Diagnostic Assays for Crimean-Congo hemorrhagic fever

Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material.

Palavras-chave

Infecções Sistémicas e Zoonoses Crimean-Congo Hemorrhagic Fever Diagnostic Assays

URI

http://hdl.handle.net/10400.18/1176

Citação

Emerg Infect Dis. 2012 Dec;18(12):1958-65

Editora

CDC-National Center for Infectious Diseases

Coleções

DDI - Artigos em revistas internacionais

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