Browsing by Author "Martins, Marta"
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- Bromine occurrence in Portuguese cabbage from center region of Portugal before and after forest firesPublication . Ventura, Marta; Delgado, Inês; Coelho, Inês; Gueifão, Sandra; Matos, Ana Sofia; Martins, Marta; Costa, Maria Helena; Castanheira, IsabelBromine (Br) is one of the main halogens of the periodic table and has been classified as emerging contaminant due to its persistence in the environment, bio-magnification properties and risk to human health [1]. The main goal of this work was to evaluate Bromine contents in Portuguese cabbage (Brassica Oleracea var. Costata DC), in the center region of Portugal before and after forest fires. This vegetable is one of the most consumed by the Portuguese population and is recognized as an accumulator of contaminants from the air and from soils. All cabbage samples were collected in the center region of Portugal. Before forest fire 3 Portuguese cabbage were collected in 9 different zones in a total of 27 samples (Control Group-CG) and used as a control. After forest fire (AFF), 3 Portuguese cabbage were collected in 8 zones over four periods of time (first campaign in August of 2017, second campaign in December of 2017, third campaign in April of 2018 and fourth campaign in August of 2018), in a total of 96 samples. Bromine contents was determined by inductively coupled plasma mass spectrometry (ICP-MS), preceded by an alkaline extraction with 25% ammonium tetramethylhydroxide (TMAH). The results were obtained through analytical procedures that reflected the quality assurance requirements described in ISO / IEC 17025: 2005. The concentration was expressed in mg Br/kg cabbage. The amount of Br in CG (mean of 27 samples) was 2 mg/kg. Most of the cabbage samples collected after forest fire presented concentrations above CG, with the following values; the first campaign (4.8 mg/kg), the second campaign (4.0 mg/kg) and the fourth campaign (4.5 mg/kg). The only campaign AFF that presented values below CG was the third one (1.7 mg/kg). Results before forest fires (CG) and after forest fire (4 campaigns) using One Way ANOVA pointed successfully on the existing statistical differences between bromine concentrations in CG and AFFs collection (p-value < 0.05). Through the Least significant differences (LSD) test the 1st and 4th campaigns (AFF) were the only ones that evidenced significant differences with the CG. After the forest fires, the 3rd campaign showed significantly lowest values when compared with the 1st and the 4th campaigns. However, these results do not pose risk to the Portuguese population, according to the commission regulation (EC) No 839/2008, wherein the value of bromide in brassicas is 30mg/kg [2].
- Effect of season and proximate composition on the Br, As, Cd and Pb contents in different kinds of key foods consumed in PortugalPublication . Ventura, Marta; Cardoso, Carlos; Bandarra, Narcisa Maria; Delgado, Inês; Coelho, Inês; Gueifão, Sandra; Martins, Marta; Costa, Maria Helena; Castanheira, IsabelOn the basis of a Total Diet Study, the Br, As, Cd and Pb contents in relevant food groups consumed in Portugal were determined. The studied groups were meat products, fatty fish, lean fish, shellfish, cephalopods, fresh and dried fruit, crucifers and other vegetables. It was observed that the concentrations of As, Br, Cd and Pb in fruit, vegetables and meat products were much lower than in seafood. Fresh fruit exhibited the lowest elemental contents. The concentrations of As, Br, Cd and Pb did not show any dependence on season. With respect to proximate composition, elemental content dependence on protein or ash was weak. PCA showed relevant associations: between Br and ash contents in fatty fish; between Cd, Pb and protein contents in crucifers; and between As, fat and protein contents in the other vegetables group. Therefore, the effect of the proximate composition on the studied elemental contents warrants investigation.
- Enriched feeds with iodine and selenium from natural and sustainable sources to modulate farmed gilthead seabream (Sparus aurata) and common carp (Cyprinus carpio) fillets elemental nutritional valuePublication . Barbosa, Vera; Maulvault, Ana Luísa; Anacleto, Patrícia; Santosa, Marta; Mai, Mónica; Oliveira, Helena; Delgadoe, Inês; Coelho, Inês; Barata, Marisa; Araújo‐Lunaa, Ravi; Ribeiro, Laura; Eljasik, Piotr; Sobczak, Małgorzata; Sadowski, Jacek; Tórz, Agnieszka; Panicz, Remigiusz; Dias, Jorge; Pousão-Ferreira, Pedro; Carvalho, Maria Luísa; Martins, Marta; Marques, AntónioDeveloping tailor-made fortified farmed fish is a promising solution to overcome nutritional deficiencies and increase consumer confidence in these products. This study evaluated the supplementation of three fortified diets with I-rich seaweed and selenised-yeast on essential and toxic elements levels in gilthead seabream (Sparus aurata) and common carp (Cyprinus carpio). Fortified diets resulted in increased I, Se and Fe in fish muscle. Biofortified seabream and carp revealed lower Cu and Br. The reduction of fishmeal and fish oil in fortified diets resulted in lower Hg and Cd in seabream muscle. Contrarily, fortified diets increased As and Hg in carp fillets. The consumption of 150 g of fortified seabream enabled a significantly higher contribution to the daily recommended intake (DRI) of I (10%) and Se (76%) than non-fortified fish, whereas fortified carp fulfilled 23% of I DRI and 91% of Se DRI. Moreover, the exposure to Pb decreased with the consumption of biofortified seabream (23–82% BMDL01) and carp (26–92% BMDL01). These results support the strategy of developing eco-innovative biofortified farmed fish using sustainable, natural, safe and high-quality ingredients in feeds, to enable consumers to overcome nutritional deficiencies without significantly increased feed costs.
- HERA - Environmental Risk Assessment of a contaminated estuarine environment: a case studyPublication . Martinho, Ana Paula; Rodrigo, Ana; Vicente, Ana; Machado, Ausenda; Dias, Carlos Matias; Guiomar, Carla Sofia; Gonçalves, Cátia; Paixão, Eleonora; Santos, Fernanda; Louro, Henriqueta; Pinhal, Hermínia; Mateus, Inês; Coelho, Inês; Lopes, Inês; Castanheira, Isabel; Antunes, Joana; Sacadura, Joana; Lavinha, João; Toro, Joaquim; Lobo, Jorge; Nunes, Luís; Quintas, Maria do Carmo; Costa, Maria Helena; Silva, Maria João; Saraiva, Marina; Diniz, Mário; Martins, Marta; Pinto, Miguel; Fernandes, Paula Vaz; Costa, Pedro M.; Caeiro, Sandra; Gueifão, Sandra; carreira, Sara; Silva, Susana Pereira; Neuparth, TeresaSado River estuary is located in the west coast of Portugal. Previous environmental studies identified industrial contamination, non-point anthropogenic sources and contamination coming from the river, all promoting accumulation of polluted sediments with known impacts on the ecological system. Surrounding human populations have intense economic fishery activities. Together with agriculture, estuary fishing products are available to local residents. Food usage previously characterized through ethnographic studies suggests exposure to estuarine products, farming products, and water in daily activities, as potential routes of contamination. It is well established that long term exposure to heavy metals are associated with renal and neurological diseases, most heavy metals are classified as carcinogenic and teratogenic.
- In vitro bioaccessibility of macro and trace elements in biofortified and conventional farmed gilthead seabream (Sparus aurata) and common carp (Cyprinus carpio)Publication . Barbosa, Vera; Anacleto, Patrícia; Maulvault, Ana Luísa; Oliveira, Helena; Marmelo, Isa; Ferraris, Francesca; Raggi, Andrea; Ventura, Marta; Sobczak, Małgorzata; Eljasik, Piotr; Panicz, Remigiusz; Tsampa, Kalliopi; Karydas, Andreas G.; Carvalho, Maria Luisa; Martins, Marta; Cubadda, Francesco; Marques, AntónioBiofortification is a promising strategy to improve the nutrient profile of farmed fish but requires consideration of the nutrient bioaccessible fraction. In this study, the in vitro bioaccessibility of macro and trace elements was investigated in biofortified and conventional farmed gilthead seabream and common carp, also taking into account the effect of cooking (by steaming). Biofortification enhanced iodine and selenium levels in seabream and carp fillets. Steaming increased iodine and selenium contents in biofortified seabream, and increased selenium and decreased copper levels in biofortified carp. Higher iodine bioaccessibilty (> 80%) was observed in biofortified seabream compared to biofortified carp (45%). In both species, selenium, iron, and zinc bioaccessibility was ≥ 70%. Upon steaming iodine and iron bioaccessibility decreased in seabream, while selenium bioaccessibility decreased in carp. The consumption of steamed biofortified seabream and carp contributes to significantly higher daily intakes of iodine (up to 12% and 10%, respectively) and selenium (up to 54% and above 100%, respectively) compared to conventional counterparts. The present study demonstrates the potential of developing innovative biofortified farmed fish using natural sustainable feed ingredients to improve the intake of important nutrients for human health.
- Morte súbita neonatal num recém-nascido com perturbação do desenvolvimento intelectual ligada ao X tipo Nascimento por delecção do gene UBE2APublication . Furtado Gomes, Inês; Martins, Marta; Marques, Bárbara; Correia, Hildeberto; Sanchez, BrunoIntrodução: A perturbação do desenvolvimento intelectual ligada ao X (PDIX) tipo Nascimento é uma síndrome genética rara caracterizada por défice intelectual, dismorfismos craniofaciais característicos e anomalias congénitas, nomeadamente defeitos cardíacos e do tracto génito-urinário. Descrição do caso: Recém-nascido do sexo masculino, admitido com 12 horas de vida na Unidade de Cuidados Intensivos Neonatais por hipoglicémia e dificuldade alimentar. Gestação vigiada, com diagnóstico pré-natal de agenésia renal esquerda. Parto eutócico às 36 semanas sem necessidade de manobras de reanimação e com somatometria ao nascimento adequada à idade gestacional. No exame objectivo destacava-se hipotonia axial e dismorfismos craniofaciais e dos membros (Figura 1): fendas palpebrais amendoadas, inclinadas para cima, hipertelorismo; dorso nasal côncavo, narinas antevertidas; boca larga com cantos voltados para baixo e lábios finos; pavilhões auriculares hipoplásicos; inserção proximal dos polegares; pés com primeiros dedos largos e sobreposição do segundo e terceiro dedos. Durante as primeiras duas semanas de vida apresentou quadro de hipertensão pulmonar, colestase, dificuldade alimentar e má progressão ponderal. Identificada comunicação interventricular perimembranosa, sem sinais de disfunção cardíaca. Sem outras malformações identificadas. Paragem cardiorrespiratória súbita ao 20º dia de vida, sem resposta a medidas de suporte avançado de vida. Exame anatomopatológico post-mortem não conclusivo quanto à causa de morte. O estudo genético (Array-CGH) identificou uma delecção de 386 kb na região Xq24, englobando o gene UBE2A e 6 genes contíguos, compatível com PDIX tipo Nascimento. Conclusão: Actualmente, encontram-se descritos 58 casos de PDIX tipo Nascimento, dos quais apenas um com diagnóstico no período neonatal. Apesar de constituir uma entidade rara, apresenta um fenótipo característico, passível de ser identificado em idade precoce. O caso descrito corresponde ao primeiro relato de morte súbita neonatal num indivíduo com este síndrome, realçando a importância do reconhecimento clínico desta entidade. Um diagnóstico atempado permitirá uma abordagem clínica dirigida, o estabelecimento do prognóstico e o aconselhamento genético da família.
- Physicochemical properties of iodine and selenium biofortified Sparus aurata and Cyprinus carpio during frozen storagePublication . Barbosa, Vera; Camacho, Carolina; Oliveira, Helena; Anacleto, Patrícia; Maulvault, Ana Luisa; Delgado, Inês; Ventura, Marta; Dias, Jorge; Ribeiro, Laura; Pousão-Ferreira, Pedro; Eljasik, Piotr; Panicz, Remigiusz; Sobczak, Małgorzata; Tsampa, Kalliopi; Karydas, Andreas; Nunes, Maria Leonor; Carvalho, Maria Luísa; Martins, Marta; Marques, AntónioFish biofortification with natural ingredients like iodine-rich macroalgae and selenized-yeast is an excellent strategy to enhance the nutritional quality of farmed fish. This study aimed to assess the effect of frozen storage during 12-months on physicochemical quality of biofortified seabream (Sparus aurata) and carp (Cyprinus carpio). Frozen storage reduced iodine content in biofortified seabream fillets (17%), as well as selenium content in biofortified carp fillets (24%). Yet, biofortified fillets still presented enhanced iodine and selenium contents at the end of the storage period. Increased lipid oxidation (3.45 mg MDA kg-1 for seabream and 2.41 mg MDA kg-1 for carp) and decreased water holding capacity (23-29% for seabream and 14-23% for carp) was observed during storage, whereas major changes in colour and texture occurred after 45 days (seabream) and 225 days (carp) of storage. In general, biofortified fish fillets maintained their nutritional value and quality after 360 days of frozen storage.
- Study around the Barroso mine (Portugal): Baseline levels of lithium for assessing future exposure and risks from Li mining activityPublication . Jesus, Susana; Ventura, Marta; Assunção, Ricardo; Gueifão, Sandra; Delgado, Inês; Rego, Andreia; Ribeiro, Mariana; Martins, Marta; Neves, Orquídia; Coelho, InêsThe energetic green transition is increasing the demand for lithium (Li) exploitation. However, the Li supply faces challenges like limited reserves and environmental concerns. This pioneer study aims to characterize the Li concentrations in the region around the Barroso mine, in Portugal, by collecting and analyzing samples of cabbage, potato, drinking and irrigation water and soil from two nearby sites, and performing a preliminary exposure and risk assessment of local populations. Li levels ranged between 20 and 589 μg/kg in cabbages (n = 23), 2.3–21 μg/kg in potatoes (n =21), 1.1–5.9 μg/L in drinking water (n =10), 1.1–15 μg/L in irrigation water (n =23) and 35–121 mg/kg in soils (n =23). Significant differences in Li content between sampling sites were observed only for cabbage samples. The risk assessment revealed that none of the participants exceeded the provisional reference dose (p-RfD) (2 μg/kg bw/day), with a hazard quotient (HQ) <1, suggesting no health concerns for the population. It is expected that the studied area will be affected by the future expansion of the mine concession, thus this pioneer study is crucial for future research as it establishes a initial database for evaluating the potential impact of mining activity on the environment and the population’s exposure to Li.