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Publication
Aumento de casos de Salmonella Typhimurium em 2024: caracterização fenotípica e genotípica dos isolados
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Advisor(s)
Abstract(s)
Salmonelose é a segunda zoonose mais frequente a nível mundial. É causada
por Salmonella enterica, sendo S. Typhimurium um dos serotipos mais
reportados na União Europeia. Um dos maiores riscos para a saúde pública
é a presença de Salmonella spp. em alimentos, e consequente associação
a surtos que podem atingir uma dimensão transfronteiriça.
Este estudo teve como principal objetivo caraterizar fenotípica e genotipicamente
isolados de Salmonella Typhimurium recebidos no Laboratório Nacional
de Referência de Infeções Gastrintestinais do Instituto Nacional de Saúde
Doutor Ricardo Jorge, na sequência do aumento considerável de isolados
deste serotipo observado entre abril e maio de 2024.
Foram estudados 83 isolados, dos quais 64 integravam o mesmo cluster
genético (77,1%), Cluster 1, identificado por core genome multilocus
sequence typing (Enterobase cgMLST V2 + HierCC V1). Todos os isolados
pertenciam à sequência tipo 19. Foi identificada a presença dos genes de
resistência blaCARB-2, sul1, aadA2, aac(6’)-Iaa, tet(G) e floR em 67 isolados,
perfil concordante com o fenótipo encontrado, AMP-AMC-TET-CHL. A
análise de cgMLST permitiu ainda identificar quatro isolados europeus que
integravam o Cluster 1.
Salmonellosis is the second most frequent zoonosis worldwide. It is caused by Salmonella enterica, with S. Typhimurium being one of the most repor ted serotypes in the European Union. One of the major public health risks is the presence of Salmonella spp. in food, leading to outbreaks that can reach cross-border dimensions. The main goal of this study was to characterise, phenotypically and genotypically, the Salmonella Typhimurium isolates received at the National Reference Laboratory for Gastrointestinal Infections of the National Institute of Health Doutor Ricardo Jorge, due to the considerable increase observed between April and May 2024. In this work, 83 isolates were studied, of which 64 (77.1%) belonged to the same cluster, Cluster 1, identified by core genome multilocus sequence typing (Enterobase cgMLST V2 + HierCC V1). All isolates belonged to sequence type 19. The resistance genes blaCARB-2, sul1, aadA2, aac(6’)-Iaa, tet(G) and floR were identified in 67 isolates, concordant with the phenotype detected, AMP-AMC-TET-CHL. Subsequent cgMLST analysis identified four isolates from other European countries that were also included in Cluster 1.
Salmonellosis is the second most frequent zoonosis worldwide. It is caused by Salmonella enterica, with S. Typhimurium being one of the most repor ted serotypes in the European Union. One of the major public health risks is the presence of Salmonella spp. in food, leading to outbreaks that can reach cross-border dimensions. The main goal of this study was to characterise, phenotypically and genotypically, the Salmonella Typhimurium isolates received at the National Reference Laboratory for Gastrointestinal Infections of the National Institute of Health Doutor Ricardo Jorge, due to the considerable increase observed between April and May 2024. In this work, 83 isolates were studied, of which 64 (77.1%) belonged to the same cluster, Cluster 1, identified by core genome multilocus sequence typing (Enterobase cgMLST V2 + HierCC V1). All isolates belonged to sequence type 19. The resistance genes blaCARB-2, sul1, aadA2, aac(6’)-Iaa, tet(G) and floR were identified in 67 isolates, concordant with the phenotype detected, AMP-AMC-TET-CHL. Subsequent cgMLST analysis identified four isolates from other European countries that were also included in Cluster 1.
Description
Keywords
Salmonella Typhimurium Caracterização Fenotípica Caracterização Genotípica Salmonelose Doenças Infeciosas Saúde Pública Portugal
Pedagogical Context
Citation
Boletim Epidemiológico Observações. 2023 mai-ago;12(34):16-20
Publisher
Instituto Nacional de Saúde Doutor Ricardo Jorge, IP