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- Prevalência de demências na população sob observação da Rede de Médicos-Sentinela em 2006Publication . Rodrigues, Ana Paula; Sousa-Uva, Mafalda; Galvão, Cristina; Nunes, Baltazar; Dias, Carlos Matias
- Vigilância laboratorial das infeções por Neisseria gonorrhoeae em Portugal, 2004-2013Publication . Rodrigues, J.C.; Reis, L.; Cordeiro, D.; João, Inês; Diniz, M.; Nunes, A.; Florindo, C.; Borges, V.; Ferreira, R.; Pinto, M.; Varela, M.; Gomes, João Paulo; Borrego, M.J.; Rede para a Coleção Nacional de Neisseria gonorrhoeae
- Is organic farming safer to farmers' health? A comparison between organic and traditional farmingPublication . Costa, C.; García-Lestón, J.; Costa, S.; Coelho, P.; Silva, S.; Pingarilho, M.; Valdiglesias, V.; Mattei, F.; Dall'Armi, V.; Bonassi, S.; Laffon, B.; Snawder, J.; Teixeira, João PauloExposure to pesticides is a major public health concern, because of the widespread distribution of these compounds and their possible long term effects. Recently, organic farming has been introduced as a consumer and environmental friendly agricultural system, although little is known about the effects on workers' health. The aim of this work was to evaluate genetic damage and immunological alterations in workers of both traditional and organic farming. Eighty-five farmers exposed to several pesticides, thirty-six organic farmers and sixty-one controls took part in the study. Biomarkers of exposure (pyrethroids, organophosphates, carbamates, and thioethers in urine and butyrylcholinesterase activity in plasma), early effect (micronuclei in lymphocytes and reticulocytes, T-cell receptor mutation assay, chromosomal aberrations, comet assay and lymphocytes subpopulations) and susceptibility (genetic polymorphisms related to metabolism - EPHX1, GSTM1, GSTT1 and GSTP1 - and DNA repair-XRCC1 and XRCC2) were evaluated. When compared to controls and organic farmers, pesticide farmers presented a significant increase of micronuclei in lymphocytes (frequency ratio, FR=2.80) and reticulocytes (FR=1.89), chromosomal aberrations (FR=2.19), DNA damage assessed by comet assay (mean ratio, MR=1.71), and a significant decrease in the proportion of B lymphocytes (MR=0.88). Results were not consistent for organic farmers when compared to controls, with a 48% increase of micronuclei in lumphocytes frequency (p=0.016) contrasted by the significant decreases of TCR-Mf (p=0.001) and %T (p=0.001). Our data confirm the increased presence of DNA damage in farmers exposed to pesticides, and show as exposure conditions may influence observed effects. These results must be interpreted with caution due to the small size of the sample and the unbalanced distribution of individuals in the three study groups.
- Isolation and molecular characterization of Toxoplasma gondii isolated from pigeons and stray cats in Lisbon, PortugalPublication . Vilares, A.; Gargaté, M.J.; Ferreira, I.; Martins, S.; Júlio, C.; Waap, H.; Angelo, H.; Gomes, João PauloCats and pigeons are important factors in the epidemiology of Toxoplasma gondii as felidsare the only definitive hosts that can excrete environmentally resistant oocysts, andpigeons share the same places of cats and humans constituting a good model and indicatorof the ground field contamination. We aimed to study the virulence and genotypes ofT. gondii isolated from pigeons and stray cats in Lisbon, Portugal. Fresh samples of brainfrom 41 pigeons and 164 cats revealing antibodies to T. gondii were inoculated in mice.Three isolates (one isolated from a cat and two isolated from pigeons) were virulent in themouse model. Sag2-based genotyping of T. gondii was achieved in 70.7% (29/41) of samplesisolated from pigeons (26 samples were type II, two were type III, and one strain was type I).From the cat brain samples, 50% (82/164) yielded Sag2 positive results, where 72 belongedto genotype II and 10 were no type III (it was not possible to discriminate between typeI and II). Further genotyping was obtained by multiplex PCR of 5 microsatellites (TUB2,TgM-A, W35, B17, B18), allowing the identification of two recombinant strains that hadbeen previously identified as type II by Sag2 amplification (one isolated from cat brain andthe other from pigeon brain). This is the first evidence of recombinant strains circulatingin Portugal and the first report of T. gondii genotyping from cats in this country. This studyalso highlights the importance of environmental contamination in the synanthropic cycleconstituting a potential source of human infection.
- Boletim Epidemiológico Observações: Vol. 3, Nº10, out-dez 2014Publication . Instituto Nacional de Saúde Doutor Ricardo Jorge, IPObservações é uma publicação científica trimestral do INSA, IP, que visa contribuir para o conhecimento da saúde da população, os fatores que a influenciam, a decisão e a intervenção em Saúde Pública, assim como a avaliação do seu impacte na população portuguesa. Através do acesso público e gratuito a resultados científicos gerados por atividades de observação em saúde, monitorização e vigilância epidemiológica, é dada especial atenção à disseminação rápida de informação relevante para a resposta a temas de relevo para a saúde da população portuguesa, tendo como principal alvo todos os profissionais, investigadores e decisores intervenientes na área da Saúde Pública em Portugal.
- Isolation and molecular characterization of Toxoplasma gondii isolated from pigeons and stray cats in Lisbon, PortugalPublication . Vilares, Anabela; Gargaté, Maria João; Ferreira, Idalina; Martins, Susana; Júlio, Cláudia; Waap, Helga; Ângelo, Helena; Gomes, João PauloCats and pigeons are important factors in the epidemiology of Toxoplasma gondii as felids are the only definitive hosts that can excrete environmentally resistant oocysts, and pigeons share the same places of cats and humans constituting a good model and indicator of the ground field contamination. We aimed to study the virulence and genotypes of T. gondii isolated from pigeons and stray cats in Lisbon, Portugal. Fresh samples of brain from 41 pigeons and 164 cats revealing antibodies to T. gondii were inoculated in mice. Three isolates (one isolated from a cat and two isolated from pigeons) were virulent in the mouse model. Sag2-based genotyping of T. gondii was achieved in 70.7% (29/41) of samples isolated from pigeons (26 samples were type II, two were type III, and one strain was type I). From the cat brain samples, 50% (82/164) yielded Sag2 positive results, where 72 belonged to genotype II and 10 were no type III (it was not possible to discriminate between type I and II). Further genotyping was obtained by multiplex PCR of 5 microsatellites (TUB2, TgM-A, W35, B17, B18), allowing the identification of two recombinant strains that had been previously identified as type II by Sag2 amplification (one isolated from cat brain and the other from pigeon brain). This is the first evidence of recombinant strains circulating in Portugal and the first report of T. gondii genotyping from cats in this country. This study also highlights the importance of environmental contamination in the synanthropic cycle constituting a potential source of human infection.
- Alteração de marcadores inflamatórios, imunidade inata e metabolismo do ferro numa população portuguesa com doença de BehçetPublication . Oliveira, Rita; Napoleão, Patricia; Banha, João; Pereira, Dina; Barcelos, Filipe; Teixeira, Ana; Patto, José Vaz; Crespo, Ana Maria Viegas; Costa, Luciana
- Doença meningocócica do serogrupo B (MenB) em Portugal: uma reflexão sobre estratégias de imunizaçãoPublication . Simões, Maria João; Fernandes, Teresa; Gonçalves, Paulo; Bettencourt, Célia; Furtado, Cristina
- Variantes genéticas da hormona hepcidina contribuem para aumentar os níveis da ferritina sérica em indivíduos suscetíveis de desenvolverem sobrecarga em ferroPublication . Silva, Bruno; Pita, Lina; Gomes, Susana; Loureiro, Pedro; Gonçalves, João; Faustino, Paula