Publication
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
| dc.contributor.author | David, Susana | |
| dc.contributor.author | Sutre, Ana Filipa | |
| dc.contributor.author | Sanca, Armando | |
| dc.contributor.author | Mané, Alfredo | |
| dc.contributor.author | Henriques, Victor | |
| dc.contributor.author | Portugal, Clara | |
| dc.contributor.author | Sancho, Luísa | |
| dc.contributor.author | Cardoso, Ana | |
| dc.contributor.author | Paixão, Eleonora | |
| dc.contributor.author | Duarte, Elsa Leclerc | |
| dc.contributor.author | Leite, Clarice Queico Fujimura | |
| dc.contributor.author | Salem, Julia Ignez | |
| dc.contributor.author | Antunes, Abílio | |
| dc.date.accessioned | 2013-03-22T11:20:39Z | |
| dc.date.available | 2013-03-22T11:20:39Z | |
| dc.date.issued | 2012-11-01 | |
| dc.description.abstract | The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings. | por |
| dc.description.sponsorship | Luso-American Development Foundation (LACR Award program) | por |
| dc.identifier.citation | Int J Tuberc Lung Dis. 2012 Nov;16(11):1535-7. doi: 10.5588/ijtld.11.0658 | por |
| dc.identifier.issn | 1027-3719 | |
| dc.identifier.other | doi: 10.5588/ijtld.11.0658 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/1532 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | International Union Against Tuberculosis and Lung Disease | por |
| dc.relation.publisherversion | http://www.ingentaconnect.com/content/iuatld/ijtld/2012/00000016/00000011/art00020 | por |
| dc.subject | Mycobacterium Tuberculosis | por |
| dc.subject | rpoB | por |
| dc.subject | Sputum Smear Microscopy | por |
| dc.subject | Sodium Hypochlorite | por |
| dc.subject | Denaturing High-performance liquid chromatography (DHPLC) Sequencing | por |
| dc.subject | Infecções Respiratórias | por |
| dc.subject | Resistência aos Antimicrobianos | por |
| dc.title | Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1537 | por |
| oaire.citation.startPage | 1535 | por |
| oaire.citation.title | International Journal of Tuberculosis and Lung Disease | por |
| oaire.citation.volume | 16(11) | por |
| rcaap.rights | embargoedAccess | por |
| rcaap.type | article | por |