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- Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity buildingPublication . David, Susana; Sutre, Ana Filipa; Sanca, Armando; Mané, Alfredo; Henriques, Victor; Portugal, Clara; Sancho, Luísa; Cardoso, Ana; Paixão, Eleonora; Duarte, Elsa Leclerc; Leite, Clarice Queico Fujimura; Salem, Julia Ignez; Antunes, AbílioThe diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.