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Publicação
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
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Orientador(es)
Resumo(s)
The diagnosis of tuberculosis is seriously hampered in
the absence of standard biosafety laboratory facilities
for specimen concentration and Mycobacterium tuberculosis
culture. Within a laboratory twinning arrangement,
heat-fixed direct smear and sediment from
74 bleach-processed and 20 non-processed specimens
from Cumura Hospital, Guinea-Bissau, were sent to Lisbon
for molecular evaluation of rifampicin resistance.
Sequence analysis of a 369 base-pair rpoB locus detected
3.2% (3/94) resistant specimens. To our knowledge,
this represents the first report on the molecular analysis
of M. tuberculosis from bleach-processed sputum, an alternative
to current diagnostic practice in low-resource
settings.
Descrição
Palavras-chave
Mycobacterium Tuberculosis rpoB Sputum Smear Microscopy Sodium Hypochlorite Denaturing High-performance liquid chromatography (DHPLC) Sequencing Infecções Respiratórias Resistência aos Antimicrobianos
Contexto Educativo
Citação
Int J Tuberc Lung Dis. 2012 Nov;16(11):1535-7. doi: 10.5588/ijtld.11.0658
Editora
International Union Against Tuberculosis and Lung Disease