Browsing by Author "Oliveira, S."
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- Development and validation of a multi-method for determination of phenolics in aromatic plantsPublication . Oliveira, S.; Castilho, M.C.; Ribeiro-Santos, R.; Ramos, F.; Sanches-Silva, A.Introduction: Aromatic plants attract great attention from public and scientific community due to their potential health benefits. Phenolic compounds greatly contribute for aromatic plants properties, including the prevention of cardiovascular and neurodegenerative diseases and cancer. The aim of this paper is the development and validation of a method of Ultra High Performance Liquid Chromatography coupled with Diode Array detector (UHPLC-DAD), which allows quantifying nineteen phenolic compounds simultaneously in aromatic plants.
- Variants in the inflammatory IL6 and MPO genes modulate stroke susceptibility through main effects and gene-gene interactionsPublication . Vicente, A.M.; Manso, H.; Krug, T.; Sobral, J.; Gaspar, G.; Oliveira, S.; Ferro, J.M.A complex interplay between genetic background, clinical and life-style factors and the environment is expected to ultimately regulate the onset, acute phase and outcome of stroke. There is substantial evidence that inflammation within the Central Nervous System contributes to stroke risk, and known clinical risk factors for stroke, like atherosclerosis, diabetes, obesity, hypertension, and peripheral infection, are associated with an elevated systemic inflammatory profile. The inflammatory response is equally of major importance in recovery and healing processes after stroke. In this study we tested the genetic association of major inflammatory players IL1B (2q14), IL6 (7p21), TNF (6p21.3) and MPO (17q23.1) with stroke susceptibility and with stroke outcome at three months, in a population sample of 672 patients and 530 controls, adjusting for demographic, clinical and life-style risk factors and/or stroke severity parameters. The apparent complexity of the inflammatory mechanisms in stroke, and the multiplicity of players involved suggest a concerted process, in which implicated molecules interact to tightly regulate each other. We therefore examined both independent gene effects and the occurrence of gene-gene interactions among the tested inflammatory genes in stroke risk and stroke recovery. Two IL6 and one MPO SNP were significantly associated with stroke risk after multiple testing correction (0.022 correctedP 0.042), highlighting gene variants of low to moderate effect in stroke risk. An epistatic interaction between the IL6 and MPO genes was also identified in association with stroke susceptibility (P=0.031 after 1000 permutations). In the subset of 546 patients assessed for stroke outcome at three months using the modified Rankin Scale (mRS), we found one IL6 haplotype associated with stroke outcome (correctedP=0.024). In the present study we present supporting evidence for a role of the IL6 and MPO inflammatory genes in stroke susceptibility, and show that stroke risk is modulated by main gene effects together with clinical and life-style factors as well as by gene-gene interactions. Our findings are compatible and strengthen previous genetic and biological observations, highlighting the need of further functional studies, particularly in view of the possible utility of IL-6 as a diagnostic and/or prognostic biomarker for stroke.