Browsing by Issue Date, starting with "2019-06-04"
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- Hospitalization Risk Due to Respiratory Illness Associated with Genetic Variation at IFITM3 in Patients with Influenza A(H1N1)pdm09 Infection: a Case-Control StudyPublication . Gaio, Vânia; Nunes, Baltazar; Pechirra, Pedro; Conde, Patrícia; Guiomar, Raquel; Matias Dias, Carlos; Barreto, MartaRecent studies suggest an association between the Interferon Inducible Transmembrane 3 (IFITM3) rs12252 variant and the course of influenza infection. However, it is not clear whether the reported association relates to influenza infection severity. The aim of this study was to estimate the hospitalization risk associated with this variant in Influenza Like Illness (ILI) patients during the H1N1 pandemic influenza. A case-control genetic association study was performed, using nasopharyngeal/oropharyngeal swabs collected during the H1N1 pandemic influenza. Laboratory diagnosis of influenza infection was performed by RT-PCR, the IFITM3 rs12252 was genotyped by RFLP and tested for association with hospitalization. Conditional logistic regression was performed to calculate the confounder-adjusted odds ratio of hospitalization associated with IFITM3 rs12252. We selected 312 ILI cases and 624 matched non-hospitalized controls. Within ILI Influenza A(H1N1)pdm09 positive patients, no statistical significant association was found between the variant and the hospitalization risk (Adjusted OR: 0.73 (95%CI: 0.33–1.50)). Regarding ILI Influenza A(H1N1)pdm09 negative patients, CT/CC genotype carriers had a higher risk of being hospitalized than patients with TT genotype (Adjusted OR: 2.54 (95%CI: 1.54–4.19)). The IFITM3 rs12252 variant was associated with respiratory infection hospitalization but not specifically in patients infected with Influenza A(H1N1)pdm09.
- Safety assessment of nanofibrillated cellulose to human health using cellular modelsPublication . Ventura, Célia; Lourenço, Ana Filipa; Vilar, Madalena; Teixeira, Sara; Ferreira, Paulo J.T.; Silva, Maria JoãoNanomaterial - A natural, incidental or manufactured material containing particles, in an unbound state or as an aggregate or as an agglomerate and where, for 50% or more of the particles in the number size distribution, one or more external dimensions is in the size range 1 nm - 100 nm. In specific cases and where warranted by concerns for the environment, health, safety or competitiveness the number size distribution threshold of 50% may be replaced by a threshold between 1 and 50%. (2011/696/EU)
- Hospitalization Risk Due to Respiratory Illness Associated with Genetic Variation at IFITM3 in Patients with Influenza A(H1N1)pdm09 Infection: a CaseControl StudyPublication . Gaio, VâniaBackground: Recent studies suggest an association between the Interferon Inducible Transmembrane 3 (IFITM3) rs12252 variant and the course of influenza infection. However, it is not clear whether the reported association relates to influenza infection severity. The aim of this study was to estimate the hospitalization risk associated with this variant in Influenza Like Illness (ILI) patients during the H1N1 pandemic influenza.
- Gripe: interação do vírus influenza com o seu hospedeiro humanoPublication . David, SusanaA gripe é responsável por 3 a 5 milhões de casos de doença grave, com 290 000 a 650 000 mortes anuais a nível mundial. A Organização Mundial da Saúde decretou uma prioridade nos estudos sobre o papel da genética do hospedeiro na suscetibilidade a formas clinicamente graves da infeção pelo vírus da influenza.
- Network Biology Identifies Novel Regulators of CFTR Trafficking and Membrane StabilityPublication . Loureiro, Cláudia Almeida; Santos, João D.; Matos, Ana Margarida; Jordan, Peter; Matos, Paulo; Farinha, Carlos M.; Pinto, Francisco R.In cystic fibrosis, the most common disease-causing mutation is F508del, which causes not only intracellular retention and degradation of CFTR, but also defective channel gating and decreased membrane stability of the small amount that reaches the plasma membrane (PM). Thus, pharmacological correction of mutant CFTR requires targeting of multiple cellular defects in order to achieve clinical benefit. Although small-molecule compounds have been identified and commercialized that can correct its folding or gating, an efficient retention of F508del CFTR at the PM has not yet been explored pharmacologically despite being recognized as a crucial factor for improving functional rescue of chloride transport. In ongoing efforts to determine the CFTR interactome at the PM, we used three complementary approaches: targeting proteins binding to tyrosine-phosphorylated CFTR, protein complexes involved in cAMP-mediated CFTR stabilization at the PM, and proteins selectively interacting at the PM with rescued F508del-CFTR but not wt-CFTR. Using co-immunoprecipitation or peptide-pull down strategies, we identified around 400 candidate proteins through sequencing of complex protein mixtures using the nano-LC Triple TOF MS technique. Key candidate proteins were validated for their robust interaction with CFTR-containing protein complexes and for their ability to modulate the amount of CFTR expressed at the cell surface of bronchial epithelial cells. Here, we describe how we explored the abovementioned experimental datasets to build a protein interaction network with the aim of identifying novel pharmacological targets to rescue CFTR function in cystic fibrosis (CF) patients. We identified and validated novel candidate proteins that were essential components of the network but not detected in previous proteomic analyses.
- INSaFLU: a bioinformatics framework that potentiates the integration of influenza genome sequencing data for an enhanced and harmonized global flu surveillancePublication . Borges, VitorProcurarei dar um perspetiva geral da importância da análise total do genoma viral na vigilância laboratorial (e actividades de I&D) do vírus influenza, destacando o potencial papel da plataforma INSaFLU nesta revolução tecnológica / científica. Abordarei ainda alguns desafios (e perspetivas futuras) associados à integração da informação gerada com a sequenciação total do genoma de agentes patogénicos na descodificação da interação destes agentes com o seu hospedeiro humano.