Establishing nanomaterial grouping/ classification strategies according to toxicity and biological effects for supporting risk assessment

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Publications

Comet assay assessment of oleic acid-coated magnetite nanoparticles on human SHSY5Y neuronal cells

Publication . Joao, Teixeira; Blanca, Laffon; Gözde, Kiliç; Natalia, Fernández-Bertólez; Carla, Costa; Solange, Costa; Eduardo, Passaro; Vanessa, Valdiglesias

Superparamagnetic iron oxide nanoparticles (ION) have a wide range of potential applications. Among them, the most important uses in biology and medicine are as contrast agents in magnetic resonance imaging, as carriers for drug delivery or transfection and as therapeutic agents in cancer therapy by magnetic field-mediated hyperthermia. For all these applications, ION must be introduced in the human body and come into contact with cells and tissues, so it is imperative to know the potential risks associated to this exposure. Nevertheless, although ION biocompatibility has been reported to be high, there is a lack of information regarding their genotoxic potential, especially on the nervous system. Thus, the main objective of this work was to examine possible genotoxic effects of ION (crystalline phase magnetite, covered by oleic acid) on human SHSY5Y neuronal cells by the standard alkaline comet assay, along with its OGG1 enzyme modified version to analyse oxidative DNA damage. Previously we evaluated the possible interference of the ION with the comet assay methodology and with OGG1 enzyme activity. ION were dispersed both in complete and serum-free cell culture media, and cells were exposed to four concentrations in the range 10-200 µg/ml for 3 and 24 h. Results obtained showed increases in DNA damage, both primary and oxidative, after treatment with oleic acid-coated ION, even though the highest concentrations were found to interfere with OGG1 enzyme activity in incomplete cell culture medium. The results of this study encourage the need for checking the suitability of comet assay when used for testing genotoxicity of nanomaterials. Further investigations are required to assess the ability of ION to induce oxidative stress, and to elucidate the specific mechanism involved in primary DNA damage induced by these ION.

2015Conference object

Open access

Micronucleus and H2AX phosphorylation assessment of silica-coated iron oxide nanoparticles in human neuronal cells

Publication . Sánchez-Flores, Maria; Valdiglesias, Vanessa; Kiliç, Gozde; Costa, Carla; Fernandez-Bertolez, Natalia; Costa, Solange; Teixeira, João Paulo; Pasaro, Eduardo; Laffon, Blanca

As clinically approved metal oxide nanoparticles, iron oxide nanoparticles (ION) hold immense potential in a vast variety of applications in various fields of biomedicine and biotechnology. With the increase in ION usage, particularly in diagnostics and therapeutics, concerns regarding their interactions with cellular components and possible deleterious effects are also growing.

2015-08Conference object

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Oxidative stress induced by silica-coated iron oxide nanoparticles in SHSY5Y neuronal cells

Publication . Kiliç, G.; Costa, C.; Fernández-Bertólez, N.; Costa, S.; Teixeira, Joao Paulo; Pásaro, E.; Laffon, B.; Vdz Park, M.; Valdiglesias, V.

Nanotechnology industry is progressing with prospects of substantial benefits to economics and science. Superparamagnetic iron oxide nanoparticles (ION) have been showing excellent magnetic properties, biocompatibility and biodegradability, broadening their potential applications and importance in the biomedical field. Nevertheless, there are increasing concerns as to the potential adverse effects on human health and environment and, currently, data on the effects of ION on the human nervous system are controversial.

2015-09Conference object

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Silica-coated iron oxide nanoparticles do not induce DNA double strand breaks or aneugenicity in SHSY5Y neuronal cells

Publication . Sánchez-Flores, M.; Kiliç, G.; Costa, C.; Fernández-Bertólez, N.; Costa, S.; Teixeira, João Paulo; Pásaro, E.; Valdiglesias, V.; Laffon, B.

Since increasing biomedical applications, both diagnostic and therapeutic, of ION are being developed, it is crucial to know how they interact with the cellular material and the possible consequences derived for human health.

2015-10Conference object

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Toxicity of gold nanorods on zebrafish (Danio rerio) embryos

Publication . Mesquita, B.; Fraga, S.; Simões, A.M.; Lopes, I.; Teixeira, J.P.

Gold nanoparticles (NPs) are being incorporated into various consumer products and are very promising in biomedicine as diagnostic and therapeutic delivery platforms. However, concerns about their safety, environmental and health impact have risen, as they might establish harmful interactions with biological systems. In this context, it is of major importance to understand the implications of exposure to these NPs on early life stages of biota, which have been considered, in general, as the most sensitive to chemical contamination. The present study aimed to evaluate the lethal and sublethal effects that gold nanorods (GNRs) may provoke on early-life stages of the fish species Danio rerio.

2016-06-21Conference object

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