Browsing by Author "Vilela, Regina"
Now showing 1 - 4 of 4
Results Per Page
Sort Options
- Development of an antisense oligonucleotide-mediated exon skipping therapeutic strategy for Mucolipidosis II: validation at RNA levelPublication . Matos, Liliana; Vilela, Regina; Rocha, Melissa; Santos, Juliana Inês; Coutinho, Maria Francisca; Gaspar, Paulo; Prata, Maria João; Alves, SandraLysosomal Storage Disorders (LSDs) are a group of rare inherited metabolic diseases caused by the malfunction of the lysosomal system, which results in the accumulation of undegraded substrates inside the lysosomes and leads to severe and progressive pathology. Despite there is currently a broad understanding of the molecular defects behind LSDs, curative therapies have been approved for only few of these diseases whereas existing treatments are still mostly symptomatic with several limitations. Mucolipidosis type II alpha/beta (ML II) is one of most severe LSDs, which is caused by the total deficiency of the GlcNAc-1-phosphotransferase, a key enzyme for the formation of specific targeting signals on lysosomal hydrolases to lysosomes. GlcNAc-1-phosphotransferase is a multimeric enzyme complex encoded by two genes: GNPTAB and GNPTG. One of the most frequent ML II causal mutation is a dinucleotide deletion on exon 19 of GNPTAB (c.3503_3504del) that leads to the generation of a truncated protein, loss of GlcNAc-1-phosphotransferase activity, and missorting of multiple lysosomal enzymes. Presently, there is no therapy available for ML II. In this study we explored the possibility of an innovative therapeutic strategy for ML II based on the use of antisense oligonucleotides (AOs) capable to induce the skipping of GNPTAB exon 19 harboring the most common disease-causing mutation, c.3503_3504del. The approach confirmed the ability of specific AOs for RNA splicing modulation, thus paving the way for future studies on the therapeutic potential of this strategy.
- Development of an antisense-mediated exon skipping approach as a therapeutic option for the ML II-causing mutation c.3503_3504delTCPublication . Matos, Liliana; Vilela, Regina; Coutinho, Maria Francisca; Gaspar, Paulo; Alves, SandraLysosomal storage disorders (LSDs) are a group of a rare inherited metabolic disorders.
- Development of an antisense-mediated exon skipping therapeutic strategy to correct a frequent causing mutation in Mucolipidosis IIPublication . Matos, Liliana; Vilela, Regina; Coutinho, Maria Francisca; Gaspar, Paulo; Alves, SandraLysosomal Storage Disorders (LSDs) are a group of rare inherited diseases caused by the malfunction of the lysosomal system, resulting in the accumulation of undegraded substrates inside the lysosomes and leading to severe and progressive pathology. Among them is Mucolipidosis II (ML II), one of most severe LSDs, which is caused by the total or near total deficiency of the GlcNAc-phosphotransferase, a key enzyme for the correct trafficking of lysosomal hydrolases to the lysosome. GlcNAc-phosphotransferase is a multimeric enzyme and is encoded by two genes: GNPTAB and GNPTG. One of the most frequent ML II causal mutations is a dinucleotide deletion on exon 19 of the GNPTAB gene that disrupts the reading frame and prevents the production of an active GlcNAc-phosphotransferase, which in turn impairs the proper targeting of lysosomal enzymes. Despite broad understanding of the molecular causes behind this and other LSDs, the same progress has not been observed in the development of new therapies, with current treatments still mostly symptomatic and presenting several limitations. Therefore, alternative options should be investigated in order to provide patients and families with better healthcare and more promising therapies. One possibility is the modulation of splicing by antisense oligonucleotides (AOs) with the purpose of altering the splicing pattern, the mature mRNA and ultimately the final protein product. Acknowledging this, the present study intends to design and develop a RNA-based therapeutic agent through the use of AOs capable of inducing the skipping of exon 19 of the GNPTAB gene and consequently circumvent the effects of the most common ML II causal mutation. The approach is presently ongoing and different 2’O-Methyl AOs were designed and synthesized to target the GNPTAB exon 19 and promote its skipping. We have already succeeded in inducing the skipping of exon 19 in control and ML II patient fibroblasts. At biochemical level, 48 hours following transfection, enzyme activity suffered a small increase in patients fibroblasts for all enzymes tested, even if the results are still much lower than the observed for healthy controls. In conclusion, this work constitutes a proof of principle for correcting in vitro the defects caused by a frequent ML II mutation with antisense therapy. Acknowledgments: This work is supported by the project PTDC /BBB-BMD/6301/2014 (SPLICETHER) financed by Fundação para a Ciência e a Tecnologia – Portugal.
- Use of an antisense-mediated exon skipping approach as a therapeutic option for a common Mucolipidosis type II causing mutationPublication . Matos, Liliana; Vilela, Regina; Coutinho, Maria Francisca; Gaspar, Paulo; Alves, SandraLysosomal Storage Disorders (LSDs) are a group of rare inherited diseases caused by the malfunction of the lysosomal system, resulting in the accumulation of undegraded substrates inside the lysosomes and leading to severe and progressive pathology. Among them is ML II, one of most severe LSDs, which is caused by the total or near total deficiency of the GlcNAc-phosphotransferase, a key in enzyme for the correct trafficking of lysosomal hydrolases to the lysosome. GlcNAc-phosphotransferase is a multimeric enzyme and is encoded by two genes: GNPTAB and GNPTG. One of the most frequent ML II causal mutations is a dinucleotide deletion on exon 19 of the GNPTAB gene that disrupts the reading frame and prevents the production of an active GlcNAc-phosphotransferase, which in turn impairs the proper targeting of lysosomal enzymes. Despite broad understanding of the molecular causes behind this and other LSDs, the same progress has not been observed in the development of new therapies, with current treatments still mostly symptomatic and presenting several limitations. Therefore, alternative options should be investigated in order to provide patients and families with better healthcare and more promising therapies. One possibility is the modulation of splicing by antisense oligonucleotides with the purpose of altering the splicing pattern, the mature mRNA and ultimately the final protein product. Acknowledging this, the present study intends to design and develop a RNA-based therapeutic agent through the use of antisense oligonucleotides capable of inducing the skipping of exon 19 of the GNPTAB gene and consequently circumvent the effects the most common ML II causal mutation. The approach is presently ongoing and different 2’O-Methyl AOs were designed and synthesized to target the GNPTAB exon 19 and promote its skipping. We have already succeed in inducing the skipping of exon 19 in control and ML II patient cell lines and we are now evaluating the effects of this therapeutic approach at biochemical levels.