Publication
Diagnostic Assays for Crimean-Congo hemorrhagic fever
| dc.contributor.author | Vanhomwegen, J. | |
| dc.contributor.author | Alves, M.J. | |
| dc.contributor.author | Zupanc, T.A. | |
| dc.contributor.author | Bino, S. | |
| dc.contributor.author | Chinikar, S. | |
| dc.contributor.author | Karlberg, H. | |
| dc.contributor.author | Korukluoglu, G. | |
| dc.contributor.author | Korva, M. | |
| dc.contributor.author | Mardani, M. | |
| dc.contributor.author | Mirazimi, A. | |
| dc.contributor.author | Mousavi, M. | |
| dc.contributor.author | Papa, A. | |
| dc.contributor.author | Saksida, A. | |
| dc.contributor.author | Sharifi-Mood, B. | |
| dc.contributor.author | Sidira, P. | |
| dc.contributor.author | Tsergouli, K. | |
| dc.contributor.author | Wolfel, R. | |
| dc.contributor.author | Zeller, H. | |
| dc.contributor.author | Dubois, P. | |
| dc.date.accessioned | 2013-01-28T13:49:48Z | |
| dc.date.available | 2013-01-28T13:49:48Z | |
| dc.date.issued | 2012-12 | |
| dc.description.abstract | Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material. | por |
| dc.identifier.citation | Emerg Infect Dis. 2012 Dec;18(12):1958-65 | por |
| dc.identifier.issn | 1080-6040 | |
| dc.identifier.other | doi: 10.3201/eid1812.120710 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/1176 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | CDC-National Center for Infectious Diseases | por |
| dc.relation.publisherversion | http://wwwnc.cdc.gov/eid/article/18/12/12-0710_article.htm | por |
| dc.subject | Infecções Sistémicas e Zoonoses | por |
| dc.subject | Crimean-Congo | por |
| dc.subject | Hemorrhagic Fever | por |
| dc.subject | Diagnostic Assays | por |
| dc.title | Diagnostic Assays for Crimean-Congo hemorrhagic fever | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1965 | por |
| oaire.citation.startPage | 1958 | por |
| oaire.citation.title | Emerging Infectious Diseases | por |
| oaire.citation.volume | 18 | por |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
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