Browsing by Author "Rebelo de Andrade, Helena"
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- A cost-efficient solution: reagent comparison guide for neuraminidase inhibition assayPublication . Louro, João; Correia, Vanessa; Rebelo de Andrade, HelenaObjectives: Compare available alternative reagents for Neuraminidase Activity Assay and Neuraminidase Inhibition Assay; Assess the phenotypic susceptibility profiles of influenza viruses from different (sub)types to the new Neuraminidase Inhibitors: laninamivir and peramivir.
- Functional compatibility of PB1 and antigenic proteins as a determinant of viral fitness and adaptation in the A(H1N1)pdm09Publication . Gíria, Marta; Louro, João; Rebelo de Andrade, HelenaObjectives: To determine if the functional compatibility between PB1 and antigenic proteins is a molecular determinant of virus growth and antigen yield in PR8:A(H1N1)pdm09 reassortants; To evaluate the phenotypic expression of reverting mutations R386K, I517V and L298I, previously identified as putatively associated with an enhanced functional compatibility between PB1 and HA in the A(H1N1)pdm09
- Reverse genetics vaccine seeds for influenza: Proof of concept in the source of PB1 as a determinant factor in virus growth and antigen yieldPublication . Gíria, Marta; Santos, Luís; Louro, João; Rebelo de Andrade, HelenaGrowth deficits of reverse genetics vaccine seeds have compromised effective immunization. The impairment has been attributed to sub-optimal protein interactions. Some level of dependence may exist between PB1 and antigenic glycoproteins, however, further research is necessary to clarify the extent to which it can be used in favor of seed production. Our objective was to establish proof of concept on the phenotypic outcome of PB1 source in the PR8: A(H1N1)pdm09 reassortants. Reassortants were generated with the gene constellation of the classical 6:2 PR8: HA, NApdm09 seed prototype and the 5:3 reassortant PR8: HA, NA, PB1pdm09. Viral growth and antigen yield were evaluated 12-60h post-infection. The 5:3 reassortant presented statistically significant growth and antigen yield improvements when compared to the 6:2. We believe these findings to be of promising value to vaccine research towards an improvement of reverse genetic seeds, an overall more cost-effective vaccine manufacture and timely delivery.
- Uncovering the role of positive selection in the evolutionary pathways to influenza virus resistance or reduced inhibition by neuraminidase inhibitorsPublication . Correia, Vanessa; Abecasis, Ana; Rebelo de Andrade, HelenaThis study aimed at estimating the site-specific SP on the NA gene of currently circulating human influenza viruses, in order to infer the SP acting specifically on the sites associated with (H)RI by NAIs and on further sites contacting directly or indirectly with the drug (active site). The frequency of drug-resistant and (H)RI variants was determined as a secondary objective, prompted by the need of determining the entire amino acid composition of the sites to understand SP data