in Guinea-Bissau.

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Keywords

Mosquitoes Anopheles gambiae Malaria Insecticide resistance Kdr Iache Ace-1 Detoxification enzymes P450s Guinea-Bissau Infecções Sistémicas e Zoonoses