Repository logo
 
Publication

Fluorescein 5-maleimide: label of food contact nanocomposites

2016-06Conference object Restricted access
Simple item page
dc.contributor.authorSilva, Ana Sanches
dc.contributor.authorVilarinho, Fernanda
dc.contributor.authorBuonocore, Giovanna
dc.date.accessioned2017-02-21T14:17:42Z
dc.date.available2017-02-21T14:17:42Z
dc.date.issued2016-06
dc.description.abstractEngineered nanomaterials (ENMs) are increasingly being used due to their physical and chemical properties that allow improving a wide range of consumer products. Although they present a vast number of benefits, they also present risks to human health and the environment. Therefore, there is the need of evaluating the risks of nanotechnologies in foods. To fulfill this require new techniques are being developed. Fluorescent labeling is a simple approach for particle tracking that presents low detection limits. A fluorescent organic dye can be directly incorporated into layered silicates like montmorillonite (MMT) by ionic exchange but this can be dislodged from the substrate during the extrusion process. However the covalent bond of the fluorescent tag to the nanoclay can be a good alternative because it provides stability between the fluorescent tag and the clay. The main objective of this study was to develop an analytical method by Ultra High Performance Liquid Chromatographic (UHPLC) to determine fluorescein 5-maleimide, a fluorescent compound, as potential label of MMT. Conditions like chromatographic column, column temperature, mobile phase composition, mobile phase gradient as well as detector conditions were tested. Fluorescence detector and diode array detector (DAD) were compared. In general good linearity was found, excellent determination coefficients were achieved in all cases. Regarding the response of both detectors, as expected, better response was also obtained with fluorescence detector, allowing obtaining lower limits of quantification. The quantification limits of fluorescein 5-maleimide with the fluorescence detector were, at least, 10 times lower than with the DAD detector. Different behaviour was found in the two solvents tested. Ethanolic solutions allowed obtaining lower detection limits (LOD) than methanolic solutions (LOD ethanol = 0.03 µg/mL; LOD methanol =0.06 µg/mL, using fluorescence detector).pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/4332
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectSegurança Alimentarpt_PT
dc.subjectFluorescent Labelingpt_PT
dc.subjectFluorescein 5-maleimidept_PT
dc.subjectMontmorillonitept_PT
dc.subjectNanoclaypt_PT
dc.titleFluorescein 5-maleimide: label of food contact nanocompositespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceLisboa, Portugalpt_PT
oaire.citation.titleInternational Conference on Safety and Innovation in Food Packaging" (InSIPack), INSA, 16th June 2016pt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typeconferenceObjectpt_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
POSTER_FLUORESCEIN 5-MALEIMIDE LABEL OF FOOD CONTACT NANOCOMPOSITES.pdf
Size:
667.23 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DAN - Posters/abstracts em congressos internacionais